Abstract
Effect of Isometamidium treatment of rats infected with Trypanosoma evansi was studied under experimental condition. The infection resulted in 100 per cent mortality by 7 day post treatment (DPT) in the infected untreated rats (Gr.II) and Isometamidium-HCl exerted 90 percent anti-trypanosomal efficacy. Haematological studies of all the groups on 3 day post infection (DPI) and on 7 DPT showed that T. evansi infection significantly decreased haematological values, however Isometamidium treatment significantly (P < 0.05) improved Hb and PCV without any significant changes in TEC and TLC values. Serum AST and ALT activities were significantly (P < 0.01) higher in the treated rats compared to healthy rats. Treatment with Isometamidium-HCl significantly improved blood glucose level but decreased creatinine level compared to pretreatment values. There was no significant change in BUN level of treated rats but the value was higher (P < 0.01) compared to healthy rats.
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Adedapo AA, Olayemi FO, Saba AB, Dina OA, Momoh FO (2005) An assessment of a prophylactic dose of Isometamidium Chloride on haematological and serum biochemical parameters of Rabbits experimentally infected with Trypanosoma brucei brucei. Folia-Veterinaria 49(3):138–142
Bhatia BB, Shah HL (2001) Protozoa and protozoan diseases of domestic livestock. Directorate of Information and Publication of Agriculture, Indian Council of Agricultural Research, New Delhi. pp. 202
Bhatia BB (2000) Text Book of Veterinary Protozoology. 1st Edn. Directorate of Information and Publication of Agriculture. Indian Council of Agricultural Research, Krishi Anusandhan Bhavan, Pusa, New Delhi. pp. 43
Boreham Pfl(1974) Physiopathological changes in the blood of rabbit infected with T. brucei. Rev. Elev. Med. vet. Pays trop. (Suppl.), 27:279–282
Brun R, Jenni L, Schonenberger M, Schell KF (1998) In vitro cultivation of blood stream form of Trypanosoma brucei, T. rhodesiense and T. gambiense. Journal of Protozoology, 28(4): 470–479
Chaka H, Abebe G (2003) Drug resistant trypanosomes: a threat to cattle production in the Southwest of Ethiopia. Revue-d′-Elevage-et-de-Medecine-Veterinaire-des-Pays-Tropicaux. 56(1/2):33–36
Chidi MHO, Nnamdi OK (2001) Therapeutic and prophylactic effects of Antrycide Prosalt(R) in Murine Trypanosomosis. Journal of Parasitic Diseases, 25(1):34–39
El Gendi, AYI, El Sayed MGA (1984) Effect of suramin and Isometamidium chloride on some serum enzymes and blood Constituents in camels. Bulletin of Animal Health and Production In Africa, 32(1):75–79
Folin O, Wu H (1919) Blood analysis; Determination of creatinine. In: Hawk’s Physiological chemistry. Bernard, LO (Ed.) 14th Edn. McGraw Hill Book Co., NewYork. pp.1040–1043
Gill BS (1977) Trypanosomes and Trypanosomiases of Indian Livestock. 1st Print. Indian Council of Agricultural Research, New Delhi
Jain NC (1993) Essentials of Veterinary Haematology. Lea and Febiger, Philadelphia.
Jones LM, Booth NH, McDonald LE (1982) Jones Veterinary Pharmacology and Therapeutics, Fifth Edition, Iowa State University Press
Kaneko JJ, Harvey JW, Bruss ML (1997) Clinical Biochemistry of Domestic Animals. Fifth Edition. Singapore: Academic Press.
Levine Norman D (1985) Veterinary Protozoology, 1st Edn., The Iowa State University, Ames, Iowa
Patel BK, Herangal DD, (1985) Trypanosomiasis in Gujarat state. In: Proceedings of seminar on Haemoprotozoan diseases of domestic Animals. Sponsored by the Commonwealth Veterinary Association, Organized by Department of Veterinary Medicine, Agricultural University, Hissar, India. pp 181–186
Prasad KD, Laha R (1990) Chemotherapeutic efficacy of Tribexin prosalt against experimental Trypanosoma evansi infection in mice. Journal of Research Birsa Agriculture University, 1(2):209–212
Samaddar J (1969) Surra in rabbits. Indian Veterinary Journal, 46:386
Samaddar J, Gill BS (1962) T. evansi infection in goats. Indian Journal of Microbiology, 2:63
Schalm OW, Jain NC, Carroll EJ (1986) Veterinary Haematology, 4th ed. Lea and Febiger, Philadelphia
Sen HG, Mukherjee AM, Ray HN (1959) Pathology of Trypanosoma evansi infection in rats. Indian Journal of Veterinary Sciences, 29:108–112
Silva RAMS, Ramirez L, Souza SS, Ortiz AG, Pereira SR, Davila AMR (1993) Hematology of natural bovine trypanosomosis in the Brazilian Pantanal and Bolivian wetlands. Veterinary Parasitology, 85(1):87–93
Singla LD, Juyal PD, Ahuja SP (2004) Serum protein changes of cross bred calves experimentally infected with Trypanosoma evansi. Indian Veterinary Journal, 77(2):172–174
Skeggs (1957) Blood analysis: Diacetyl monoxime method for estimating Urea nitrogen. In: Hawk’s Physiological Chemistry. Bernard, L.O. (Ed) 14th edn. Mc.Graw Hill Book Co., New York. pp 1038–1039
Soodan JS, Singh KB, Juyal PD, Khahra SS (1995) Incidence of Trypanosoma evansi infection in equines in Punjab state. Journal of Veterinary Parasitology, 9(2):133–134
Soulsby EJL (1982) Helminths, Arthropods and Protozoa of Domesticated animals. 7th Edn. The English Language Book Society, Bailliere Tindall, London
Tietz NW (1976) Clinical Guide to Laboratory Tests. (1st Edn.). Baillier, Tindall and Cassel Ltd., London, WC pp. 596
Toro, G, Ackerman PG (1975) Practical Clinical Chemistry, 1st edition, Little, Brown and Company, pp. 484
Toro M, Leon E, Lopez R, Pallota F, Garcia JA, Ruiz A (1983) Effect of isometamidium on infections by Trypanosoma vivax and T. evansi in experimentally-infected animals. Veterinary Parasitology, 13(1):35–43
Tuntasuvan D, Jarabrum W, Viseshakul N, Mohvaew K, Borisutsuwan S, Theeraphan A, Kongkanjana N (2003) Chemotherapy of surra in horses and mules with dimenazene aceturate. Veterinary Parasitology, 110(3–4):227–233
Wag WR, Washbourn K, Brown KN, Hill J (1958) Structure of isometamidium. Nature, 182:10
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Kumar, U., Jas, R. & Ghosh, J.D. Effect of isometamidium hydrochloride on Trypanosoma evansi infections in rats. J Parasit Dis 33, 36–41 (2009). https://doi.org/10.1007/s12639-009-0006-3
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DOI: https://doi.org/10.1007/s12639-009-0006-3