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Development and characterization of 123 SNPs in an endangered fish Semilabeo obscurus

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Abstract

Semilabeo obscurus, as an endangered species, is a certain economic fish in China and Vietnam. However, study on its genetic diversity is still lacking. In this study, 168 candidate SNPs were verified by Sanger-sequencing. Of these, 123 polymorphic SNP markers were successfully genotyped. The observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information conten (PIC) ranged from 0.0968–0.9310 (mean 0.3590), 0.0936–0.5085 (mean 0.3550) and 0.0879–0.3750 (mean 0.2807), respectively. Only 3 SNPs (Sob-016, Sob-050 and Sob-115) were significantly deviated from Hardy–Weinberg equilibrium (P < 0.05). Our study may help researchers better understand the genetic traits of S. obscurus,and provide reference for further protecting and reasonably utilizing its genetic resources.

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Acknowledgements

This study was supported by the Science and Technology Support Program of Guizhou Province in 2021 (General Items Program 2021-167: Collection, Development and Utilization of Germplasm Resources of Semilabeo obscurus, Department of Science and Technology of Guizhou Province), Support Program by Guizhou Academy of Agricultural Sciences in 2018 (Guidance Program 2018-04: Introduction, Domestication and Utilization of Semilabeo obscurus), The Breeding of Animals & Plants Research Projects of Guizhou Provincial Agricultural Committee in 2017 (2017-008: Introduction & Acclimatization and Conservation & Utilization of Semilabeo obscurus in Guizhou Province).

Funding

Funding was provided by Science and technology support program of Guizhou Province (2021-167).

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Author notes

  1. Shikai Li and Wei Wang have contributed equally to the manuscript.

Authors and Affiliations

  1. Fisheries Research Institute of Guizhou Academy of Agricultural Sciences, No. 2448 Huaxi Avenue, Guiyang, 550025, Guizhou, China

    Shikai Li, Wei Wang, Mingju Yang, Fujiang Huang, Xue Wang, Sheng Zeng, Yan Xiang & Lu Zhou

  2. The Engineering and Technology Center of Special Fisheries, Guizhou, No. 2448 Huaxi Avenue, Guiyang, 550025, Guizhou, China

    Shikai Li, Wei Wang, Mingju Yang, Fujiang Huang, Xue Wang, Sheng Zeng, Yan Xiang & Lu Zhou

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  1. Shikai Li
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  2. Wei Wang
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  3. Mingju Yang
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  7. Yan Xiang
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  8. Lu Zhou
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Correspondence to Shikai Li.

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Li, S., Wang, W., Yang, M. et al. Development and characterization of 123 SNPs in an endangered fish Semilabeo obscurus. Conservation Genet Resour 14, 257–261 (2022). https://doi.org/10.1007/s12686-022-01271-6

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  • DOI: https://doi.org/10.1007/s12686-022-01271-6

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