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Detection Bioactive Metabolites of Fructobacillus fructosus Strain HI-1 Isolated from Honey Bee’s Digestive Tract Against Paenibacillus larvae

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Abstract

American foulbrood is a devastating disease of honey bee, causing economic loss in the beekeeping industry. The disease mainly causes reduction in honey bee populations which negatively affect the honey bee’s major role as natural pollinators of significant crops and wildflowers. Thus, it is crucial to develop safe efficient strategies to control the disease and to improve bee colony health. Using lactic acid bacteria (LAB) as an alternative to chemical treatments is a promising novel technique for tackling honey bee diseases and improving their immunity. The endogenous LAB isolates were recovered from honey bee gut samples collected from different apiaries in two Egyptian governorates and screened for antagonistic activities against Paenibacillus larvae (pathogen of AFB disease). The results showed that 53.3% of tested LAB isolates (n = 120) exhibited antagonistic activities against P. larvae. The minimum inhibitory concentration and minimum bactericidal concentration of the most potent LAB isolate (with an inhibition zone of 44 mm) were 100 and 125 µL/mL, respectively. 16S rRNA sequencing identified the most potent isolate as Fructobacillus fructosus HI-1. The bioactive metabolites of F. fructosus were extracted with ethyl acetate and fractionated on thin-layer chromatography (TLC); also, bioactive fractions were detected. Heptyl 2-methylbutyrate, di-isobutyl phthalate, d-turanose, heptakis (trimethylsilyl), di-isooctyl phthalate, and hyodeoxycholic acid compounds were identified in the bioactive fractions. The result explores the promising administration of probiotic metabolites to control honey bee AFB disease, as a natural tool to substitute antibiotics and chemicals in disease-controlling strategies.

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Acknowledgements

The authors thank Dr. Samah N. Essa senior researcher, Pest Physiology Research Department, Plant Protection Research Institute, Agricultural Research Center, for her valuable help in identifying bioactive compounds. We would also like to thank Dr. Medhat Radi researcher, Pest Physiology Research Department, Plant Protection Research Institute, Agricultural Research Center, for construction the phylogenetic tree.

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Authors and Affiliations

  1. Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, Egypt

    Azza A. Abou Zeid & Ibrahim A. Abou El-Khair

  2. Honey Bee Research Department, Plant Protection Research Institute, Agricultural Research Center, Giza, Egypt

    Ahmed M. Khattaby & Hend I. A. Gouda

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  1. Azza A. Abou Zeid
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  2. Ahmed M. Khattaby
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  3. Ibrahim A. Abou El-Khair
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  4. Hend I. A. Gouda
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Idea: Ibrahim A. Abou El-Khair. Conceptualization and supervision: Azza A. Abou Zeid; Ahmed M. Khattaby. Investigation: Hend I.A. Gouda. Methodology: Hend I.A. Gouda. Manuscript draft writing: Hend I.A. Gouda. All authors read and approved the final manuscript.

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Correspondence to Hend I. A. Gouda.

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Zeid, A.A.A., Khattaby, A.M., El-Khair, I.A.A. et al. Detection Bioactive Metabolites of Fructobacillus fructosus Strain HI-1 Isolated from Honey Bee’s Digestive Tract Against Paenibacillus larvae. Probiotics & Antimicro. Prot. 14, 476–485 (2022). https://doi.org/10.1007/s12602-021-09812-5

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