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Electrochemical Biosensors for Soluble Epidermal Growth Factor Receptor Detection

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Abstract

Simple detection of oncomarkers is of paramount importance in cancer diagnosis. Screen-printed carbon electrodes (SPCEs) were modified by streptavidin, which served as a suitable platform for aptamer molecule binding. The electrochemical conditions for soluble epidermal growth factor receptor (EGFR) sensing were studied and optimized. Developed sensors display a wide linear range from 10 to 500 ng/ml and a low limit of detection of 1.19 ng/ml. SPCEs modified by streptavidin could be used not only as an amperometric sensor but also as an impedimetric one. Moreover, SPCEs were successfully tested for soluble EGFR detection in blood serum samples. This platform presents a simple solution for the application of aptamers in electrochemical testing without the multistep working electrode modification processes.

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Funding

This research was funded by Slovak Research and Development Agency, grant number APVV-PP-COVID-20–0036.

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Authors and Affiliations

  1. Department of Physical Chemistry, Pavol Jozef Šafárik University in Košice, Moyzesova 11, 040 01, Košice, Slovak Republic

    Jana Shepa, Ivana Šišoláková & Renáta Oriňaková

  2. Institute of Biology and Ecology, Pavol Jozef Šafárik University in Košice, Moyzesova 11, 040 01, Košice, Slovak Republic

    Martin Panigaj & Dominika Bilá

  3. Department of Infectology and Travel Medicine, Pavol Jozef Šafárik University in Košice, Moyzesova 11, 040 01, Košice, Slovak Republic

    Pavol Jarčuška

Authors
  1. Jana Shepa
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  2. Ivana Šišoláková
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  3. Martin Panigaj
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  4. Dominika Bilá
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  5. Pavol Jarčuška
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  6. Renáta Oriňaková
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Contributions

I. Š. and J. S. conceived of the presented idea. J. S. developed the theory and wrote the manuscript with support from I. Š. and M. P. I. Š. carried out the electrochemical experiments and M. P. and D. B. carried out the aptamer development. P. J. and R. O. supervised the work with the purchased blood serum realized under specific conditions. Both R. O. and P. J. authors contributed to the final version of the manuscript.

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Correspondence to Ivana Šišoláková.

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Shepa, J., Šišoláková, I., Panigaj, M. et al. Electrochemical Biosensors for Soluble Epidermal Growth Factor Receptor Detection. Electrocatalysis 13, 513–523 (2022). https://doi.org/10.1007/s12678-022-00740-8

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  • DOI: https://doi.org/10.1007/s12678-022-00740-8

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