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Correlating Cerebral 18FDG PET-CT Patterns with Histological Analysis During Early Brain Injury in a Rat Subarachnoid Hemorrhage Model

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Abstract

Early brain injury (EBI) plays a significant role in poor outcomes for subarachnoid hemorrhage (SAH) patients. Further investigations are required to characterize the cellular metabolic and related histological changes that may contribute to EBI following SAH. We investigated the image patterns of 18-fluorodeoxyglucose positron emission tomography-computed tomography (18FDG PET-CT) during EBI and correlated histopathological changes utilizing a rat SAH model. SAH was induced in six adult male Sprague-Dawley rats by endovascular perforation, and animals were randomly assigned to receive 18FDG PET-CT imaging at either 3 or 12 h post-procedure. Mean 18FDG standard uptake value (SUV) of the brain was calculated. Animals were euthanized 48 h post-procedure, and brain samples were used for heme oxygenase-1 (HO-1) and dopamine- and cAMP-regulated phosphoprotein (DARPP-32) Mr 32 kDa immunohistochemistry. Rats within the SAH group had higher mean whole brain 18FDG SUV (2.349 ± 0.376 g/ml in the 3-h group and 2.453 ± 0.495 g/ml in the 12-h group) compared to that of sham (n = 3; mean SUV = 2.030 ± 0.247 g/ml; P < 0.05) or control groups (n = 3; mean SUV = 1.800 ± 0.484 g/ml; P < 0.05). Whole brain 18FDG SUV did not vary significantly between rats imaged at 3 h vs. those imaged at 12 h post-SAH (P > 0.05). Regions of decreasing SUV in SAH rats correlated with neuronal death and increased expression of HO-1. Higher 18FDG PET SUV was evident in rats post-SAH compared to sham and control groups. Regions of decreasing SUV in SAH rats correlated with neuronal death and increased HO-1 expression as evaluated by histopathology.

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Conflict of Interest

The authors have no conflicts of interest to report pertaining to the materials or methods used in this study or the findings specified in this paper.

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Authors and Affiliations

  1. Department of Neurosurgery, University of Michigan, 1500 E. Medical Center Dr., Room 3552 TC, Ann Arbor, MI, 48109-5338, USA

    Jianping Song, Peiliang Li, B. Gregory Thompson, Guohua Xi & Aditya S. Pandey

  2. Department of Radiology, University of Michigan, Ann Arbor, MI, USA

    Neeraj Chaudhary & Joseph J. Gemmete

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  1. Jianping Song
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  2. Peiliang Li
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  3. Neeraj Chaudhary
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  4. Joseph J. Gemmete
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  5. B. Gregory Thompson
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  6. Guohua Xi
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  7. Aditya S. Pandey
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Correspondence to Aditya S. Pandey.

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Song, J., Li, P., Chaudhary, N. et al. Correlating Cerebral 18FDG PET-CT Patterns with Histological Analysis During Early Brain Injury in a Rat Subarachnoid Hemorrhage Model. Transl. Stroke Res. 6, 290–295 (2015). https://doi.org/10.1007/s12975-015-0396-8

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  • DOI: https://doi.org/10.1007/s12975-015-0396-8

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