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Isolation and characterization of nineteen novel polymorphic microsatellite loci for the northern snakehead Channa argus

  • Microsatellite Letters
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Abstract

Channa argus is one of the most important economic fish species in China. However, there is limited genetic information on its population structure and genetic diversity. Nineteen novel polymorphic microsatellite loci were isolated and characterized in 42 individuals from one natural population collected from the Poyang Lake in Jiangxi Province. The number of alleles per locus ranged from 5 to 18 with an average of 11.16. The observed and expected heterozygosities ranged from 0.214 to 1.000 and from 0.630 to 0.923, respectively. The average polymorphic information content value was 0.814. Among these polymorphic microsatellites, eleven loci conformed to Hardy–Weinberg equilibrium. These microsatellite loci are currently being used to assess the genetic diversity and population structure of C. argus and will contribute to the effective conservation and rational utilization of genetic resources of this species.

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Acknowledgments

This work was supported by the National Key Technology R&D Program (Grant No. 2012BAD26B03) and by the Scientific Research Foundation for the Introduction of High-level Talents, Huazhong Agricultural University.

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Authors and Affiliations

  1. Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Ruo-Jin Yan, Kai-Jian Wei, Xiang-Zhao Guo, Gui-Rong Zhang, Jonathan P. A. Gardner & Rui-Bin Yang

  2. Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, People’s Republic of China

    Ruo-Jin Yan, Kai-Jian Wei, Xiang-Zhao Guo, Gui-Rong Zhang, Jonathan P. A. Gardner & Rui-Bin Yang

  3. School of Biological Sciences, Victoria University of Wellington, P. O. Box 600, Wellington, 6140, New Zealand

    Jonathan P. A. Gardner

  4. Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, People’s Republic of China

    Kun-Ci Chen

Authors
  1. Ruo-Jin Yan
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  2. Kai-Jian Wei
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  3. Xiang-Zhao Guo
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  4. Gui-Rong Zhang
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  5. Jonathan P. A. Gardner
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  6. Rui-Bin Yang
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  7. Kun-Ci Chen
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Correspondence to Gui-Rong Zhang.

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Yan, RJ., Wei, KJ., Guo, XZ. et al. Isolation and characterization of nineteen novel polymorphic microsatellite loci for the northern snakehead Channa argus . Conservation Genet Resour 6, 621–623 (2014). https://doi.org/10.1007/s12686-014-0155-2

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  • DOI: https://doi.org/10.1007/s12686-014-0155-2

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