Abstract
Purpose
As the issue of sustainability gains importance in recent days, efforts to reduce or eliminate waste problems are gaining importance. The use of various industrial by-products is being investigated to produce edible packaging materials. In this study, red pepper pomace oil and protein were extracted and incorporated into corn starch film and their effects on the film properties were analyzed.
Method
Protein isolate was added to the films as 10–30% of the starch mass and oil as 15% of the solid mass of the film containing 10% protein isolate. The edible composite films characterized by physical, barrier, mechanical, antioxidant and antimicrobial properties and FTIR spectroscopy.
Results
Pomace oil addition increased the thickness. While pomace oil decreased water solubility, protein isolate increased it. Protein isolate and pomace oil did not alter swelling and water vapor permeability. The protein isolate decreased the L* value and the pomace oil decreased the a* value. Both protein isolate and pomace oil increased yellowness in the films. A low amount (10%) of protein isolates increased the transparency of films. The combined use of protein isolate and pomace oil decreased the elongation at break and increased the tensile strength of the films. Antimicrobial effect against Escherichia coli and antioxidant activity of oil containing film were observed.
Conclusion
The results suggest that valuable components can be obtained from red pepper pomace, and these components can be added to modify the characteristics of maize starch edible films.
Graphical Abstract
Similar content being viewed by others
Data Availability
The datasets generated during and/or analyzed during the current study are available on request from the authors.
References
Rhim, J.W., Hong, S.I., Ha, C.S.: Tensile, water vapor barrier and antimicrobial properties of PLA/nanoclay composite films. LWT-Food Sci. Technol. 42, 612–617 (2009)
Lee, J.H., Song, K.B.: Preparation of a porcine plasma protein composite film and its application. Biosci. Biotechnol. Biochem. 79, 68–73 (2015)
Condés, M.C., Añón, M.C., Mauri, A.N., Dufresne, A.: Amaranth protein films reinforced with maize starch nanocrystals. Food Hydrocoll. 47, 146–157 (2015)
Azevedo, V.M., Borges, S.V., Marconcini, J.M., Yoshida, M.I., Neto, A.R.S., Pereira, T.C., Pereira, C.F.G.: Effect of replacement of corn starch by whey protein isolate in biodegradable film blends obtained by extrusion. Carbohydr. Polym. 157, 971–980 (2017)
Müller, C.M., Laurindo, J.B., Yamashita, F.: Effect of cellulose fibers addition on the mechanical properties and water vapor barrier of starch-based films. Food Hydrocoll. 23, 1328–1333 (2009)
Su, B., Xie, F., Li, M., Corrigan, P.A., Yu, L., Li, X., Chen, L.: Extrusion processing of starch film. Int. J. Food Eng. 5, (2009)
Wu, Z., Huang, Y., Xiao, L., Lin, D., Yang, Y., Wang, H., Yang, Y., Wu, D., Chen, H., Zhang, Q., Qin, W., Pu, S.: Physical properties and structural characterization of starch/polyvinyl alcohol/graphene oxide composite films. Int. J. Biol. Macromol. 123, 569–575 (2019)
Bourtoom, T.: Edible films and coatings: Characteristics and properties. Int. Food Res. J. 15, 237–248 (2008)
Santana, R.F., Bonomo, R.C.F., Gandolfi, O.R.R., Rodrigues, L.B., Santos, L.S., dos Santos Pires, A.C., de Oliveira, C.P., da Costa Ilhéu Fontan, Veloso, R.: C.M.: Characterization of starch-based bioplastics from jackfruit seed plasticized with glycerol. J. Food Sci. Technol. 55, 278–286 (2018)
Seligra, P.G., Jaramillo, C.M., Famá, L., Goyanes, S.: Biodegradable and non-retrogradable eco-films based on starch–glycerol with citric acid as crosslinking agent. Carbohydr. Polym. 138, 66–74 (2016)
Primozic, M., Duchek, A., Nickerson, M., Ghosh, S.: Effect of lentil proteins isolate concentration on the formation, stability and rheological behavior of oil-in-water nanoemulsions. Food Chem. 237, 65–74 (2017)
Quanhong, L., Caili, F.: Application of response surface methodology for extraction optimization of germinant pumpkin seeds protein. Food Chem. 92, 701–706 (2005)
Wani, A.A., Sogi, D.S., Grover, L., Saxena, D.C.: Effect of temperature, alkali concentration, mixing time and meal/solvent ratio on the extraction of watermelon seed proteins-A response surface approach. Biosyst Eng. 94, 67–73 (2006)
Al-Farsi, M.A., Lee, C.Y.: Optimization of phenolics and dietary fibre extraction from date seeds. Food Chem. 108, 977–985 (2008)
Garau, M.C., Simal, S., Rossello, C., Femenia, A.: Effect of air-drying temperature on physico-chemical properties of dietary fibre and antioxidant capacity of orange (Citrus aurantium v. canoneta) by-products. Food Chem. 104, 1014–1024 (2007)
Bernadac, A., Latché, A., Roustan, J.P., Bouzayen, M., Pech, J.C.: Cucurbits, pepper, eggplant, legumes and other vegetables. In: Valpuesta, V. (ed.) Fruit and Vegetable Biotechnology, pp. 250–293. Woodhead Publishing, Cambridge (2002)
Li, M., Wen, X., Peng, Y., Wang, Y., Wang, K., Ni, Y.: Functional properties of protein isolates from bell pepper (Capsicum annuum L. var. Annuum) seeds. LWT-Food Sci. Technol. 97, 802–810 (2018)
Firatligil-Durmus, E., Evranuz, O.: Response surface methodology for protein extraction optimization of red pepper seed (Capsicum frutescens). LWT-Food Sci. Technol. 43, 226–231 (2010)
Minguez-Mosquera, M.I., Hornero-Mendez, D.: Separation and quantification of the carotenoid pigments in red peppers (Capsicum annuum L.), paprika, and oleoresin by reversed-phase HPLC. J. Agric. Food Chem. 41, 1616–1620 (1993)
Nath, P., Kale, S.J., Kaur, C., Chauhan, O.P.: Phytonutrient composition, antioxidant activity and acceptability of muffins incorporated with red capsicum pomace powder. J. Food Sci. Technol. 55, 2208–2219 (2018)
Kalak, T., Dudczak-Hałabuda, J., Tachibana, Y., Cierpiszewski, R.: Effective bioremoval of Fe (III) ions using paprika (Capsicum annuum L.) pomace generated in the food industry. J. Mater. Cycles Waste Manag. 23, 248–258 (2021)
Russ, W., Meyer-Pittroff, R.: Utilizing waste products from the food production and processing industries. Crit. Rev. Food Sci. Nutr. 44, 57–62 (2004)
FAO. Food and Agriculture Organization of the United Nations. FAO Statistical Databases: (2008). http://faostat.fao.org. Accessed December 2023
Roberta, M.S.A., Mariana, S.L.F., Édira, C.B.A.G.: Functional capacity of flour obtained from residues of fruit and vegetables. Int. Food Res. J. 21, 1675 (2014)
Lee, J.H., Yang, H.J., Lee, K.Y., Song, K.B.: Physical properties and application of a red pepper seed meal protein composite film containing oregano oil. Food Hydrocoll. 55, 136–143 (2016)
Chinma, C.E., Ariahu, C.C., Abu, J.O.: Development and characterization of cassava starch and soy protein concentrate based edible films. Int. J. Food Sci. Technol. 47, 383–389 (2012)
Cvetković, T., Ranilović, J., Gajari, D., Tomić-Obrdalj, H., Šubarić, D., Moslavac, T., Cikoš, A.-M., Jokić, S.: Podravka and slavonka varieties of pepper seeds (Capsicum annuum L.) as a new source of highly nutritional edible oil. Foods. 9, 1262 (2020)
Paraman, I., Hettiarachchy, N.S., Schaefer, C., Beck, M.I.: Physicochemical properties of rice endosperm proteins extracted by chemical and enzymatic methods. Cereal Chem. 83, 663–667 (2006)
Liu, C., Yu, B., Tao, H., Liu, P., Zhao, H., Tan, C., Cui, B.: Effects of soy protein isolate on mechanical and hydrophobic properties of oxidized corn starch film. LWT-Food Sci. Technol. 147, 111529 (2021)
Rangel-Marrón, M., Montalvo-Paquini, C., Palou, E., López-Malo, A.: Optimization of the moisture content, thickness, water solubility and water vapor permeability of sodium alginate edible films. Recent. Adv. Chem. Eng. Biochem. Computational Chem. 72–78 (2013)
Cao, N., Fu, Y., He, J.: Preparation and physical properties of soy protein isolate and gelatin composite films. Food Hydrocoll. 21, 1153–1162 (2007)
ASTM: Standard test Method for Water Vapor Transmission of materials – E96/E96M-15, West Conshohocken. PA: ASTM, Annual Book of American Standard Testing Methods (2015)
McHugh, T.H., Avena-Bustillos, R., Krochta, J.M.: Hydrophilic edible films: Modified procedure for water vapor permeability and explanation of thickness effects. J. Food Sci. 58, 899–903 (1993)
Yildirim-Yalcin, M., Seker, M., Sadikoglu, H.: Development and characterization of edible films based on modified corn starch and grape juice. Food Chem. 292, 6–13 (2019)
Khanzadi, M., Jafari, S.M., Mirzaei, H., Chegini, F.K., Maghsoudlou, Y., Dehnad, D.: Physical and mechanical properties in biodegradable films of whey protein concentrate–pullulan by application of beeswax. Carbohydr. Polym. 118, 24–29 (2015)
ASTM: Standard test Method for Transparency of Plastic sheeting – D1746-15, West Conshohocken. PA: ASTM, Annual Book of American Standard Testing Methods (2015)
ASTM: Standard test Method for Tensile Properties of thin Plastic sheeting – D882-12, West Conshohocken. ASTM, Annual Book of American Standard Testing Methods, PA (2012)
Cai, L., Cao, A., Aisikaer, G., Ying, T.: Influence of kernel roasting on bioactive components and oxidative stability of pine nut oil. Eur. J. Lipid Sci. Technol. 115, 556–563 (2013)
García, M.A., Pinotti, A., Martino, M.N., Zaritzky, N.E.: Characterization of starch and composite edible films and coatings. In: Embuscado, M.E., Huber, K.C. (eds.) Edible Films and Coatings for Food Applications, pp. 169–209. Springer, New York (2009)
Galus, S., Lenart, A.: Development and characterization of composite edible films based on sodium alginate and pectin. J. Food Eng. 115, 459–465 (2013)
McHugh, T.H., Aujard, J.F., Krochta, J.M.: Plasticized whey protein edible films: Water vapor permeability properties. J. Food Sci. 59, 416–419 (1994)
Bazzaz, A.E., Hakimzadeh, V., Noghabi, M.S.: Preparation and study of carboxymethyl cellulose biodegradable films properties containing Mentha pulegium essential oil. J. Thermoplast Compos. Mater. 34, 1213–1233 (2021)
Zhou, Y., Wu, X., Chen, J., He, J.: Effects of Cinnamon essential oil on the physical, mechanical, structural and thermal properties of cassava starch-based edible films. Int. J. Biol. Macromol. 184, 574–583 (2021)
Masamba, K., Li, Y., Hategekimana, J., Zehadi, M., Ma, J., Zhong, F.: Evaluation of mechanical and water barrier properties of transglutaminase cross-linked zein films incorporated with oleic acid. Int. J. Food Sci. 51, 1159–1167 (2016)
Pitak, N., Rakshit, S.K.: Physical and antimicrobial properties of banana flour/chitosan biodegradable and self sealing films used for preserving fresh-cut vegetables. LWT-Food Sci. Technol. 44, 2310–2315 (2011)
Pérez-Gago, M.B., Nadaud, P., Krochta, J.M.: Water vapor permeability, solubility, and tensile properties of heat‐denatured versus native whey protein films. J. Food Sci. 64, 1034–1037 (1999)
Gontard, N., Guilbert, S., Cuq, J.L.: Water and glycerol as plasticizers affect mechanical and water vapor barrier properties of an edible wheat gluten film. J. Food Sci. 58, 206–211 (1993)
Marzlan, A.A., Muhialdin, B.J., Abedin, N.H.Z., Manshoor, N., Ranjith, F.H., Anzian, A., Hussin, A.S.M.: Incorporating torch ginger (Etlingera elatior Jack) inflorescence essential oil onto starch-based edible film towards sustainable active packaging for chicken meat. Ind. Crops Prod. 184, 115058 (2022)
Lee, K.Y., Shim, J., Lee, H.G.: Mechanical properties of gellan and gelatin composite films. Carbohydr. Polym. 56, 251–254 (2004)
Lacroix, M.: Mechanical and permeability properties of edible films and coatings for food and pharmaceutical applications. In: Embuscado, M.E., Huber, K.C. (eds.) Edible Films and Coatings for Food Applications, pp. 347–366. Springer, New York (2009)
Ghasemlou, M., Khodaiyan, F., Oromiehie, A., Yarmand, M.S.: Development and characterisation of a new biodegradable edible film made from kefiran, an exopolysaccharide obtained from kefir grains. Food Chem. 127, 1496–1502 (2011)
Fang, Y., Tung, M.A., Britt, I.J., Yada, S., Dalgleish, D.G.: Tensile and barrier properties of edible films made from whey proteins. J. Food Sci. 67, 188–193 (2002)
Basiak, E., Galus, S., Lenart, A.: Characterisation of composite edible films based on wheat starch and whey-protein isolate. Int. J. Food Sci. 50, 372–380 (2015)
Carpiné, D., Dagostin, J.L.A., Bertan, L.C., Mafra, M.R.: Development and characterization of soy protein isolate emulsion-based edible films with added coconut oil for olive oil packaging: Barrier, mechanical, and thermal properties. Food Bioproc. Tech. 8, 1811–1823 (2015)
Valenzuela, C., Abugoch, L., Tapia, C.: Quinoa protein–chitosan–sunflower oil edible film: Mechanical, barrier and structural properties. LWT-Food Sci. Technol. 50, 531–537 (2013)
Liu, L., Kerry, J.F., Kerry, J.P.: Effect of food ingredients and selected lipids on the physical properties of extruded edible films/casings. Int. J. Food Sci. 41, 295–302 (2006)
Matthaus, B., Ozcan, M.M.: Chemical evaluation of some paprika (Capsicum annuum L.) seed oils. Eur. J. Lipid Sci. Technol. 111, 1249–1254 (2009)
Yılmaz, E., Arsunar, E.S., Aydeniz, B., Güneşer, O.: Cold pressed capia pepper (Capsicum annuum L.) oils: Composition, aroma and sensory properties. Eur. J. Lipid Sci. Technol. 117, 1016–1026 (2015)
Chouaibi, M., Rezig, L., Hamdi, S., Ferrari, G.: Chemical properties and compositions of red pepper seed oils extracted by different methods. Ind. Crops Prod. 128, 363–370 (2019)
Salih, A.A.: Extraction and identification of oil extract from Capsicum annuum L. fruits and study of its antimicrobial activity. Proteins, 12, (2006)
Shivaraju, V.K., Vallayil Appukuttan, S.: The influence of bound water on the FTIR characteristics of starch and starch nanocrystals obtained from selected natural sources. Starch-Stärke. 71, 1700026 (2019)
Choi, I., Shin, D., Lyu, J.S., Lee, J.S., Song, H.G., Chung, M.N., Han, J.: Physicochemical properties and solubility of sweet potato starch-based edible films. Food Packag. Shelf Life. 33, 100867 (2022)
Pozo, C., Rodríguez-Llamazares, S., Bouza, R., Barral, L., Castaño, J., Müller, N., Restrepo, I.: Study of the structural order of native starch granules using combined FTIR and XRD analysis. J. Polym. Res. 25, 1–8 (2018)
Gallagher, W.: FTIR analysis of protein structure. Course Man. Chem 455 (2009)
Chittur, K.K.: FTIR/ATR for protein adsorption to biomaterial surfaces. Biomaterials. 19, 357–369 (1998)
Bujok, J., Gąsior-Głogowska, M., Marszałek, M., Trochanowska-Pauk, N., Zigo, F., Pavľak, A., Komorowska, M., Walski, T.: Applicability of FTIR-ATR method to measure carbonyls in blood plasma after physical and mental stress. BioMed. Res. Int. (2019)
Acknowledgements
This work was funded by the Scientific and Technological Research Council of Turkey (TUBITAK, 2209B), Project No:1139B412101942.
Author information
Authors and Affiliations
Contributions
All authors contributed to the study conception and design. Material preparation, formal analysis, conceptualization, investigation and methodology were performed by Zeynep Kader Akıncı, Halil Karaman, Meral Yıldırım-Yalçın, Hatice Sena Olcay Mehmet Inan and Omer Said Toker. The first draft of the manuscript was written by Zeynep Kader Akıncı, Halil Karaman, Meral Yıldırım-Yalçın, Hatice Sena Olcay and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Ethical Approval
Ethical approval was not applicable for this research.
Conflict of Interest
The authors declare no conflict of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Akinci, Z.K., Karaman, H., Yildirim-Yalcin, M. et al. Effects of Red Pepper Pomace Protein and Oil on the Properties of Starch-Based Edible Films. Waste Biomass Valor 15, 3579–3588 (2024). https://doi.org/10.1007/s12649-023-02414-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12649-023-02414-y