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Development and characterization of microsatellite loci in the sub-canopy tree Chengiopanax sciadophylloides (Araliaceae) using next-generation sequencing technology

  • Microsatellite Letters
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Abstract

Chengiopanax sciadophylloides is a deciduous broad-leaved sub-canopy tree species that is endemic to Japan and widely distributed in its temperate forests. Recent analyses indicate that climate change will reduce its distributional range and cause habitat fragmentation, leading to a loss of genetic variation. Here we describe the development of 15 microsatellite markers for this species using next-generation sequencing techniques. A total of 44 individuals from two populations were genotyped at each marker. The number of alleles per locus ranged from two to 13 with a mean of 4.8, and the expected heterozygosity ranged from 0.215 to 0.895 with a mean of 0.560. None of the loci exhibited linkage disequilibrium or any deviation from Hardy–Weinberg equilibrium. Because these 15 microsatellite markers exhibit high degrees of genetic variation, they should be useful tools for studying the conservation genetics of C. sciadophylloides.

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research (25850101) from the Japanese Society for the Promotion of Science.

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Authors and Affiliations

  1. Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, 036-8561, Japan

    Takeshi Torimaru, Takumi Okayama & Yutaka Kumagai

  2. Department of Environmental Science and Technology, Graduate School of Bioresources, Mie University, Tsu, 514-8507, Japan

    Takeshi Torimaru

  3. Department of Applied Biosciences, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, 036-8561, Japan

    Shinji Akada

Authors
  1. Takeshi Torimaru
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  2. Takumi Okayama
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  3. Yutaka Kumagai
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  4. Shinji Akada
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Corresponding author

Correspondence to Takeshi Torimaru.

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Torimaru, T., Okayama, T., Kumagai, Y. et al. Development and characterization of microsatellite loci in the sub-canopy tree Chengiopanax sciadophylloides (Araliaceae) using next-generation sequencing technology. Conservation Genet Resour 6, 799–801 (2014). https://doi.org/10.1007/s12686-014-0223-7

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  • DOI: https://doi.org/10.1007/s12686-014-0223-7

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