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Silver Nanoparticles Nanocarriers, Synthesis and Toxic Effect on Cervical Cancer Cell Lines

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Abstract

Silver nanoparticles (AgNPs) have been suggested as a potential tool for the anticancer treatment, but their pharmacological effect has not been tested in vivo because of their high reactivity and adverse effects. In this study, we developed AgNPs of 5 ± 2 nm of diameter through the chemical reduction of silver nitrate (AgNO3) with dextrose, employing gelatin as a surfactant agent, and encapsulated them in nanocarriers with a biocompatible surface based on polyethylene glycol through a modification of the emulsion solvent-evaporation technique. AgNPs nanocarriers (AgNPs-NT) showed diameters between 30 nm and 120 nm in dry samples and from 40 nm to 250 nm in aqueous solution. Different doses of free and encapsulated AgNPs were evaluated in vitro on the cervical cancer-derived cell lines HeLa and CaSki, by flow cytometry studies at 24 h, employing propidium iodide (PI) and carboxyfluorescein diacetate succinimidyl ester (CFSE) as reporter molecules. Both free and encapsulated AgNPs were toxic for both cell lines, inducing important decrements on the cell viability compared with cisplatin (PC, 0.250 mM) and the negative control (NCtrl) comprising only the cell culture media, the vehicle of the AgNPs (0.360 mM), and the vehicle of the AgNPs-NT (1.089 mM). In both cases (HeLa and CaSki cells), AgNPs-NT seem to be less effective than free AgNPs, but this AgNPs-NT had a biocompatible surface, adequate size, and high toxicity observed in vitro.

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Acknowledgments

Studies were developed with the economic support of the Secretaría de Investigación y Posgrado–IPN Mexico and the scholarships for Ms. Casañas from COFAA–IPN and the Fund Project of Basic Science No. 154942 of Consejo Nacional de Ciencia y Tecnología (CONACYT) Mexico.

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Authors and Affiliations

  1. Laboratorio de Biomateriales, Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, Instituto Politécnico Nacional, México, D.F., 11500, México

    Rocio Casañas Pimentel, Eduardo San Martín Martínez & Quetzaliztli G. Alvarado Palacios

  2. Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, México, D.F., 06720, México

    Alberto Monroy García

  3. Posgrado en Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, México, D.F., 07320, México

    Consuelo Gómez-García

Authors
  1. Rocio Casañas Pimentel
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  2. Eduardo San Martín Martínez
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  3. Alberto Monroy García
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  4. Consuelo Gómez-García
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  5. Quetzaliztli G. Alvarado Palacios
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Correspondence to Eduardo San Martín Martínez.

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Casañas Pimentel, R., San Martín Martínez, E., Monroy García, A. et al. Silver Nanoparticles Nanocarriers, Synthesis and Toxic Effect on Cervical Cancer Cell Lines. BioNanoSci. 3, 198–207 (2013). https://doi.org/10.1007/s12668-013-0085-6

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  • DOI: https://doi.org/10.1007/s12668-013-0085-6

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