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Modulation of gut microbiota associated with abalone Haliotis gigantea by dietary administration of host-derived Pediococcus sp. Ab1

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Abstract

In the present study, we evaluated how dietary administration of host-derived Pediococcus sp. Ab1 has an effect on the abalone gut microbiota using a culture-dependent method and 16S rRNA gene library analysis. The culturable lactic acid bacteria number in the probiotic sample was 105 higher than that in the non-probiotic sample, and we speculate that this significant increase was due to colonization of Ab1 into abalone gut. The result of a culture-dependent method showed that the proportion of Vibrio halioticoli clade, which is known to be a beneficial resident bacterium to abalone, was much higher in the probiotic sample than in the non-probiotic sample. 16S rRNA gene clone sequences revealed that gut microbiota in the probiotic sample was obviously diverse compared to the non-probiotic sample, probably due to improvement of the gut environment by Ab1 colonization. In addition, some beneficial bacteria–like sequences such as V. halioticoli were only found in the probiotic sample. These results suggest that the dietary administration of Ab1 to abalone gut has a great effect on modulation of not only culturable but also unculturable gut microbiota. Our results are useful for future investigations into understanding the effect of probiotics on gut microbiota.

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Acknowledgments

We thank Mr. Atsushi Hamabe at the Owase Aquaculture Center for kindly providing the abalone samples. This work was supported by a grant-in-aid for Scientific Research (no. 21780176) from the JSPS.

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Authors and Affiliations

  1. Graduate School of Bioresources, Mie University, 1577 Kurima-machiya, Tsu, Mie, 514-8507, Japan

    Shunpei Iehata, Miyo Nakano & Reiji Tanaka

  2. Faculty of Fisheries, Kagoshima University, 4-50-20 Shimoarata, Kagoshima, 890-0056, Japan

    Hiroto Maeda

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  1. Shunpei Iehata
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Correspondence to Reiji Tanaka.

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Iehata, S., Nakano, M., Tanaka, R. et al. Modulation of gut microbiota associated with abalone Haliotis gigantea by dietary administration of host-derived Pediococcus sp. Ab1. Fish Sci 80, 323–331 (2014). https://doi.org/10.1007/s12562-013-0691-9

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  • DOI: https://doi.org/10.1007/s12562-013-0691-9

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