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Expression profiles of interferon gamma genes in response to immunostimulants and alloantigen in ginbuna crucian carp Carassius auratus langsdorfii

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Abstract

Interferon gamma (IFNγ), a Th1-derived cytokine is one of the key molecules inducing cell-mediated immune responses in mammals. The expression of 2 distinct IFNγ (ifng1 and ifng2) and IFNγrel (ifngrel) genes was examined in kidney leukocytes from clonal ginbuna crucian carp Carassius auratus langsdorfii. The expression of IFNγ and IFNγrel genes was induced in kidney leukocytes by stimulation with lipopolysaccharide or phytohemagglutinin in a dose-dependent manner. The expression levels of IFNγ and IFNγrel genes in kidney leukocytes from allo-sensitized fish cocultured with allogeneic cells were higher than those from fish cocultured with isogeneic cells. The highest expression of ifng1 was observed at day 1, whereas that of ifng2 and ifngrel was detected at day 2 after cocultivation with allogeneic cells. However, the expression pattern of ifng2 and ifngrel in kidney leukocytes from allo-sensitized fish by scale-grafting was similar to those from non-sensitized fish. These results indicate that ifng1 is a major isoform of IFNγ related to antigen-specific cell-mediated immunity (CMI), and will be a useful marker to evaluate induction of CMI in ginbuna crucian carp. In addition, IFNγ would be a crucial type-II interferon compared to IFNγrel, relevant to cell-mediated immunity in fish as in mammals.

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Acknowledgments

This research was supported by the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Young Scientists (B) from Japan Society for the Promotion of Science (JSPS).

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Authors and Affiliations

  1. Faculty of Fisheries, Kagoshima University, Kagoshima, 890-0056, Japan

    Kyosuke Araki, Masatoshi Yamasaki & Atsushi Yamamoto

  2. College of Bioresource Sciences, Nihon University, Kanagawa, 252-8510, Japan

    Fumio Takizawa, Mariko Esumi, Tadaaki Moritomo & Teruyuki Nakanishi

  3. Inland Station, National Research Institute of Aquaculture, Fisheries Research Agency, Mie, 519-0423, Japan

    Mitsuru Ototake

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  1. Kyosuke Araki
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Correspondence to Teruyuki Nakanishi.

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Araki, K., Takizawa, F., Yamasaki, M. et al. Expression profiles of interferon gamma genes in response to immunostimulants and alloantigen in ginbuna crucian carp Carassius auratus langsdorfii . Fish Sci 79, 213–220 (2013). https://doi.org/10.1007/s12562-012-0590-5

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  • DOI: https://doi.org/10.1007/s12562-012-0590-5

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