Skip to main content
SpringerLink
Log in

Experimental transmission of the mild strain of Tomato yellow leaf curl virus (TYLCV) to Amaranthus dubius by Bemisia tabaci

  • Published:
Phytoparasitica Aims and scope Submit manuscript

Abstract

Tomato yellow leaf curl virus (TYLCV) is a monopartite begomovirus from the Old World. The mild strain of this virus (TYLCV-Mld) was described for South America in Venezuela in 2007. Due to the potential risks of establishment of this virus in the field, six common weeds were evaluated for susceptibility to an isolate of TYLCV-Mld by using adults of Bemisia tabaci (Hemiptera: Aleyrodidae) to mediate viral transmission. In this work, detection based on PCR amplification with TYLCV specific primers showed Amaranthus dubius (Amaranthaceae) as the only infected weed. In A. dubius, viral symptoms were observed from 11.0 ± 1.3 days post-inoculation and the transmission rate of TYLCV-Mld to this plant species was 83.3%. The successful back-transmission of TYLCV-Mld from A. dubius to tomato plants was demonstrated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Abou-Jawdah, Y., Maalouf, R., Shebaro, W., & Soubra, K. (1999). Comparison of the reaction of tomato lines to infection by tomato yellow leaf curl begomovirus in Lebanon. Plant Pathology, 48, 727–734.

    Article  Google Scholar 

  • Chirinos, D. T., Güerere, P., Geraud-Pouey, F., Romay, G., Santana, M. A., & Bastidas, L. (2009). Transmisión experimental de Tomato yellow leaf curl virus (TYLCV) por Bemisia tabaci (Aleyrodidae) a solanáceas. Revista Colombiana de Entomología, 35, 22–27.

    Google Scholar 

  • Cohen, S., Kern, J., Harpaz, I., & Ben-Joseph, R. (1988). Epidemiological studies of the Tomato yellow leaf curl virus (TYLCV) in the Jordan Valley, Israel. Phytoparasitica, 16, 259–270.

    Article  Google Scholar 

  • Czosnek, H., Ghanim, M., & Ghanim, M. (2002). The circulative pathway of begomoviruses in the whitefly vector Bemisia tabaci. Insights from studies with Tomato yellow leaf curl virus. Annals of Applied Biology, 140, 215–231.

    Article  Google Scholar 

  • Czosnek, H., & Laterrot, H. (1997). A worldwide survey of tomato yellow leaf curl viruses. Archives of Virology, 142, 1391–1406.

    Article  PubMed  CAS  Google Scholar 

  • De Barro, P. J., Liu, S., Boykin, L. M., & Dinsdale, A. B. (2011). Bemisia tabaci: a statement of species status. Annual Review of Entomology, 56, 1–19.

    Article  PubMed  Google Scholar 

  • Díaz-Pendón, J. A., Cañizares, M. C., Moriones, E., Bejarano, E. R., Czosnek, H., & Navas-Castillo, J. (2010). Tomato yellow leaf curl viruses: ménage à trois between the virus complex, the plant and the whitefly vector. Molecular Plant Pathology, 11, 441–450.

    Article  PubMed  Google Scholar 

  • Duffy, S., & Holmes, E. C. (2007). Multiple introductions of the Old World begomovirus Tomato yellow leaf curl virus into the New World. Applied and Environmental Microbiology, 73, 7114–7117.

    Article  PubMed  CAS  Google Scholar 

  • Ferrarotto, M. (2010). Respuesta de pira (Amaranthus dubius Mart. ex Tell.) y amaranto (Amaranthus cruentus L.) al déficit hídrico. Revista Facultad de Agronomía (UCV), 36, 20–27.

    Google Scholar 

  • Gámez-Jiménez, C., Romero-Romero, J. L., Santos-Cervantes, M. E., Leyva-López, N. E., & Méndez-Lozano, J. (2009). Tomatillo (Physalis ixocarpa) as a natural new host for Tomato yellow leaf curl virus in Sinaloa, México. Plant Disease, 93, 545.

    Article  Google Scholar 

  • Geraud, F., Chirinos, D., Romay, G., Santana, M., Bastidas, L., Fernández, C., et al. (2009). Transmisión del virus TYLCV a diferentes materiales de tomate (Solanum lycopersicum L.) mediada por el Biotipo B del complejo Bemisia tabaci (Gennadius). Bioagro, 21, 23–31.

    Google Scholar 

  • Gilbertson, R. L., Rojas, M. R., Russell, D. R., & Maxwell, D. P. (1991). Use of the asymmetric polymerase chain reaction and DNA sequencing to determine genetic variability of bean golden mosaic geminivirus in the Dominican Republic. Journal of General Virology, 72, 2843–2848.

    Article  PubMed  CAS  Google Scholar 

  • Hull, R. (2002). Matthews’ plant virology. San Diego, CA: Elsevier/Academic Press.

    Google Scholar 

  • Jordá, C., Font, I., Martínez, P., Juarez, M., Ortega, A., & Lacasa, A. (2001). Current status and new natural hosts of Tomato yellow leaf curl virus (TYLCV) in Spain. Plant Disease, 85, 445.

    Article  Google Scholar 

  • Lefeuvre, P., Hoareau, M., Delatte, H., Reynaud, B., & Lett, J. M. (2007). A multiplex PCR method discriminating between the TYLCV and TYLCV-Mld clades of Tomato yellow leaf curl virus. Journal of Virological Methods, 144, 165–168.

    Article  PubMed  CAS  Google Scholar 

  • Lefeuvre, P., Martin, D. P., Harkins, G., Lemey, P., Gray, A. J. A., Meredith, S., et al. (2010). The spread of Tomato yellow leaf curl virus from the Middle East to the world. PLoS Pathogens, 6(10), 1–12.

    Article  Google Scholar 

  • Ling, K., Simmons, A. M., Hassell, R. L., Keinath, A. P., & Polston, J. E. (2006). First report of Tomato yellow leaf curl virus in South Carolina. Plant Disease, 90, 379.

    Article  Google Scholar 

  • Morales, F. J., & Anderson, P. K. (2001). The emergence and dissemination of whitefly-transmitted geminiviruses in Latin America. Archives of Virology, 146, 415–441.

    Article  PubMed  CAS  Google Scholar 

  • Papayiannis, L. C., Katis, N. I., Idris, A. M., & Brown, J. K. (2011). Identification of weed hosts of Tomato yellow leaf curl virus in Cyprus. Plant Disease, 95, 120–125.

    Article  Google Scholar 

  • Polston, J. E., Bois, D., Serra, C. A., & Concepcion, S. (1994). First report of a tomato yellow leaf curl-like geminivirus in the Western Hemisphere. Plant Disease, 78, 831.

    Article  Google Scholar 

  • Romay, G., Geraud-Pouey, F., Chirinos, D. T., Santana, M. A., Galindo-Castro, I., & Márquez, L. M. (2011). Microsatellites reveal widespread predominance of an invasive over an indigenous Bemisia tabaci in Venezuela. Phytoparasitica, 39, 419–428.

    Article  Google Scholar 

  • Salas, J., & Arnal, E. (2001). Bemisia tabaci (Gennadius, 1899) biotipo B, primer registro para Venezuela utilizando RAPD’s- PCR. Entomotropica, 16, 181–185.

    Google Scholar 

  • Sánchez-Campos, S., Navas-Castillo, J., Monci, F., Díaz, J. A., & Moriones, E. (2000). Mercurialis ambigua and Solanum luteum: two newly discovered natural hosts of Tomato yellow leaf curl geminiviruses. European Journal of Plant Pathology, 106, 391–394.

    Article  Google Scholar 

  • Stanley, J., Bisaro, D. M., Briddon, R. W., Brown, J. K., Fauquet, C. M., Harrison, B. D., et al. (2005). Geminiviridae. In C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger, & L. A. Ball (Eds.), Virus taxonomy, VIII report of ICTV (pp. 301–326). London: Elsevier/Academic Press.

    Google Scholar 

  • Zambrano, K., Carballo, O., Geraud, F., Chirinos, D., Fernández, C., & Marys, E. (2007). First report of Tomato yellow leaf curl virus in Venezuela. Plant Disease, 91, 768.

    Article  Google Scholar 

Download references

Acknowledgments

We thank Juan Antonio Díaz-Pendón (Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, UMA-CSIC, Malaga, Spain) for providing the TYLCV southern blot hybridization test. This work was co-financed by the Venezuelan Ministry of Science, Technology and Industry (grant N° G-2000001610), Cuba-Venezuela Cooperation Agreement (grant N° 20070071) and Red Iberoamericana de Manejo Integrado de Enfermedades Virales en Hortalizas, Network 11RT0433, Fundación CYTED.

Author information

Authors and Affiliations

  1. Departamento de Ciencias Agropecuarias, Instituto Universitario de Tecnología de Maracaibo, Maracaibo, 4005, Estado Zulia, Venezuela

    P. Güerere

  2. La Universidad del Zulia (LUZ), Facultad de Agronomía, Unidad Técnica Fitosanitaria, La Universidad del Zulia, Maracaibo, 4005, Estado Zulia, Venezuela

    D. T. Chirinos & F. Geraud-Pouey

  3. Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” (IHSM-UMA-CSIC), Algarrobo-Costa, Málaga, Spain

    E. Moriones

  4. Departamento de Biología Celular, Carretera Nacional Hoyo de la Puerta-Baruta, Universidad Simón Bolívar (USB), Sartenejas, Caracas, 1080, Venezuela

    M. A. Santana

  5. Fundación Instituto de Estudios Avanzados (IDEA), Carretera Nacional Hoyo de la Puerta-Baruta, Sartenejas, Caracas, 1080, Venezuela

    M. A. Santana, M. A. Franco, I. Galindo-Castro & G. Romay

Authors
  1. P. Güerere
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. T. Chirinos
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Geraud-Pouey
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Moriones
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. A. Santana
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. A. Franco
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. I. Galindo-Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. G. Romay
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. Romay.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Güerere, P., Chirinos, D.T., Geraud-Pouey, F. et al. Experimental transmission of the mild strain of Tomato yellow leaf curl virus (TYLCV) to Amaranthus dubius by Bemisia tabaci . Phytoparasitica 40, 369–373 (2012). https://doi.org/10.1007/s12600-012-0237-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12600-012-0237-1

Keywords

Search

Navigation