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Cloning and mRNA expression of selenium-dependent glutathione peroxidase from the oyster Magallana hongkongensis in response to cadmium exposure and aerial exposure at different temperatures

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Abstract

Glutathione peroxidase is an important enzyme in the endogenous antioxidant defense system. In the current study, we cloned the full-length cDNA sequence that encodes a glutathione peroxidase from Magallana hongkongensis (designated as MhGPx), investigated the tissue expression profile of MhGPx mRNA, and studied the time-dependent effects of cadmium exposure and air exposure at two temperatures (4 °C and 15 °C) on the mRNA level of MhGPx in different tissues (including gill, adductor, mantle, and digestive gland). The full length of MhGPx was 1306 bp. This gene was found to contain a selenocysteine insertion sequence within the 3′ untranslated region, which indicates that the protein it encodes was a selenoprotein. In addition, MhGPx was constitutively expressed in all four selected tissues, and the level of expression in the digestive gland was highest, indicating that MhGPx mRNA expression has tissue-specificity. Under cadmium exposure, mRNA expression was significantly increased on day 1. When exposed to air, the highest MhGPx mRNA expression in the 4 °C group was lower and appeared later than that of the 15 °C group. The results of this study contribute to a better understanding of oyster GPx in response to oxidative stress.

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Acknowledgements

This work was supported by the National Key R&D Program of China (2018YFD0901105), the Natural Science Foundation of Guangdong Province (2017A030310626), the program for scientific research start-up funds of Guangdong Ocean University, and the special fund for the Construction of Modern Agricultural Industrial Technology System (No. CARS-49).

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Authors and Affiliations

  1. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088, China

    Citing Chen, Jialong Gao, Chaohua Zhang, Xiaoming Qin, Wenhong Cao, Huina Zheng & Haisheng Lin

  2. Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, National Research and Development Branch Center for Shellfish Processing (Zhanjiang), Zhanjiang, 524088, China

    Jialong Gao, Chaohua Zhang, Xiaoming Qin, Wenhong Cao, Huina Zheng & Haisheng Lin

  3. Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, 116034, China

    Jialong Gao, Chaohua Zhang, Xiaoming Qin, Wenhong Cao, Huina Zheng & Haisheng Lin

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  1. Citing Chen
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  2. Jialong Gao
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Contributions

Methodology JLG and CHZ. Validation JLG and WHC. Formal analysis JLG and CTC. Investigation JLG and CTC. Resources WHC, HNZ, and HSL. Data curation JLG and CHZ. Writing—original draft preparation JLG and CTC. Writing—review and editing JLG. Supervision CHZ. Project administration WHC and XMQ. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Jialong Gao.

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Chen, C., Gao, J., Zhang, C. et al. Cloning and mRNA expression of selenium-dependent glutathione peroxidase from the oyster Magallana hongkongensis in response to cadmium exposure and aerial exposure at different temperatures. Fish Sci 89, 633–646 (2023). https://doi.org/10.1007/s12562-023-01704-7

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