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Absence of Mycobacterium avium ss paratuberculosis-specific IS900 sequence in intestinal biopsy tissues of Indian patients with Crohn’s disease

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Abstract

Background and Objective

The role of Mycobacterium avium ss paratuberculosis (MAP) in the etiopathology of Crohn’s disease (CD) remains controversial, because of conflicting reports demonstrating the presence of MAP-specific insertion sequence from intestinal biopsy tissues of patients clinically diagnosed for the disease. The present study was carried out to investigate the presence of MAP DNA in the intestinal tissues of CD patients to ascertain the relevance of MAP in Indian patients with CD.

Methods

Patients diagnosed as CD at our institute were recruited. Healthy individuals without inflammatory bowel disease served as controls. Mucosal biopsy specimens were collected from ileum and colon in duplicates and subjected to histopathological examination and polymerase chain reaction (PCR) amplification. Total DNA (81 CD patients, 85 healthy individuals) and total RNA (12 CD patients, 12 healthy individuals) isolated from tissue specimens was used for amplification of MAP-specific IS900 by nested PCR.

Results

MAP-specific IS900 DNA and RNA could not be detected by nested PCR in the intestinal tissues of any patient with CD.

Conclusion

Our results do not support the etiological role of MAP in the pathogenesis of CD in Indian patients.

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References

  1. Chiodini RJ. Crohn’s disease and the mycobacterioses: a review and comparison of two disease entities. Clin Microbiol Rev 1989;2:90–117.

    CAS  PubMed  Google Scholar 

  2. Cocito C, Gilot P, Coene M, et al. Paratuberculosis. Clin Microbiol Rev 1994;7:328–345.

    CAS  PubMed  Google Scholar 

  3. Chacon O, Bermudez LE, Barletta RG. Johne’s disease, inflammatory bowel disease and Mycobacterium paratuberculosis. Annu Rev Microbiol 2004;58:329–363.

    Article  CAS  PubMed  Google Scholar 

  4. Clarke CJ. The pathology and pathogenesis of paratuberculosis in ruminants and other species. J Comp Pathol 1997;116:217–221.

    Article  CAS  PubMed  Google Scholar 

  5. McClure HM, Chiodini RJ, Anderson DC, et al. Mycobacterium paratuberculosis infection in a colony of stumptail macaques (Macaca arctoides). J Infect Dis 1987;155:1011–1019.

    CAS  PubMed  Google Scholar 

  6. Green EP, Tizard ML, Moss MT, et al. Sequence and characteristics of IS900, an insertion element identified in a human Crohn’s disease isolate of Mycobacterium paratuberculosis. Nucleic Acids Res 1989;17:9063–9073.

    Article  CAS  PubMed  Google Scholar 

  7. Sanderson JD, Moss MT, Tizard ML, Hermon-Taylor J. Mycobacterium paratuberculosis DNA in Crohn’s disease tissue. Gut 1992;33:890–896.

    Article  CAS  PubMed  Google Scholar 

  8. Lisby G, Andersen J, Engbaek K, Binder V. Mycobacterium paratuberculosis in intestinal tissue from patients with Crohn’s disease demonstrated by a nested primer polymerase chain reaction. Scand J Gastroenterol 1994;29:923–929.

    Article  CAS  PubMed  Google Scholar 

  9. Burnham WR, Lennard-Jones JE, Stanford JL, Bird RJ. Mycobacteria as a possible cause of inflammatory bowel disease. Lancet 1978;2:693–696.

    Article  CAS  PubMed  Google Scholar 

  10. Collins MT, Lisby G, Moser C, et al. Results of multiple diagnostic tests for Mycobacterium avium subsp. paratuberculosis in patients with inflammatory bowel disease and in controls. J Clin Microbiol 2000;38:4373–4381.

    CAS  PubMed  Google Scholar 

  11. El-Zaatari FA, Osato MS, Graham DY. Etiology of Crohn’s disease: the role of Mycobacterium avium paratuberculosis. Trends Mol Med 2001;7:247–252.

    Article  CAS  PubMed  Google Scholar 

  12. Dell’Isola B, Poyart C, Goulet O, et al. Detection of Mycobacterium paratuberculosis by polymerase chain reaction in children with Crohn’s disease. J Infect Dis 1994;169:449–451.

    PubMed  Google Scholar 

  13. Fidler HM, Thurrell W, Johnson NM, Rook GA, McFadden JJ. Specific detection of Mycobacterium paratuberculosis DNA associated with granulomatous tissue in Crohn’s disease. Gut 1994;35:506–510.

    Article  CAS  PubMed  Google Scholar 

  14. Hermon-Taylor J. Mycobacterium paratuberculosis as a chronic enteric pathogen in humans. In: Chiodini RJ, Collins MT, Bassey EOE (eds). Proceedings of the Fourth International Colloquium on Paratuberculosis. Cambridge, UK. 1995; p. 388–394.

  15. McFadden J, Collins J, Beaman B, Arthur M, Getnick G. Mycobacteria in Crohn’s disease: DNA probes identify the wood pigeon strain of Mycobacterium avium and Mycobacterium paratuberculosis from human tissue. J Clin Microbiol 1992;30:3070–3073.

    CAS  PubMed  Google Scholar 

  16. Mishina D, Katsel P, Brown ST, Gilberts EC, Greenstein RJ. On the etiology of Crohn’s disease. Proc Natl Acad Sci USA 1996;93:9816–9820.

    Article  CAS  PubMed  Google Scholar 

  17. Moss MT, Sanderson JD, Tizard ML, et al. Polymerase chain reaction detection of Mycobacterium paratuberculosis and Mycobacterium avium subsp silvaticum in long term cultures from Crohn’s disease and control tissues. Gut 1992;33:1209–1213.

    Article  CAS  PubMed  Google Scholar 

  18. Murray A, Oliaro J, Schlup MM, Chadwick VS. Mycobacterium paratuberculosis and inflammatory bowel disease: frequency distribution in serial colonoscopic biopsies using the polymerase chain reaction. Microbios 1995;83:217–228.

    CAS  PubMed  Google Scholar 

  19. Schaefer WB, Lewis CW Jr. Effect of oleic acid on growth and cell structure of mycobacteria. J Bacteriol 1965;90:1438–1447.

    CAS  PubMed  Google Scholar 

  20. Stabel JR, Steadham EM, Bolin CA. Heat inactivation of Mycobacterium paratuberculosis in raw milk: are current pasteurization conditions effective? Appl Environ Microbiol 1997;63:4975–4977.

    CAS  PubMed  Google Scholar 

  21. Suenaga K, Yokoyama Y, Okazaki K, Yamamoto Y. Mycobacteria in the intestine of Japanese patients with inflammatory bowel disease. Am J Gastroenterol 1995;90:76–80.

    CAS  PubMed  Google Scholar 

  22. Chamberlin W, Graham DY, Hulten K, et al. Review article: Mycobacterium avium subsp. paratuberculosis as one cause of Crohn’s disease. Aliment Pharmacol Ther 2001;15:337–346.

    Article  CAS  PubMed  Google Scholar 

  23. Gibson J, Riggio M, McCreary C, Lennon A, Toner M. Looking for Mycobacterium paratuberculosis DNA by polymerase chain reaction (PCR) in orofacial granulomatosis (OFG) and oral Crohn’s disease tissue in an Irish population. Ir Med J 2000;93:218.

    CAS  PubMed  Google Scholar 

  24. Van Kruiningen HJ. Lack of support for a common etiology in Johne’s disease of animals and Crohn’s disease in humans. Inflamm Bowel Dis 1999;5:183–191.

    Article  PubMed  Google Scholar 

  25. Quirke P. Antagonist. Mycobacterium avium subspecies paratuberculosis is a cause of Crohn’s disease. Gut 2001;49:757–760.

    Article  CAS  PubMed  Google Scholar 

  26. Frank TS, Cook SM. Analysis of paraffin sections of Crohn’s disease for Mycobacterium paratuberculosis using polymerase chain reaction. Mod Pathol 1996;9:32–35.

    CAS  PubMed  Google Scholar 

  27. Rowbotham DS, Mapstone NP, Trejdosiewicz LK, Howdle PD, Quirke P. Mycobacterium paratuberculosis DNA not detected in Crohn’s disease tissue by fluorescent polymerase chain reaction. Gut 1995;37:660–667.

    Article  CAS  PubMed  Google Scholar 

  28. Wu SW, Pao CC, Chan J, Yen TS. Lack of mycobacterial DNA in Crohn’s disease tissue. Lancet 1991;337:174–175.

    Article  CAS  PubMed  Google Scholar 

  29. Kanazawa K, Haga Y, Funakoshi O, Nakajima H, Munakata A, Yoshida Y. Absence of Mycobacterium paratuberculosis DNA in intestinal tissues from Crohn’s disease by nested polymerase chain reaction. J Gastroenterol 1999;34:200–206.

    Article  CAS  PubMed  Google Scholar 

  30. Sands BE. From Symptoms to diagnosis: clinical distinctions among various forms of intestinal inflammation. Gastroenterology 2004;126:1518–1532.

    Article  PubMed  Google Scholar 

  31. Naser SA, Ghobrial G, Romero C, Valentine JF. Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn’s disease. Lancet 2004;364:1039–1044.

    Article  PubMed  Google Scholar 

  32. Ellingson JL, Cheville JC, Brees D, Miller JM, Cheville NF. Absence of Mycobacterium avium subspecies paratuberculosis components from Crohn’s disease intestinal biopsy tissues. Clin Med Res 2003;1:217–226.

    Article  PubMed  Google Scholar 

  33. Singh AV, Singh SV, Makharia GK, Singh PK, Sohal JS. Presence and characterization of Mycobacterium avium subspecies paratuberculosis from clinical and suspected cases of Crohn’s disease and in the healthy human population in India. Int J Infect Dis 2008;12:190–197.

    Article  CAS  PubMed  Google Scholar 

  34. Suenaga K, Yokoyama Y, Nishimori I, et al. Serum antibodies to Mycobacterium paratuberculosis in patients with Crohn’s disease. Dig Dis Sci 1999;44:1202–1207.

    Article  CAS  PubMed  Google Scholar 

  35. Selby W, Pavli P, Cotty B, et al. Two-year combination therapy with clarithromycin, rifabutin and clofazimine for Crohn’s disease. Gastroenterology 2007;132:2313–2319.

    Article  CAS  PubMed  Google Scholar 

  36. Gao A, Odumeru J, Raymond M, Hendrick S, Duffield T, Mutharia L. Comparison of milk culture, direct and nested polymerase chain reaction (PCR) with fecal culture based on samples from dairy herds infected with Mycobacterium avium subsp. paratuberculosis. Can J Vet Res 2009;73:58–64.

    PubMed  Google Scholar 

  37. Parrish NM, Radcliff RP, Brey BJ, et al. Absence of Mycobacterium avium subsp. paratuberculosis in Crohn’s patients. Inflamm Bowel Dis 2008;15:558–565.

    Article  Google Scholar 

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Authors and Affiliations

  1. Asian Health Care Foundation, Somajiguda, Hyderabad, 500 082, India

    Mitnala Sasikala, Sanjeev Kumar Sharma, P. Reddy Balkumar & Rupa Banerjee

  2. Asian Institute of Gastroenterology, Somajiguda, Hyderabad, 500 082, India

    D. Nageshwar Reddy, Nitesh Pratap, Anuradha Sekaran & D. Bhaskara Reddy

Authors
  1. Mitnala Sasikala
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  2. D. Nageshwar Reddy
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  3. Nitesh Pratap
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  4. Sanjeev Kumar Sharma
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Correspondence to Mitnala Sasikala.

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Sasikala, M., Reddy, D.N., Pratap, N. et al. Absence of Mycobacterium avium ss paratuberculosis-specific IS900 sequence in intestinal biopsy tissues of Indian patients with Crohn’s disease. Indian J Gastroenterol 28, 169–174 (2009). https://doi.org/10.1007/s12664-009-0068-2

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  • DOI: https://doi.org/10.1007/s12664-009-0068-2

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