Skip to main content

Advertisement

Log in

Novel microsatellite loci to investigate population structure in the endangered gray bat (Myotis grisescens)

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

We isolated and characterized 16 polymorphic microsatellite loci in the endangered gray bat (Myotis grisescens). This set of loci will be used to investigate patterns of genetic structure in hibernacula and maternity colonies of the gray bat across their range, ultimately combining multiple lines of evidence (mtDNA data, stable isotope signatures, etc.) to develop a spatial network for the species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Boutin-Ganache I, Raposo M, Raymond M, Deschepper CF (2001) M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods. Biotechniques 31:24–28

    PubMed  CAS  Google Scholar 

  • Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567

    Article  PubMed  Google Scholar 

  • Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94

    Article  PubMed  CAS  Google Scholar 

  • Harvey MJ, Altenbach JS, Best TL (1999) Bats of the United States. Arkansas Game & Fish Commission, Little Rock

    Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225

    Article  Google Scholar 

  • Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press, Totowa

    Google Scholar 

  • Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments: a poor man’s approach to genotyping for research and high-throughput diagnostics. Nat Biotechnol 18:233–234

    Article  PubMed  CAS  Google Scholar 

  • Tuttle MD (1979) Status, cause of decline, and management of endangered gray bats. J Wildl Manag 43:1–17

    Article  Google Scholar 

  • U.S. Fish and Wildlife Service (2012) January 17. North American bat death toll exceeds 5.5 million from white-nose syndrome [News Release]

  • U.S. Fish and Wildlife Service (2012) May 29. White-nose Syndrome Confirmed in Federally Endangered Gray Bats [News Release]

Download references

Acknowledgments

The authors thank N. Beane, E. Britzke, and M. Whitby for assistance with specimen collection, and C. Edwards for providing software assistance and manuscript review. The study described and the resulting data presented herein were obtained from research conducted under the U.S. Army Environmental Quality Technology Program by the U.S. Army Engineer Research and Development Center.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Denise L. Lindsay.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lindsay, D.L., Jung, M.G. & Lance, R.F. Novel microsatellite loci to investigate population structure in the endangered gray bat (Myotis grisescens). Conservation Genet Resour 5, 1055–1057 (2013). https://doi.org/10.1007/s12686-013-9969-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-013-9969-6

Keywords

Navigation