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Molecular Detection of Adenoviruses in Lakes and Rivers of Goiânia, Goiás, Brazil

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Abstract

Adenoviruses are a highly important public health issue, since they are among the most persistent and ubiquitous viruses present in water and associated with a variety of clinical manifestations. The aim of this study was to use molecular techniques for the detection of adenovirus in public and recreational water supplies in Goiânia, Brazil. From December 2007 to November 2008, 54 water samples were collected in 5 different sites in 2 lakes and 2 rivers of the city. The samples were filtered in a positively charged nylon membrane, and the DNA was extracted using the phenol–chloroform–isoamyl alcohol method. Semi-nested PCR was used to detect adenovirus DNA, and sequence analysis of the semi-nested PCR products was performed to identify the recovered viruses. Adenovirus DNA was detected in 43% (24 of 54) of samples collected. Considering all examined sites, MP1 presented the highest occurrence of adenovirus (6 positive from 10 collected samples), followed by MP2 (3 positive from 6 collected samples), JL (10 positive from 21 collected samples), VB (3 positive from 9 collected samples), and BB (2 positive from 8 collected samples), respectively. The methodology employed proved to be feasible, fast, low-cost, and suitable to be used as screening approach on adenovirus detection in water for public sanitation companies.

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Acknowledgments

The authors would like to thank Núcleo de Pesquisas Replicon from Pontifical Catholic University of Goiás and LaGene, Laboratório de Citogenética Humana e Genética Molecular from Secretary of Health of Goiania-Goiás for providing the molecular sequencing of samples and research support.

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Authors and Affiliations

  1. Laboratório de Diagnóstico Genético e Molecular, Instituto de Ciências Biológicas II, Universidade Federal de Goiás, Campus Samambaia, Caixa Postal 131, Goiânia, GO, 74001-970, Brazil

    Hugo D. Silva, Ludimila A. C. Wosnjuk & Carlos E. Anunciação

  2. Núcleo de Pesquisas em Agentes Emergentes e Re-emergentes, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Caixa Postal 12911, Setor Leste Vila Nova, Goiânia, GO, 74643-970, Brazil

    Sônia F. O. Santos & Marco T. A. Garcíazapata

  3. Laboratório de Genética Molecular e Citogenética, Instituto de Ciências Biológicas I, Universidade Federal de Goiás, Campus Samambaia, Caixa Postal 131, Goiânia, GO, 74001-970, Brazil

    Cesar A. S. T. Vilanova-Costa, Flávia C. Pereira & Elisângela P. Silveira-Lacerda

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  1. Hugo D. Silva
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  2. Ludimila A. C. Wosnjuk
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  3. Sônia F. O. Santos
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  4. Cesar A. S. T. Vilanova-Costa
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  5. Flávia C. Pereira
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  6. Elisângela P. Silveira-Lacerda
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  7. Marco T. A. Garcíazapata
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  8. Carlos E. Anunciação
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Correspondence to Carlos E. Anunciação.

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Silva, H.D., Wosnjuk, L.A.C., Santos, S.F.O. et al. Molecular Detection of Adenoviruses in Lakes and Rivers of Goiânia, Goiás, Brazil. Food Environ Virol 2, 35–40 (2010). https://doi.org/10.1007/s12560-009-9023-8

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  • DOI: https://doi.org/10.1007/s12560-009-9023-8

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