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Photocatalytic degradation of environmental perilous gentian violet dye using leucaena-mediated zinc oxide nanoparticle and its anticancer activity

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Abstract

Phytomediated synthesis of metal oxide nanoparticles has become a key research area in nanotechnology due to its wide applicability in various biomedical fields. The present work explores the biosynthesis of zinc oxide nanoparticles (ZnO-NPs) using Leucaena leucocephala leaf extract. The synthesised ZnO-NPs were characterised by ultraviolet–visible (UV–Vis) spectroscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and selected area electron diffraction (SAED) studies. Biosynthesised ZnO-NPs are found to have wurtzite hexagonal structure with particles distributed in the range of 50–200 nm as confirmed by TEM studies. The anticancer activity of ZnO-NPs against MCF-7 (breast cancer) and PC-3 (human prostate cancer) cell lines was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. From the assay, biosynthesised ZnO-NPs have better cytotoxic activity on PC-3 cell lines than MCF-7 cell lines. The in vitro cytotoxicity studies of biosynthesised ZnO-NPs against Dalton lymphoma ascites (DLA) cells reveal better antitumor activity of 92% inhibition with concentration of 200 µg·ml−1 of ZnO-NPs, and as the concentration increases, the anticancer efficiency as well increases, and also, it has excellent photocatalytic activity to degrade crystal violet dye in aqueous solution after irradiation of 90 min. The result suggests that the green synthesis of ZnO-NPs could be easily recovered and reused several times without any significant loss of the catalytic activity. The advantage of this technique lies in its low cost, easily climbable and non-use of toxic agents.

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References

  1. Madhumita G, Elango G, Roopan SM. Bio-functionalised doped silver nanoparticles and its antimicrobial studies. J Solgel Sci Technol. 2015;73(2):476.

    Article  CAS  Google Scholar 

  2. Wang ZL. Zinc oxide nanostructures: growth, properties and applications. J Phys Condens Matter. 2004;16(25):R829.

    Article  CAS  Google Scholar 

  3. Zhang Y, Ram MK, Stefanakos EK, Goswami DY. Synthesis, characterization, and applications of ZnO nanowires. J Nanomater. 2012;2012(20):22.

    Google Scholar 

  4. Zhang Y, Fu L, Han F, Wang A, Cai W, Yang J, Peng F. Green biosynthesis and characterization of zinc oxide nanoparticles using Corymbia citriodora leaf extract and their photocatalytic activity. Green Chem Lett Rev. 2015;8(2):59.

    Article  CAS  Google Scholar 

  5. Shukla SK, Agorku ES, Mittal H, Mishra AK. Synthesis, characterization and photo luminescence properties of Ce3+-doped ZnO-nanophosphors. Chem Pap. 2014;68(2):217.

    Article  CAS  Google Scholar 

  6. Gomez-Solis C, Ballesteros JC, Torres-Martinez LM, Juarez-Ramirez I, Diaz Torres LA, Elvira Zarazua-Morin M, Lee SW. Rapid synthesis of ZnO nano-corncobs from Nital solution and its application in the photodegradation of methyl orange. J Photochem Photobiol A. 2015;298(1):49.

    Article  CAS  Google Scholar 

  7. Afifi M, Almaghrabi OA, Kadasa NM. Ameliorative effect of zinc oxide nanoparticles on antioxidants and sperm characteristics in streptozotocin-induced diabetic rat testes. Biomed Res Int. 2015. https://doi.org/10.1155/2015/153573.

    Article  Google Scholar 

  8. Chen J, Liu X, Wang C, Yin SS, Li XL, Hu WJ, Simon M, Shen ZJ, Ziao Q, Chu CC, Peng XX, Zheng HL. Nitric oxide ameliorates zinc oxide nanoparticles-induced phytotoxicity in rice seedlings. J Hazard Mater. 2015;297(30):173.

    Article  CAS  Google Scholar 

  9. Dhandapani P, Siddarth AS, Kamalasekaran S, Maruthamuthu S, Rajagopal G. Bio-approach: ureolytic bacteria mediated synthesis of ZnO nanocrystals on cotton fabric and evaluation of their antibacterial properties. Carbohydr Polym. 2014;103(15):448.

    Article  CAS  Google Scholar 

  10. Mitra S, Patra P, Pradhan S, Debnath N, Dey KK, Sarkar S, Chattopadhyay D, Goswami A. Microwave synthesis of ZnO@mSiO2 for detailed antifungal mode of action study: understanding the insights into oxidative stress. J Colloid Interface Sci. 2015;444(15):97.

    Article  CAS  Google Scholar 

  11. Agarwal H, Venkat Kumar S, Rajesh Kumar S. A review on green synthesis of zinc oxide nanoparticles: an eco-friendly approach. Resour effic Technol. 2017;3(4):406.

    Article  Google Scholar 

  12. Kanagamani K, Muthukrishnan P, Ilayaraja M, Vinothkumar J, Shankar K, Kathirasen A. Synthesis of Leucaena mediated silver nanoparticles assessing their photocatalytic degradation of Cr(VI) and in vitro cytotoxicity against DLA cells. J Photochem Photobiol A. 2017;346(1):470.

    Article  CAS  Google Scholar 

  13. Jamdagni P, Khatri P, Rana JS. Green synthesis of zinc oxide nanoparticles using flower extract of Nyctanthes arbor-tristis and their antifungal activity. J King Saud Univ Sci. 2018;30(2):168.

    Article  Google Scholar 

  14. Mishra V, Sharma R, Dut Jasuja N, Gupta DK. A review on green synthesis of nanoparticles and evaluation of antimicrobial activity. Int J Green Herb Chem. 2014;3(1):081.

    Google Scholar 

  15. Hussain I, Singh NB, Singh A, Singh H, Singh SC. Green synthesis of nanoparticles and its potential application. Biotechnol Lett. 2016;38(4):545.

    Article  CAS  Google Scholar 

  16. Dobrucka R, Dugaszewska J. Biosynthesis and antibacterial activity of ZnO nanoparticles using Trifolium pratense flower extract. Saudi J Biol Sci. 2016;23(4):517.

    Article  CAS  Google Scholar 

  17. Prashanth GK, Prashanth PA, Bora U, Gadewar M, Nagabhushana BM, Anand S, Krishnaiah GM, Sathyanand HM. In vitro antibacterial and cytotoxicity studies of ZnO nanopowders prepared by combustion assisted facile green synthesis. Karbala Int J Mod Sci. 2015;1(2):67.

    Article  Google Scholar 

  18. Singh KP, Gupta S, Singh AK, Sinha S. Optimizing adsorption of crystal violet dye from water by magnetic nanocomposite using response surface modeling approach. J Hazard Mater. 2011;186(2–3):1462.

    Article  CAS  Google Scholar 

  19. Ghosh D, Bhattacharyya KG. Adsorption of methylene blue on kaolinite. Appl Clay Sci. 2002;20(6):295.

    Article  CAS  Google Scholar 

  20. Ganesan S, Ganesh Babu I, Mahendran D, Indra Arulselvi P, Elangovan N, Geetha N, Venkatachalam P. Green engineering of titanium dioxide nanoparticles using Ageratina altissima (L.) King & H.E. Robines. medicinal plant aqueous leaf extracts for enhanced photocatalytic activity. Ann Phytomed. 2016;5(2):69.

    Article  CAS  Google Scholar 

  21. Senthilkumar S, Ashok M, Kashinath L, Sanjeeviraja C, Rajendran A. Phytosynthesis and characterization of TiO2 nanoparticles using Diospyros ebenum leaf extract and their antibacterial and photocatalytic degradation of crystal violet. Smart Sci. 2018;6(1):1.

    Article  Google Scholar 

  22. Begum T, Gogoi PK, Bora U. Photo degradation of crystal violet dye on the surface of Au doped TiO2 nanoparticles. Indian J Chem. 2017;24(1):97.

    CAS  Google Scholar 

  23. Tripathy N, Ahmad R, Kuk H, Lee DH, Hahn YB, Khang G. Rapid methyl orange degradation using porous ZnO spheres photocatalyst. J Photochem Photobiol B. 2016;161(1):312.

    Article  CAS  Google Scholar 

  24. Ramakrishnan R, Devaki SJ, Aashish A, Thomas S, Varma MR, Najiya KPP. Nanostructured semiconducting PEDOT-TiO2/ZnO hybrid composites for nanodevice applications. J Phys Chem C. 2016;120(8):4199.

    Article  CAS  Google Scholar 

  25. Manthina V, Agrios AG. Single-pot ZnO nanostructure synthesis by chemical bath deposition and their applications. Nano-Struct Nano-Object. 2016;7(1):1.

    Article  CAS  Google Scholar 

  26. Wilson AP. Cytotoxicity and Viability Assays in Animal Cell Culture: A Practical Approach. 3rd ed. Oxford: Oxford University Press; 2000. 175.

    Google Scholar 

  27. Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods. 1986;89(2):271.

    Article  CAS  Google Scholar 

  28. Bhuyana T, Mishraa K, Khanujab M, Prasada R, Varmaa A. Biosynthesis of zinc oxide nanoparticles from Azadirachta indica for antibacterial and photocatalytic applications. Mater Sci Semicond Process. 2015;32(1):55.

    Article  CAS  Google Scholar 

  29. Sangeethaa G, Rajeshwari S, Venckatesh R. Green synthesis of zinc oxide nanoparticles by aloebarbadensis miller leaf extract Structure and optical properties. Mater Res Bull. 2011;5(4):922.

    Google Scholar 

  30. Abdul Salam H, Sivaraj R, Venckatesh R. Green synthesis and characterization of zinc oxide nanoparticles from Ocimum basilicum L. var. purpurascens Benth.-Lamiaceae leaf extract. Mater Lett. 2014;131(15):16.

    Article  CAS  Google Scholar 

  31. Azizia S, Ahmada MB, Namvarb F, Mohamad R. Green biosynthesis and characterization of zinc oxide nanoparticles using brown marine macroalga Sargassum muticum aqueous extract. Mater Lett. 2014;116(1):275.

    Article  CAS  Google Scholar 

  32. Vanathi P, Rajiv P, Narendhran S, Rajeshwari S, Pattanathu Rahman KSM, Venckatesh R. Biosynthesis and characterization of phyto mediated zinc oxidenanoparticles: a green chemistry approach. Mater Lett. 2014;134(1):13.

    Article  CAS  Google Scholar 

  33. Chung IM, Abdul Rahuman A, Marimuthu S, Vishnu Kirthi A, Anbarasan K, Rajakumar G. An investigation of the cytotoxicity and Caspase-mediated apoptotic effect of green synthesized Zinc oxide nanoparticles using Eclipta prostrata on Human Liver Carcinoma Cells. Nanomater Basel. 2015;5(3):1317.

    Article  CAS  Google Scholar 

  34. Mirzaei H, Darroudi M. Zinc oxide nanoparticles: biological synthesis and biomedical applications. Ceram Int. 2017;43(1):907.

    Article  CAS  Google Scholar 

  35. Nagajyothia PC, Sreekanth TVM, Tetteya CO, Juna YI, Mooka SH. Characterization, antibacterial, antioxidant and cytotoxic activities of ZnO nanoparticles using Coptidis Rhizoma. Bioorg Med Chem Lett. 2014;24(17):4298.

    Article  CAS  Google Scholar 

  36. Vishnu Kiran M, Murugesan S. In vitro cytotoxic activity of silver nanoparticle biosynthesized from Colpomenia sinuosa and Halymenia poryphyroides using DLA and EAC cell lines. World J Pharm Sci. 2014;2(9):926.

    Google Scholar 

  37. Taccola L, Raffa V, Riggio C, Vittorio O, Iorio MC, Vanacore R, Pietrabissa A, Cuschieri A. Zinc oxide nanoparticles as selective killers of proliferating cells. Int J Nanomed. 2011;6:1129.

    CAS  Google Scholar 

  38. Boroumand Moghaddam A, Moniri M, Azizi S, Abdul Rahim R, Bin Ariff A, Navaderi M, Mohamad R. Eco-friendly formulated zinc oxide nanoparticles: induction of cell cycle arrest and apoptosis in the MCF-7 cancer cell line. Genes. 2017;8(10):281.

    Article  CAS  Google Scholar 

  39. Lv J, Gong W, Huang K, Zhu J, Meng F, Song X, Sun Z. Effect of annealing temperature on photocatalytic activity of ZnO thin films prepared by sol-gel method. Superlattice Microstruct. 2011;50(2):98.

    Article  CAS  Google Scholar 

  40. Khan MM, Lee J, Cho MH. Au@TiO2 nano composites for the catalytic degradation of methyl orange and methylene blue. An electron relay effect. J Ind Eng Chem. 2014;20(4):1584.

    Article  CAS  Google Scholar 

  41. Tripathy N, Ahmad R, Eun Song J, Park H, Khang G. ZnO nanonails for photocatalytic degradation of crystal violet dye under UV irradiation. Mater Sci. 2017;4(1):267.

    Google Scholar 

  42. Yang SJ, Xu YL, Gong WP, Huang YK, Wang GH, Yang Y, Fen CQ. Photocatalytic degradation of organic dyes with H3PW12O40/TiO2–SiO2. Rare Met. 2016;35(10):797.

    Article  CAS  Google Scholar 

  43. Yu CL, Wei LF, Chen JC, Zhou WQ, Fan QZ, Yu J. Novel AgCl/Ag2CO3 heterostructured photocatalysts with enhanced photocatalytic performance. Rare Met. 2016;35(6):475.

    Article  CAS  Google Scholar 

  44. Ren GX, Yu B, Liu YM, Wang HX, Zhang WG, Liang W. High photocatalytic activity of Cu2O/TiO2/Pt composite films prepared by magnetron sputtering. Rare Met. 2017;36(10):821.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, SNS College of Technology, Coimbatore, 641035, India

    Krishnaswamy Kanagamani

  2. Department of Chemistry, Faculty of Engineering, Karpagam Academy of Higher Education, Coimbatore, 641021, India

    Krishnaswamy Kanagamani, Pitchaipillai Muthukrishnan, Karikalan Shankar & Ayyasami Kathiresan

  3. Department of Chemistry, VHNSN College, Virudhunagar, 626001, India

    Karunamoorthy Saravanakumar

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  1. Krishnaswamy Kanagamani
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  2. Pitchaipillai Muthukrishnan
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  3. Karunamoorthy Saravanakumar
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  5. Ayyasami Kathiresan
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Correspondence to Pitchaipillai Muthukrishnan.

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Kanagamani, K., Muthukrishnan, P., Saravanakumar, K. et al. Photocatalytic degradation of environmental perilous gentian violet dye using leucaena-mediated zinc oxide nanoparticle and its anticancer activity. Rare Met. 38, 277–286 (2019). https://doi.org/10.1007/s12598-018-1189-5

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  • DOI: https://doi.org/10.1007/s12598-018-1189-5

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