Abstract
Thirty polymorphic microsatellites were developed and characterised for the aquaculture important species, Green chromide, Etroplus suratensis through Illumina Sequencing with a mean read quality (Phred score) of 35.8 and the microsatellite primers were designed from the sequenced information, considering various attributes such as the number of repeats, Tm, GC content, Product size, etc. using the software PRIMER 3.0 web tool. The allele numbers ranged from 6 to 18. The mean observed heterozygosity was 0.745 and the mean expected heterozygosity was 0.762. The average PIC value for 30 microsatellite primers was 0.80 and all the loci had PIC values > 0.75. The identified polymorphic loci would help in revealing the genetic stock identification among the Pearlspot population.
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The work was funded by the Agency for Aquaculture Development in Kerala, Government of Kerala, India.
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Magdeline christo: Has done sampling, data analysis, and draft preparation. Divya Merin Jose: has done laboratory work. P. R. Divya: has done Sampling. M. U. Rekha: conceived the concept and guidance to work. U. K Sarkar: Guidance and facilitating the work.
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Christo, M., Jose, D.M., Divya, P.R. et al. Development of polymorphic microsatellite markers for genetic stock identification of green chromide, Etroplus suratensis using next generation sequencing technology. Conservation Genet Resour (2024). https://doi.org/10.1007/s12686-024-01350-w
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DOI: https://doi.org/10.1007/s12686-024-01350-w