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A Novel Method to Extract Juglone from Juglans mandshurica Waste Branches Using a Water-in-Oil Microemulsion

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Abstract

Pruning of Juglans mandshurica produces many branches, which are potentially a rich source of juglone although they are usually discarded as waste. Herein, we describe a water-in-oil microemulsion-based microwave-assisted extraction (MBMAE) method to isolate the juglone found in waste branches from J. mandshurica. In our experiment, J. mandshurica waste branch powder was added to the MBMAE system. Using the optimized microemulsion system comprising Tween 80:n-propanol:n-hexane:water at 27:13.5:4.5:55 (w/w/w/w), and a pH of 5.6, at an operating temperature of 40 °C, operating time of 63 s, and operating power of 400 W, the juglone yield was 4.58 mg/g from a ratio of microemulsion to branch power of 20:1 (mL/g). The juglone extraction yield using the MBMAE method was 1.86- and 6.65-fold better than that obtained from microwave-assisted extraction by ethanol (Ethanol-MAE) and heat reflux extraction by ethanol (Ethanol-HRE), respectively. The MBMAE method may be useful as an alternative to traditional extraction methods to isolate juglone.

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Acknowledgements

We thank Renee Mosi, PhD, from Liwen Bianji, Edanz Editing China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.

Funding

This work was financially supported by the Fundamental Research Fund for Central Universities (2572019CZ01), the 111 Project of China (B20088) and the Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team).

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Authors and Affiliations

  1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, China

    Chunying Li, Xianming Meng, Mengfei Tian, Shen Li, Yao Tian, Tingting Wang & Chunjian Zhao

  2. College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, China

    Chunying Li, Xianming Meng, Mengfei Tian, Shen Li, Yao Tian, Tingting Wang & Chunjian Zhao

  3. Collaborative Innovation Center for Development and Utilization of Forest Resources, Harbin, 150040, China

    Chunjian Zhao

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  1. Chunying Li
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  4. Shen Li
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Contributions

CZ and CL conceived the underlying ideas of the study, XM and MT contributed to the practical aspects of the research work, SL, YT and TW analyzed the data, and CL, XM and MT wrote the manuscript.

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Correspondence to Chunjian Zhao.

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We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work. There is no professional or other personal interest of any nature or kind in any product, service or company that could be construed as influencing the position presented in the manuscript entitled “A novel method to extract juglone from J. mandshurica waste branches using a water-in-oil microemulsion”.

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Li, C., Meng, X., Tian, M. et al. A Novel Method to Extract Juglone from Juglans mandshurica Waste Branches Using a Water-in-Oil Microemulsion. Waste Biomass Valor 13, 1547–1563 (2022). https://doi.org/10.1007/s12649-021-01611-x

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