Abstract
The red-bellied piranha belongs to the Pygocentrus genus, and is a representative of the Serrasalmidae family. The P. nattereri is the most abundant species in the floodplain of the Solimões-Amazonas river system. In the present work, 11 microsatellite loci were developed and used to investigate the genetic variation on 35 wild individuals. The number of alleles per locus ranged from 3 to 15, with an average of 8.2. The observed and expected heterozygosity values varied from 0.300 to 0.824 (mean 0.559) and 0.261 to 0.779 (mean 0.571), respectively. Only one loci (PN11) deviated significantly from Hardy–Weinberg equilibrium after Bonferroni correction. No significant linkage disequilibrium was detected. These polymorphic markers should be useful tools for assessing population genetics of P. nattereri, an overfished species in the Amazon varzea lakes that is most appreciated as a food item.
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References
Belkhir KP, Borsa P, Chikhi L, Raufaste N, Bonhomme F (2004) GENETIX 4.05, logiciel sous Windows TM pour la génétique des populations. Laboratoire du Génome et Populations, Interactions, CNRS UMR 5171. Université de Montpellier II, Montpellie
Billotte N, Lagoda PJR, Risterucci AM, Baurens FC (1999) Microsatellite-enriched libraries: applied methodology for the development of SSR markers in tropical crops. Fruits 54:277–288
Bittencourt MM (1994) Aspectos da demografia e do ciclo de vida de Pygocentrus nattereri Kner, 1860 em um lago de várzea da Amazônia Central (Lago do Rei, Ilha do Careiro). 205f. (Tese de Doutorado em Biologia de água Doce e Pesca Interior), 1994. Instituto Nacional de Pesquisas da Amazônia, Universidade Federal do Amazonas, Manaus, Amazonas, Brasil
Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (1991) “Touchdown” PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19(14):4008
Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3.2). Available from http://www.unil.ch/izea/softwares/fstat.html (Updated from: Goudet J (1995) FSTAT (version 1.2): a computer program to calculate F-statistics. J Hered 86:485–486
Hubert N, Renno JF (2006) Historical biogeography of South American freshwater fishes. J Biogeogr 33:1414–1436
Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106
Latini AO, Petrere M Jr (2004) Reduction of a native fish fauna by alien species: an example from Brazilian freshwater tropical lakes. Fish Manag Ecol 11:71–79
Lowe-McConnell RH (1987) Ecological studies in tropical fish communities. Cambridge University Press, Cambridge
Machado-Alison A, Fink W (1995) Sinopsis de las especies de la subfamilia Serrasalminae presentes en la cuenca Del Orinoco. Claves, Diagnosis e Ilustraciones. Lito Color RI, Caracas
Merona B, Bittencourt MM (1993) Les peuplements de poissons du “Lago do Rei, um lac d’inodation d’Amazonie centrale: description générale. Amazoniana 12(3/4):415–441
Mirande JM (2010) Philogeny of the Characidae (Teleostei: Characiformes): from characters to taxonomy. Neotrop Ichthyol 8(3):385–568
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Saint-Paul U, Zuanon J (2000) Fish communities in central Amazonian white and blackwater floodplains. Environ Biol Fish 53:235–250
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, New York
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nature Biotech 18:233–234
Silva ECS, Araújo-Lima CARM (2003) Influência do tipo de alimentação e da temperatura de evacuação gástrica da piranha caju (Pygocentrus nattereri) em condições experimentais. Acta Amazônica 33(1):145–156
van Oosterhout C, Hutchinson WF, Wills DP, Shipley P (2004) Microchecker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Acknowledgments
The authors are grateful to the PIATAM Project for providing the samples collected from a wild population in lakes along Solimões river between the cities of Coari and Manaus. The authors would like to thanks Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Amazonas (FAPEAM) and Conselho Nacional de Pesquisa (CNPq) for financial supports. CHAS is the recipient of a PhD fellowship from CNPq. We are thankful to Marcos Prado Lima, for construction the bank of microsatellite to Pygocentrus nattereri. The Program for Academic Cooperation PROCAD between INPA/UNICAMP/UFRGS was supported by CAPES (no.023/2006/CAPES). VMFAV are the recipient of research fellowship from CNPq. This work is part of INCT ADAPTA, coordinated by Adalberto Luis Val.
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Nascimento, P.R.M., Santos, C.H.A., Sousa, C.F.S. et al. Isolation and development microsatellite markers in the Pygocentrus nattereri (Kner, 1858) (Characiformes, Serrasalminae), an important freshwater fish in the Amazon. Conservation Genet Resour 4, 271–274 (2012). https://doi.org/10.1007/s12686-011-9522-4
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DOI: https://doi.org/10.1007/s12686-011-9522-4