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Regeneration in sugarcane via somatic embryogenesis and genomic instability in regenerated plants

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Abstract

In the present study, embryogenic calli of sugarcane variety BL4 were induced using 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin in different concentrations and combinations. In contrast to earlier studies, embryogenic callus sectors were identified and isolated microscopically within 1–2 weeks. Subsequently, 51 media formulations were used for regeneration of proliferated embryogenic callus, using MS medium supplemented with three different cytokinins [kinetin, 6-Benzylamino purine (BAP), and thidiazuron (TDZ)] and auxins (IAA/NAA and IBA) in different combination and concentrations. After acclimatization, the genomic DNA of regenerated plants was studied to explore the insertion polymorphism of transposable elements in order to ascertain the variation among somaclones. Though low concentration of kinetin with 2,4-D was found supportive to embryogenic callus development, the highest number of regenerated plantlets was observed using BAP (1 μM), however the plantlets had very low fresh weight (2.2 g). Conversely, TDZ alone supported a significant increase in the number of plantlets regenerated (38–40) with higher fresh weight. The somaclones generated during this study showed considerable positional polymorphism of activator-like transposable elements possibly due to the stress associated with in vitro culture. This study provides a procedure to produce regenerated sugarcane plants from embryogenic callus in a relatively short time.

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Authors and Affiliations

  1. The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan

    Saboohi Raza, Syeda Qamarunisa, Ishrat Jamil, Sadia Anjum, Abid Azhar & Javed Ahmed Qureshi

  2. Nuclear Institute of Agriculture (NIA), Tandojam, Pakistan

    Saboohi Raza

  3. Department of Molecular Pathology, Dow University of Health Sciences, Karachi, Pakistan

    Mushtaq Hussain

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  1. Saboohi Raza
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  2. Syeda Qamarunisa
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  3. Mushtaq Hussain
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  4. Ishrat Jamil
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  5. Sadia Anjum
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  6. Abid Azhar
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  7. Javed Ahmed Qureshi
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Correspondence to Saboohi Raza.

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Raza, S., Qamarunisa, S., Hussain, M. et al. Regeneration in sugarcane via somatic embryogenesis and genomic instability in regenerated plants. J. Crop Sci. Biotechnol. 15, 131–136 (2012). https://doi.org/10.1007/s12892-011-0111-6

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  • DOI: https://doi.org/10.1007/s12892-011-0111-6

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