Skip to main content
Log in

Effect of seaweed epibiotic bacterium Streptomyces violaceoruber SCH-09 on marine fouling organisms

  • Original Article
  • Chemistry and Biochemistry
  • Published:
Fisheries Science Aims and scope Submit manuscript

Abstract

Seaweed-associated bacteria play a role in helping to protect host organisms from biofouling by producing anti-fouling compounds. In a first step to develop such anti-fouling compounds, we isolated epibiotic bacteria from seaweed and subsequently screen culture extracts for anti-fouling activities. The active epibiotic bacterium studied was isolated from Undaria pinnatifida and identified as Streptomyces violaceoruber based on 16S rDNA sequence analysis. Two active compounds were isolated from the culture extracts by silica gel chromatography and high-performance liquid chromatography, and their structures were analyzed by nuclear magnetic resonance and high-resolution electron ionization mass spectrometry. The compounds were identified as furanone derivatives: 3-octa-1′,3′-dienyl-4-methylfuran-2(5H)-one and 3-octa-1′-enyl-4-methylfuran-2(5H)-one, respectively. These compounds showed anti-fouling activities against the following fouling organisms: zoospores of Ulva pertusa, the diatom Navicula annexa, and the mussel Mytilus edulis, with an EC50 (minimum concentration for 50 % spore inhibition) range of 0.02–0.1 μg/ml. In the acute toxicity tests on juvenile rockfish Shebastes shlegelli, brine shrimp Artemia salina, and microalga Tetraselmis suecica, the two compounds showed LC50 (concentration lethal to 50 % of the spores) ranges of 13.9–118.9 and 14.8–81.9 μg/ml, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Dobretsov SV, Qian P-Y (2002) Effect of bacteria associated with the green alga Ulva reticulata on marine micro- and macrofouling. Biofouling 18:217–228

    Article  Google Scholar 

  2. Dorbetsov S, Dahms H-U, Qian P-Y (2006) Inhibition of biofouling by marine microorganisms and their metabolites. Biofouling 22:43–54

    Article  Google Scholar 

  3. Yebra DM, Kiil S, Dam-Johansen K (2004) Antifouling technology—past, present and future steps towards efficient and environmentally friendly antifouling coatings. Prog Org Coat 50:75–104

    Article  CAS  Google Scholar 

  4. Phillippi AL, O’Conner NJ, Lewis AF, Kim YK (2001) Surface flocking as possible anti-biofoulant. Aquaculture 195:225–238

    Article  Google Scholar 

  5. Folke C, Kautsy N, Troell M (1994) The costs of eutrophication from salmon farming—implications for policy. J Environ Manag 40:173–182

    Google Scholar 

  6. Gollasch S (2002) The importance of ship hull fouling as a vector of species introduction into the North Sea. Biofouling 18:105–121

    Article  Google Scholar 

  7. Davidson IC, Brown CW, Sytsma MD, Ruiz GM (2009) The role of containerships as transfer mechanisms of marine biofouling species. Biofouling 25:645–655

    Article  PubMed  Google Scholar 

  8. Godwin LS (2003) Hull fouling of maritime vessels as a pathway for marine species invasions to the Hawaiian Islands. Biofouling 19:123–131

    Article  PubMed  Google Scholar 

  9. Holt JS (1993) Mechanisms and agronomic aspects of herbicide resistance. Annu Rev Plant Physiol Plant Mol Biol 44:203–229

    Article  CAS  Google Scholar 

  10. Hall LW Jr, Gidding JM, Solomon KR, Balcomb R (1999) An ecological risk assessment for the use of Irgarol 1051 as an algaecide for antifouling paints. Crit Rev Toxicol 29:367–437

    PubMed  CAS  Google Scholar 

  11. Thomas KV, McHugh M, Waldock M (2002) Antifouling paint booster biocides in UK coastal waters: input, occurrence and environmental fate. Sci Total Environ 293:117–127

    Article  PubMed  CAS  Google Scholar 

  12. Armstrong E, Yan L, Boyd KG, Wright PC, Burgess G (2001) The symbiotic role of marine microbes on living surfaces. Hydrobiologia 461:37–40

    Article  Google Scholar 

  13. Keats DW, Knight MA, Pueschel CM (1997) Antifouling effects of epithelial shedding in three crustose coralline algae (Rhodophyta, Corallinales) on a coral reef. J Exp Mar Biol Ecol 213:281–293

    Article  Google Scholar 

  14. Dobretsov SV (1999) Effects of macroalgae and biofilm on settlement of blue mussel (Mytilus edulis L.) larvae. Biofouling 14:153–165

    Article  Google Scholar 

  15. Dobretsov SV, Wahl M (2001) Recruitment preferences of blue mussel spat (Mytilus edulis) for different substrata and microhabitats in the White Sea (Russia). Hydrobiologia 445:27–35

    Article  Google Scholar 

  16. Egan S, James S, Holmström C, Kjelleberg S (2001) Inhibition of algal spore germination by the marine bacterium Pseudoalteromonas tunicata. FEMS Microbiol Ecol 35:67–73

    Article  PubMed  CAS  Google Scholar 

  17. Etoh H, Kondoh T, Yoshioka N, Sugiyama K, Ishikawa H, Tanaka H (2003) 9-oxo-neoprocurcumenol from Curcuma aromatica (Zingiberaceae) as an attachment inhibitor against the blue mussel, Mytulus edulis galloprovincialis. Biosci Biotechnol Biochem 67:911–913

    Article  PubMed  CAS  Google Scholar 

  18. Sidharthan M, Shin HW, Joo JH (2004) Fouling coverage of a green tide alga, Ulva pertusa, on some antifouling test surfaces exposed to Ayagin harbor waters, east coast of South Korea. J Environ Biol 25:39–43

    PubMed  CAS  Google Scholar 

  19. Park NH, Hong YK, Cho JY (2006) Screening anti-inflammatory actinomyces isolated from seaweeds and marine sediments. J Kor Fish Soc 39:333–337

    Google Scholar 

  20. Guillard RL, Ryther JH (1962) Studies on marine planktonic diatoms I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran. Can J Microbiol 8:229–239

    Article  PubMed  CAS  Google Scholar 

  21. Clare AS, Rittschof D, Gerhart DJ, Maki JS (1992) Molecular approaches to nontoxic antifouling. Invertebr Reprod Dev 22:67–76

    Article  CAS  Google Scholar 

  22. Rao JV, Kavitha P, Jakka NM, Sridhar V, Usman PK (2007) Toxicity of Organophosphates on Morphology and Locomotor Behavior in Brine Shrimp, Artemia salina. Arch Environ Contam Toxicol 53:227–232

    Article  Google Scholar 

  23. United States Environmental Protection Agency (US-EPA) (2002) Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms, 5th edn. Publication EPA-821-R-02-012. US EPA, Washington, DC

    Google Scholar 

  24. Fusetani N (2011) Antifouling marine natural products. Nat Prod Rep 28:400–410

    Article  PubMed  CAS  Google Scholar 

  25. Cho JY, Kwon HC, Williams PG, Kauffman CA, Jensen PR, Fenical W (2006) Actinofuranones A and B, polyketides from a marine-derived bacterium related to the genus Streptomyces (Actinomycetales). J Nat Prod 69:425–428

    Article  PubMed  CAS  Google Scholar 

  26. Paulitz T, Nowak-Thompson B, Gamard P, Tsang E, Loper J (2000) A novel antifungal furanone from Pseudomonas aureofaciens, a biocontrol agent of fungal plant pathogens. J Chem Ecol 26:1515–1524

    Article  CAS  Google Scholar 

  27. Kim C, Kim J, Park H-Y, Park H-J, Lee J-H, Kim C-K, Yoon J (2008) Furanone derivatives as quorum-sensing antagonists of Pseudomonas aeruginosa. Appl Microbiol Biotechnol 80:37–47

    Article  PubMed  CAS  Google Scholar 

  28. Xu Y, He H, Schulz S, Liu X, Fusetani N, Xiong H, Xiao X, Qian P-Y (2010) Potent antifouling compounds produced by marine Streptomyces. Bioresource Technol 101:1331–1336

    Article  CAS  Google Scholar 

  29. Wang Y, Gloer JB, Scott JA, Malloch D (1993) Appenolides A-C: three new antifungal furanones from the coprophilous fungus Podospora appendiculata. J Nat Prod 56:341–344

    Article  PubMed  CAS  Google Scholar 

  30. Corre S, Prieur D (1990) Density and morphology of epiphytic bacteria on the kelp Laminaria digitala. Bot Mar 33:515–523

    Google Scholar 

  31. Prieur D, Gaill F, Corre S (1993) Complex epibiotic bacterial communities on marine organisms: fouling or interactions? In: Trends in microbial ecology: Proc 6th Int Symp Microbial Ecology 1992. Spanish Society for Microbiology, Barcelona, pp 207–212

  32. Singh RP, Mantri VA, Reddy CRK, Jha B (2011) Isolation of seaweed-associated bacteria and their morphogenesis-inducing capability in axenic cultures of the green alga Ulva fasciata. Aquat Biol 12:13–21

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST) (NRF-C1ABA001-2012-0029960).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ji Young Cho.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hong, YK., Cho, J.Y. Effect of seaweed epibiotic bacterium Streptomyces violaceoruber SCH-09 on marine fouling organisms. Fish Sci 79, 469–475 (2013). https://doi.org/10.1007/s12562-013-0604-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12562-013-0604-y

Keywords

Navigation