Abstract
Postnatal islets of Langerhans retain cellular elements that have a several stem cell markers. It can be supposed that islet cells are not a static population and they can give rise to the new endocrinocytes. The aim of our work was to study the proliferative capacity of pancreatic islet cells and their subsequent differentiation in the late prenatal, early postnatal, and adult periods of human ontogenesis. A study of the late perinatal development of the human pancreas was performed on a sample resulting of legal medical abortion with the voluntary consent of the mother (30 weeks gestation), autopsy of the infant pancreas (from 17 days to 2 months after the birth) and adult pancreas (38–66 years old). Pancreas paraffin sections were stained immunohistochemically with commercial antibodies to insulin, Ki-67 (marker of proliferation), glucagon, and C-kit (stem and progenitor cell marker). It was found that proliferating cells remain in human pancreatic islets up to at least 2 months after the birth, the number of which can be up to 3.5% of islet cells. Some of these cells can synthesize glucagon. Based on the obtained results, we suppose that the cells of Langerhans islet are not a constant or static cells population. After the birth, there are cells in the islet which are capable to proliferate and differentiate. Because endocrinocyte differentiation into β-cells goes through the stage of glucagon-producing cells, it is possible that the origin of diabetes mellitus type I may be associated with the disturbance of proliferation and differentiation of the islet of Langerhans cells and a population imbalance between already differentiated α and β-cells.
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References
Bonner-Weir, S. (2001). Beta-cell turnover: Its assessment and implications. J Diabets, 50, 20–24. https://doi.org/10.2337/diabetes.50.2007.s20
Scaglia, L., Cahill, C. J., Finegood, D. T., & Bonner-Weir, S. (1997). Apoptosis participates in the remodeling of the endocrine pancreas in the neonatal rat. Endocrinology, 138, 1736–1741. https://doi.org/10.1210/endo.138.4.5069
Montana, E., Bonner-Weir, S., & Weir, G. C. (1994). Transplanted beta cell response to increased metabolic demand. The Journal of Clinical Investigation, 93, 1577–1582. https://doi.org/10.1172/jci117137
Bouwens, L., & Pipeleers, D. G. (1998). Extra-insular beta cells associated with ductules are frequent in adult human pancreas. Diabetologia, 41, 629–633. https://doi.org/10.1007/s001250050960
Bouwens, L., Lu, W. G., & De Krijger, R. (1997). Proliferation and differentiation in the human fetal endocrine pancreas. Diabetologia, 40(4), 398–404. https://doi.org/10.1007/s001250050693
Kaligin, M. S., Pliushkina, A. S., Titova, A. A., Titova, M. A., Gumerova, A. A., & Kiiasov, A. P. (2015). C-kit expression as a feature of functional differentiation of progenitor cells. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6(4), 2175–2183.
Kaligin, M., Andreeva, D., Titova, A., Titova, M., Gumerova, A., & Kiyasov, A. (2018). Target cells for stem cell factor in the adult Islets of Langerhans, simultaneously synthesizing glucagon and insulin. BioNanoScience, 8(1), 296–298. https://doi.org/10.1007/S12668-017-0444-9
Ye, L., Robertson, M. A., Hesselson, D., Stainier, D. Y. R., & Anderson, R. M. (2015). Glucagon is essential for alpha cell transdifferentiation and beta cell neogenesis. Development, 142, 1407–1417. https://doi.org/10.1242/dev.117911
Li, J., Quirt, J., Do, H. Q., Lyte, K., Fellows, F., Goodyer, C. G., & Wang, R. (2007). Expression of C-kit receptor tyrosine kinase and effect on beta-cell development in the human fetal pancreas. American journal of physiology. Endocrinology and metabolism, 293(2), 475–483. https://doi.org/10.1152/ajpendo.00172.2007
Olsson, R., Maxhuni, A., & Carlsson, P. O. (2006). Revascularization of transplanted pancreatic islets following culture with stimulators of angiogenesis. Transplantation, 82, 340–347. https://doi.org/10.1097/01.tp.0000229418.60236.87
Paltsev, M. A., Kaktursky, L. V., & Zayratyants, O. V. (2014). Pathological anatomy: national guide. Moscow: GEOTAR-Media.
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This paper has been supported by the Kazan Federal University Strategic Academic Leadership Program.
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Kaligin, M., Valijonov, K., Pliushkina, A. et al. Dividing Cells in Islets of Langerhans Can produce Glucagon. BioNanoSci. 12, 1388–1393 (2022). https://doi.org/10.1007/s12668-022-00989-9
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DOI: https://doi.org/10.1007/s12668-022-00989-9