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Characteristics of a self-assembled fibrillar gel prepared from red stingray collagen

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  • Chemistry and Biochemistry
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Abstract

A translucent collagen gel was formed from a transparent acidic solution of red stingray collagen by adjusting to physiological ionic strength and pH in phosphate buffer and then incubating at 25–37°C. During fibril formation from red stingray collagen, the turbidity increased when the NaCl concentration was increased at constant pH and the rate of fibril formation was accelerated by higher pH or lower NaCl concentration. The T m of red stingray collagen fibrillar gel was estimated as 44.3 ± 3.5°C, which was higher than that of the collagen solution, 33.2°C. In addition, red stingray collagen gel maintained its shape without melting and was suitable for culture of mouse stromal cells at 37°C.

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Acknowledgments

The authors thank Cell Resource Center for Biomedical Research Institute of Development, Aging, and Cancer, Tohoku University, for providing of mouse stromal cells (FLS 5). This work was supported in part by a Grant-in-Aid for Scientific Research from Nagasaki University, Japan.

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Authors and Affiliations

  1. Graduate School of Science and Technology, Nagasaki University, 1-14 Bunkyo, Nagasaki, 852-8521, Japan

    Inwoo Bae & Asami Yoshida

  2. Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo, Nagasaki, 852-8521, Japan

    Kiyoshi Osatomi, Atsuko Yamaguchi, Katsuyasu Tachibana, Tatsuya Oda & Kenji Hara

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  1. Inwoo Bae
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  2. Kiyoshi Osatomi
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  3. Asami Yoshida
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  4. Atsuko Yamaguchi
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  5. Katsuyasu Tachibana
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  6. Tatsuya Oda
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  7. Kenji Hara
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Correspondence to Kenji Hara.

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Bae, I., Osatomi, K., Yoshida, A. et al. Characteristics of a self-assembled fibrillar gel prepared from red stingray collagen. Fish Sci 75, 765–770 (2009). https://doi.org/10.1007/s12562-009-0085-1

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  • DOI: https://doi.org/10.1007/s12562-009-0085-1

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