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Isolation and characterization of eight novel microsatellite loci in the double-crested cormorant (Phalacrocorax auritus)

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Abstract

We describe the isolation and characterization of eight microsatellite loci from the double-crested cormorant (Phalacrocorax auritus). Genetic variability was assessed using 60 individuals from three populations. All loci were variable with the number of alleles ranging from two to 17 per locus, and observed heterozygosity varying from 0.05 to 0.89. No loci showed signs of linkage disequilibrium and all loci conformed to Hardy–Weinberg equilibrium frequencies. Further, all loci amplified and were polymorphic in two related Phalacrocorax species. These loci should prove useful for population genetic studies of the double-crested cormorant and other pelecaniform species.

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Acknowledgments

We thank all of the individuals who collected cormorant samples. We are grateful to Daniel Roby for support of this research and for providing comments on earlier drafts of this paper. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the US Government.

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Authors and Affiliations

  1. U.S. Geological Survey Forest and Rangeland Ecosystem Science Center, 3200 SW Jefferson Way, Corvallis, OR, 97331, USA

    Dacey M. Mercer, Susan M. Haig & Thomas D. Mullins

  2. Oregon State University, Corvallis, OR, USA

    Dacey M. Mercer

Authors
  1. Dacey M. Mercer
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  2. Susan M. Haig
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  3. Thomas D. Mullins
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Correspondence to Dacey M. Mercer.

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Mercer, D.M., Haig, S.M. & Mullins, T.D. Isolation and characterization of eight novel microsatellite loci in the double-crested cormorant (Phalacrocorax auritus). Conservation Genet Resour 2, 119–121 (2010). https://doi.org/10.1007/s12686-010-9178-5

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  • DOI: https://doi.org/10.1007/s12686-010-9178-5

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