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Development of microsatellite loci for Syzygium paniculatum (Myrtaceae), a rare polyembryonic rainforest tree

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Abstract

Fourteen novel microsatellite loci were developed for the rare polyembryonic and potentially polyploid rainforest tree Syzygium paniculatum Gaertn. Markers were characterized using 40 individuals from populations along the NSW coast. The mean number of alleles amplified per locus was three (range 1–7, H E = 0.54 and H′ = 0.85). Low allelic variation may be explained by the polyembryonic nature of S. paniculatum seeds. These markers provide the opportunity to use nuclear microsatellite markers to study the population genetic structure and breeding system in S. paniculatum and related species.

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References

  • Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222

    Article  CAS  PubMed  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Nic Lughadha E, Proença C (1996) A survey of the reproductive biology of the Myrtoideae (Myrtaceae). Ann Mo Bot Gard 83:480–503

    Article  Google Scholar 

  • Roy SK (1961) Embryology of Eugenia fruticosa. Proc Natl Acad Sci India B 31(1):80–87

    Google Scholar 

  • Rozen S, Skalesky H (2000) PRIMER 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386

    Google Scholar 

  • Sahai R, Roy SK (1962) Polyembryony in Eugenia heyneana Duthie. Sci Cult 28:37–38

    Google Scholar 

  • Schuelke M (2000) An economic method for fluorescent labelling of PCR fragments. Nat Biotechnol 18:233–234

    Article  CAS  PubMed  Google Scholar 

  • Van Puyvelde K, Van Geert A, Triest L (2010) ATETRA, a new software program to analyse tetraploid microsatellite data: comparison with TETRA and TETRASAT. Mol Ecol Res 10(2):331–334

    Article  Google Scholar 

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Acknowledgments

We would like to thank the Australian Flora Foundation for funding, Hannah McPherson and William Sherwin for technical input and The Ramaciotti Centre for Gene Function Analysis (University of New South Wales, Australia) for performing sequencing.

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Authors and Affiliations

  1. National Herbarium of New South Wales, Botanic Gardens Trust, Mrs Macquaries Rd, Sydney, NSW, 2000, Australia

    Katie A. G. Thurlby, Carolyn Connelly, Peter G. Wilson & Maurizio Rossetto

  2. School of Biological, Earth, and Environmental Sciences, University of New South Wales, Sydney, NSW, 2052, Australia

    Katie A. G. Thurlby

  3. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia

    Katie A. G. Thurlby

Authors
  1. Katie A. G. Thurlby
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  2. Carolyn Connelly
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  3. Peter G. Wilson
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  4. Maurizio Rossetto
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Corresponding author

Correspondence to Katie A. G. Thurlby.

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Thurlby, K.A.G., Connelly, C., Wilson, P.G. et al. Development of microsatellite loci for Syzygium paniculatum (Myrtaceae), a rare polyembryonic rainforest tree. Conservation Genet Resour 3, 205–208 (2011). https://doi.org/10.1007/s12686-010-9323-1

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  • DOI: https://doi.org/10.1007/s12686-010-9323-1

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