Abstract
Laboratory exposure experiments were performed on the marine coastal zooplankton species Pseudodiaptomus marinus—a keystone species of vast Atlantic and Pacific marine ecosystems, tracing the accumulation of three polycyclic aromatic hydrocarbons (PAHs): phenanthrene, anthracene and pyrene. The experiment was designed to study the bioconcentration of PAHs in phytoplankton (Rhodomonas baltica) and accumulation in copepods (P. marinus), through two different pathways: food and water uptake. For this purpose, water and organism subsamples were collected, ASE (accelerated solvent extraction) extracted, and analyzed for PAHs by GC/MS technique. As a result, experimental bioconcentration (BCF) and bioaccumulation (BAF) factors which showed a significant correlation with the octanol–water partition coefficient (K ow) were presented for P. marinus. The active feeding route of exposure showed no significant differences versus passive uptake, highlighting the importance of dietary exposure as a major pathway for POPs accumulation in zooplankton; in particular for those which cannot be metabolized.
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Acknowledgments
Our sincere gratitude to Dr. Sopheak Net and David Dumoulin for their invaluable support throughout the research phase in France. We also thanks gratefully to the Erasmus Mundus mobility program staff (UE). We thank Olivier Glippa and Damien Bocahu for the discussions and help during these experiments. Special thanks to past and present members of Sami Souissi’s team for their contribution in maintaining continuously strains and mass culture of several species of copepods, including P. marinus used in this study. We particularly thank the LOG COPEFISH team for their help in keeping P. marinus cultures in the laboratory since 2011 in the framework of the COPEFISH project (Young Researcher (A.S.). Emerging Project of the Nord-Pas de Calais Regional Council). We thank Hans-U. Dahms (Kaohsiung) for critical remarks on an earlier version of the MS.
Financial support
This work was carried out with the financial support of Lille-1 University and its Interdisciplinary Institute IREPSE (S.Souissi and B. Oudanne), the National Council of Scientific and Technological Research of Argentina (CONICET) and the Argentinean Agency of Science and Technology Promotion (ANPYCT) through PICT 2010-1302 (granted to A. Arias).
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This article is part of a Topical Collection in Environmental Earth Sciences on “3RAGSU”, guest edited by Daniel Emilio Martinez.
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Arias, A.H., Souissi, A., Roussin, M. et al. Bioaccumulation of PAHs in marine zooplankton: an experimental study in the copepod Pseudodiaptomus marinus . Environ Earth Sci 75, 691 (2016). https://doi.org/10.1007/s12665-016-5472-1
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DOI: https://doi.org/10.1007/s12665-016-5472-1