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Lactobacillus plantarum BSGP201683 Improves the Intestinal Barrier of Giant Panda Microbiota-Associated Mouse Infected by Enterotoxigenic Escherichia coli K88

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Abstract

Giant pandas often suffered from gastrointestinal disease, especially the captive sub-adult one. Our study aims to investigate whether L. plantarum G83, a good panda-derived probiotic, can improve the intestinal barrier against the enterotoxigenic Escherichia coli K88 (E. coli K88) infection in giant panda microbiota-associated mice (GPAM). We treated SPF mice with antibiotics cocktail and transplanted the giant panda intestinal microbiota to set up a GPAM. Our results demonstrated that the microbiota of GPAM changed over time and was relatively stable in the short-term experiment (2–4 weeks). Whereafter, the GPAM pretreated with L. plantarum G83 for 15 days and infected with enterotoxigenic E. coli K88. The result indicated that the number of Bifidobacteria spp. increased in GPAM-G and GPAM-GE groups; the Lactobacillus spp. only increased in the GPAM-G group. Although the abundance of Enterobacteriaceae spp. only decreased in the GPAM-G group, the copy number of Escherichia coli in the GPAM-E group was significantly lower than that in the other groups. Meanwhile, the L. plantarum G83-induced alteration of microbiota could increase the mRNA expression of Claudin-1, Zo-1, and Occludin-1 in the GPAM-G group in the ileum; only Occludin-1 was increased in the GPAM-GE group. The sIgA in the ileum showed a positive response, also the result of body weight and histology in both the GPAM-G and GPAM-GE group. These results indicated that the L. plantarum G83 could improve the intestinal barrier to defense the enterotoxigenic E. coli K88 invasion.

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Funding

The present study supported by the National Natural Science Foundation of China (31970503), the Chengdu Giant Panda Breeding Research Foundation (CPF2014-15), and the China Scholarship Council (201906910016).

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Authors and Affiliations

  1. Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China

    Yi Zhou, Xueqin Ni, Qian Liu, Yan Zeng, Jie Wang, Abdul Khalique, Kangcheng Pan, Bo Jing & Dong Zeng

  2. Qu Country Extension Station for Animal Husbandry Technology, Dazhou, 635299, Sichuan, China

    Ling Duan

  3. Chengdu Wildlife Institute, Chengdu Zoo, Chengdu, 610081, Sichuan, China

    Lili Niu & Qiang Wang

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  1. Yi Zhou
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Correspondence to Dong Zeng.

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Zhou, Y., Ni, X., Duan, L. et al. Lactobacillus plantarum BSGP201683 Improves the Intestinal Barrier of Giant Panda Microbiota-Associated Mouse Infected by Enterotoxigenic Escherichia coli K88. Probiotics & Antimicro. Prot. 13, 664–676 (2021). https://doi.org/10.1007/s12602-020-09722-y

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