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Effect of exhaustive ultra-endurance exercise in muscular glycogen and both Alpha1 and Alpha2 Ampk protein expression in trained rats

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Abstract

Glycogen is the main store of readily energy in skeletal muscle and plays a key role in muscle function, demonstrated by the inability to sustain prolonged high-intensity exercise upon depletion of these glycogen stores. With prolonged exercise, glycogen depletion occurs and 5′-AMP-activated protein kinase (AMPK), a potent regulator of muscle metabolism and gene expression, is activated promoting molecular signalling that increases glucose uptake by muscular skeletal cells. The aim of this study was primarily to determine the effect of ultra-endurance exercise on muscle glycogen reserves and secondly to verify the influence of this type of exercise on AMPK protein expression. Twenty-four male Wistar rats, 60 days old, were divided into four experimental groups: sedentary, sedentary exhausted (SE), endurance trained (T) and endurance trained exhausted (TE). The animals ran for 10 to 90 min/day, 5 days/week, for 12 weeks to attain trained status. Rats were killed immediately after the exhaustion protocol, which consisted of running on a treadmill (at approximately 60 % V max until exhaustion). Optical density of periodic acid-Schiff was detected and glycogen depletion observed predominantly in type I muscle fibres of the TE group and in both type I and II muscle fibres in the SE group. Plasma glucose decreased only in the TE group. Hepatic glycogen was increased in T group and significantly depleted in TE group. AMPK protein expression was significantly elevated in TE and T groups. In conclusion, acute exhaustive ultra-endurance exercise promoted muscle glycogen depletion. It seems that total AMPK protein and gene expression is more influenced by status training.

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Acknowledgments

The authors would like to thank Fundação de Amparo a Pesquisa do Estado de São Paulo and Conselho Nacional de Desenvolvimento científico e Tecnológico.

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Authors and Affiliations

  1. Departamento de ortopedia e traumatologia da Universidade Federal de São Paulo, UNIFESP-EPM, São Paulo, Brazil

    V. A. F. Tarini, B. Schmidt & F. Faloppa

  2. Departamento de fisiologia do exercício e neurofisiologia da Universidade Federal de São Paulo, UNIFESP-EPM, São Paulo, Brazil

    R. M. Arida, C. A. Cunha & E. S. Alves

  3. Instituto de Ciências Biológicas da Universidade de São Paulo, ICB-USP, São Paulo, Brazil

    L. C. Carnevali Jr. & M. C. L. Seeleander

  4. Rua Borges Lagoa, 783, 5º andar—Vila Clementino, CEP: 04038-032, São Paulo, São Paulo, Brazil

    V. A. F. Tarini

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  1. V. A. F. Tarini
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Tarini, V.A.F., Carnevali, L.C., Arida, R.M. et al. Effect of exhaustive ultra-endurance exercise in muscular glycogen and both Alpha1 and Alpha2 Ampk protein expression in trained rats. J Physiol Biochem 69, 429–440 (2013). https://doi.org/10.1007/s13105-012-0224-5

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