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Impact of Potential Probiotic Lactobacillus Strains on Host Growth and Development in a Drosophila melanogaster Model

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Abstract

Lactobacillus paracasei SD1, Lactobacillus rhamnosus SD11, and Lactobacillus gasseri SD12 were proposed as potential probiotics for oral health. However, the effects of them on host physiology are still unknown. This study aimed to select strains that can promote host growth and development in monocolonized Drosophila model compared with axenic and the commercial Lactobacillus rhamnosus GG–treated flies. The morphogenesis and growth of axenic Drosophila melanogaster were assessed from embryo to adult stage when provided with each probiotic strain–supplemented food. The colonization and persistence of probiotic in fly gut were also evaluated. The results indicated that axenic condition caused the lowest adult weight and emergence rate. All probiotic groups had higher weight than axenic group. Lact. rhamnosus SD11 group presented high adult emergence rate equivalent to Lact. rhamnosus GG group, which is significantly higher than the others. However, Lact. gasseri SD12 group had significantly lower adult weight and emergence rate when compared with Lact. rhamnosus GG group. The gut probiotics levels were rapidly increased within the first day after receiving probiotics. After probiotic cessation, their number in gut decreased and was maintained at low level, except for Lact. gasseri SD12, which completely vanished since day one. In conclusion, Lact. paracasei SD1, Lact. rhamnosus SD11, and Lact. rhamnosus GG can affect morphogenesis and weight of flies when fed since immature stage and have short period gut colonization. The findings of this study could possibly imply comparable health-promoting effects between Lact. paracasei SD1 and Lact. rhamnosus SD11 to commercial strain in Drosophila model.

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Acknowledgements

We thank all staff of the Oral Microbiology Laboratory, Faculty of Dentistry, and the Basic Science 1008 Laboratory, Faculty of Science, Prince of Songkla University, for helpful suggestions and comments.

Funding

This study was supported by the Graduate School and Faculty of Dentistry, Prince of Songkla University.

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Authors and Affiliations

  1. Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, Thailand

    Pasaraporn Laomongkholchaisri & Supatcharin Piwat

  2. Common Oral Diseases and Epidemiology Research Center, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, Thailand

    Pasaraporn Laomongkholchaisri, Rawee Teanpaisan & Supatcharin Piwat

  3. Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, Thailand

    Rawee Teanpaisan

  4. Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat-Yai, Thailand

    Monwadee Wonglapsuwan

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  1. Pasaraporn Laomongkholchaisri
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Correspondence to Supatcharin Piwat.

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This study was approved by the Institutional Animal Care and Use Committee, Prince of Songkla University (MOE 0521.11/380).

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Laomongkholchaisri, P., Teanpaisan, R., Wonglapsuwan, M. et al. Impact of Potential Probiotic Lactobacillus Strains on Host Growth and Development in a Drosophila melanogaster Model. Probiotics & Antimicro. Prot. 13, 390–397 (2021). https://doi.org/10.1007/s12602-020-09705-z

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