Abstract
Foliar samples were collected from the tomato (Solanum lycopersicum) cultivar ‘IPA-6’ exhibiting begomovirus-like symptoms (apical leaf curling and chlorosis) in Iranduba (Brazilian Amazon). PCR tests with degenerate primers targeting DNA–A and DNA–B components of a sample designated as AM–035 were positive for both genomic regions. Full-length cognate DNA–A and DNA–B components of AM–035 were cloned and sequenced via Sanger dideoxy termination reaction. The complete sequence of the DNA–A component (MK878452) comprised 2,630 nucleotides (organized in five open reading frames – ORFs), and the DNA–B component (MK878451) displayed 2,593 nucleotides (two ORFs). The highest identities ranged from 90 to 92% with tomato chlorotic mottle Guyane virus (ToCMoGV) isolates from French Guiana, indicating a highly divergent Brazilian strain of this virus. The present work reinforces the scenario of the high diversity of tomato-infecting begomoviruses in Brazil. Further studies are needed to determine the distribution and prevalence of ToCMoGV across tomato-producing areas in the Brazilian Amazon River Basin.
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Acknowledgements
The authors are grateful to the financial support of Capes (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), FAP-DF (Fundação de Apoio à Pesquisa do DF), and Embrapa (Empresa Brasileira de Pesquisa Agropecuária). The authors would like to thank Antonio Francisco Costa (CNPH—Embrapa Hortaliças) for their help and support in Sanger dideoxy termination reaction sequencing. We are deeply grateful to anonymous reviewers for their invaluable suggestions.
Funding
Financial support was provided by grants from Embrapa (Empresa Brasileira de Pesquisa Agropecuária), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), and FAP-DF (Fundação de Apoio à Pesquisa do Distrito Federal). This study was also financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Finance Code 001.
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Conceptualization, I.A.O., M.E.N.F.B., L.S.B. and R.C.P.C.; methodology, I.A.O., M.R.R., A.R., G.R.L., V.P.M.R.V., and R.C.P.C.; software, L.N.A.R., I.A.O. and R.C.P.C.; validation, R.C.P.C.; formal analysis, I.AO., R.C.P.C.; and L.N.A.R.; investigation, I.A.O., N.A.N.F., R.C.P.C., and V.P.M.R.G., resources, R.C.P.C., data curation, R.C.P.C., M.E.N.F.B., L.S.B., writing—original draft, I.A.O., L.S.B. and R.C.P.C.; preparation, I.A.O., L.R.G., L.S.B. and R.C.P.C.; writing—review and editing, M.R.R., R.L.G., I.A.O., L.S.B. and R.C.P.C.; visualization, L.S.B. and R.C.P.C.; supervision, L.S.B. and R.C.P.C.; project administration, R.L.G., L.S.B. and R.C.P.C.; funding acquisition, R.L.G., M.E.N.F.B., L.S.B. and R.C.P.C. All authors have read and agreed to the published version of the manuscript.
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de Oliveira, I.A., dos Reis, L.d.N.A., Nery, F.M.B. et al. Genomic characterization of a highly divergent tomato chlorotic mottle Guyane virus strain in the Brazilian Amazon River Basin. Phytoparasitica 52, 13 (2024). https://doi.org/10.1007/s12600-024-01122-0
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DOI: https://doi.org/10.1007/s12600-024-01122-0