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Development of polysomic microsatellite markers for characterization of population structuring and phylogeography in the shortnose sturgeon (Acipenser brevirostrum)

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Abstract

Shortnose sturgeon Acipenser brevirostrum is an endangered polyploid fish species for which no nuclear DNA markers previously existed. To address this need, 86 polysomic loci were developed and characterized in 20 A. brevirostrum from five river systems and eight members (parents and six progeny) of a captive-bred family. All markers proved to be polymorphic, polysomic, and demonstrated direct inheritance when tested in a captive family. Eleven loci were included in a range-wide survey of 561 fish sampled from 17 geographic collections. Allelic diversity at these markers ranged from 7 to 24 alleles/locus and averaged 16.5 alleles/locus; sufficient diversity to produce unique multilocus genotypes. In the range-wide survey, a Mantel comparison of an ecological (1-Jaccard’s) and genetic (ΦPT; an analog to FST) distance metrics, identified a strong positive correlation (r = 0.98, P < 0.0001); suggesting ΦPT represents a viable metric for assessing genetic relatedness using this class of marker.

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Acknowledgments

The National Capital Region (NCR) of the U.S. National Park Service (NPS), and the U.S. Geological Survey’s (USGS) Leetown Science Center provided principal funding for this project through the National Resources Preservation Program. The Northeast Regional Office, Protected Resources Division, NOAA Fisheries, Department of Commerce also provided partial funding. The authors wish to acknowledge the efforts of Diane Pavek (NPS-NCR) for being the driving force behind this research. Douglas Peterson and Robert De Vries, University of Georgia, provided the Altamaha River tissue samples. Boyd Kynard (Retired), U.S. Geological Survey’s S.O. Conte Anadromous Fish Research Laboratory, provided sturgeon tissues from the Connecticut River. The U.S. Fish and Wildlife Service’s Bear’s Bluff National Fish Hatchery provided tissue samples of the parents and progeny from each of seven captive-bred A. brevirostrum families. These samples were instrumental in determining the inheritance of the polysomic markers. Paul Bailey and Mike Eackles (USGS Leetown Science Center) kindly provided laboratory assistance for this project. Use of trade, product, or firm names does not imply endorsement by the U.S. Government.

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Authors and Affiliations

  1. U.S. Geological Survey, Leetown Science Center, Fish Health Branch, 11649 Leetown Road, Kearneysville, WV, 25430, USA

    Anne P. Henderson

  2. U.S. Geological Survey, Leetown Science Center, Aquatic Ecology Branch, 11649 Leetown Road, Kearneysville, WV, 25430, USA

    Tim L. King

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  1. Anne P. Henderson
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  2. Tim L. King
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Correspondence to Anne P. Henderson.

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Henderson, A.P., King, T.L. Development of polysomic microsatellite markers for characterization of population structuring and phylogeography in the shortnose sturgeon (Acipenser brevirostrum). Conservation Genet Resour 4, 853–859 (2012). https://doi.org/10.1007/s12686-012-9658-x

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  • DOI: https://doi.org/10.1007/s12686-012-9658-x

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