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Characterization and expression analysis of chemokine-like receptor 3 gene in rainbow trout Oncorhynchus mykiss

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Abstract

Chemokine-like receptors (CMKLRs) are multi-functional receptors with roles in regulating leukocyte and inflammation. Currently, two members of the CMKLRs, CMKLR1 and CMKLR2, have been found in salmonids, indicating that the CMKLRs had expanded from an ancestor gene in teleost fish. In the present study, the third member of the CMKLRs, defined as CMKLR3, was identified and cloned in rainbow trout. The trout CMKLR3 possessed conserved features of the CMKLR family including seven transmembrane regions, a dynein regulatory complex (DRC) motif, and two cysteine residues, but shared low sequence identities with fish CMKLR1 and CMKLR2 (24‒38 %), which was confirmed by phylogenetic tree analysis. Trout CMKLR3 was highly expressed in body kidney, head kidney, and IgM+ B cells, indicating its functional role in regulating leukocytes. We were able to modulate the expression of trout CMKLR3 in vivo by bacterial and parasitic infections but it remained apathetic to virus infection, and it was also successfully modulated in vitro by peptidoglycan and cytokines (IFN-γ and IL-6). Our results suggest that trout CMKLR3 is regulated in a complex way and has an important regulatory role in inflammatory responses.

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Acknowledgments

This work was supported by the Scottish Funding Council for the MASTS (the Marine Alliance for Science and Technology for Scotland) (grant reference HR09011) and partially by grants from the National Natural Science Foundation of China (31302221, 31272666, and 31470130). ZQ was financially supported by the “Qinglan” project of Jiangsu Province and the overseas training plan for young and middle-aged teachers and principals of colleges and universities in Jiangsu Province, China. We thank Professor Mingxian Chang, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, for her kind comments on the phylogenetic tree analysis.

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland, Yancheng Institute of Technology, Yancheng, Jiangsu, 224051, China

    Zhitao Qi, Qihuan Zhang & Xiuchun Wang

  2. Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen, Aberdeen, AB24 2TZ, UK

    Zhitao Qi, Jason W. Holland & Tiehui Wang

  3. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China

    Qian Gao

  4. Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación Agraria y Alimentaria, Valdeolmos, Madrid, 28130, Spain

    Carolina Tafalla

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  1. Zhitao Qi
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Correspondence to Zhitao Qi.

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Qi, Z., Zhang, Q., Holland, J.W. et al. Characterization and expression analysis of chemokine-like receptor 3 gene in rainbow trout Oncorhynchus mykiss . Fish Sci 82, 613–622 (2016). https://doi.org/10.1007/s12562-016-0997-5

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