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Evaluation of Different Oxidative Stress Parameters and Apoptosis in Human Cervical Cancer Cells Exposed to Rod and Spherical Shaped Zinc Oxide Nanoparticles

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A Correction to this article was published on 20 August 2019

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Abstract

Zinc oxide nanoparticles are presently being used in cosmetics, paints, biosensors, and medical applications. However, zinc oxide nanoparticles are broadly used in the cosmetic industry for many years; recently, they have been explored for use in cancer therapy. This study was intended to comprehend the mechanism of the differential cytotoxicity of rod and spherical shaped zinc oxide nanoparticles to cervical cancer cells HeLa and SiHa. The role of ion dissolution in the toxicity of zinc oxide nanoparticles was also investigated. Zinc oxide nanorod induced significantly higher oxidative stress, reactive oxygen species compared to spherical shaped zinc oxide nanoparticles. Additionally, rod shaped nanoparticles activated the NF-κB signaling pathway. Besides, zinc oxide nanorod also induced mitochondrial membrane damage leading to apoptosis as observed by PARP cleavage and reduction of Phospho-Bad. In conclusion, zinc oxide nanorod, after proper tailoring, may have potential to use as chemotherapeutic agents against cervical cancer.

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Change history

  • 20 August 2019

    The original article unfortunately contains minor errors in Fig. 1B.

  • 20 August 2019

    The original article unfortunately contains minor errors in Fig. 1B.

Abbreviations

NPs:

Nanoparticles

ZnO NPs:

Zinc oxide nanoparticles

ROS:

Reactive oxygen species

MMP:

Mitochondrial membrane potential

TEM:

Transmission electron microscope

FE-SEM:

Field-emission scanning electron microscope

DLS:

Dynamic light scattering

PDI:

Polydispersity index

XRD:

X-ray diffraction

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide

DCFDA:

2,7-Dichlorofluorescein di-acetate

MDA:

Malondialdehyde

LPO:

Lipid peroxidation

GSH:

Glutathione

PC:

Protein carbonyl

SOD:

Superoxide dismutase

CAT:

Catalase

LDH:

Lactate dehydrogenase

JC-1 dye:

5,5′,6,6′-Tetrachloro-1,1′,3,3-tetraethylbenzimidazolcarbocyanine iodide

NF-κB:

Nuclear factor-kappa beta

PARP:

Poly ADP ribose polymerase

ARS:

Alizarin Red S

OD:

Optical density.

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Acknowledgments

This work was financially supported by UPE (Phase II), DST-PURSE program and State Government Fellowship scheme of Jadavpur University.

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  1. Parimal Karmakar

    Present address: Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S.C. Mullick Road, Kolkata, 700 032, West Bengal, India

Authors and Affiliations

  1. Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S.C. Mullick Road, Kolkata, 700 032, West Bengal, India

    Debalina Bhattacharya & Aritra Bhattacharyya

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  1. Debalina Bhattacharya
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Bhattacharya, D., Bhattacharyya, A. & Karmakar, P. Evaluation of Different Oxidative Stress Parameters and Apoptosis in Human Cervical Cancer Cells Exposed to Rod and Spherical Shaped Zinc Oxide Nanoparticles. BioNanoSci. 6, 1–14 (2016). https://doi.org/10.1007/s12668-015-0186-5

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