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Natural occurrence of alternariol and alternariol monomethyl ether in soya beans

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Abstract

The natural occurrence of alternariol (AOH) and alternariol monomethyl ether (AME) in soya beans harvested in Argentina was evaluated. Both toxins were simultaneously detected by using HPLC analysis coupled with a solid phase extraction column clean-up. Characteristics of this in-house method such as accuracy, precision and detection and quantification limits were defined by means of recovery test with spiked soya bean samples. Out of 50 soya bean samples, 60% showed contamination with the mycotoxins analyzed; among them, 16% were only contaminated with AOH and 14% just with AME. Fifteen of the positive samples showed co-occurrence of both mycotoxins analyzed. AOH was detected in concentrations ranging from 25 to 211 ng/g, whereas AME was found in concentrations ranging from 62 to 1,153 ng/g. Although a limited number of samples were evaluated, this is the first report on the natural occurrence of Alternaria toxins in soya beans and is relevant from the point of view of animal public health.

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Acknowledgments

This work was supported by the Secretaría de Ciencia y Técnica, Universidad Nacional de Río Cuarto (SECyT-UNRC). M.S.O. is a fellow of CONICET and M.L.R., G.G.B. and S.C., are members of the Research Career of CONICET.

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Authors and Affiliations

  1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Fco-Qcas y Naturales, Universidad Nacional de Rio Cuarto, Ruta 36 Km 601, 5800, Río Cuarto, Córdoba, Argentina

    M. S. Oviedo, G. G. Barros, S. N. Chulze & M. L. Ramirez

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  1. M. S. Oviedo
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  2. G. G. Barros
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  3. S. N. Chulze
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  4. M. L. Ramirez
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Correspondence to M. L. Ramirez.

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Oviedo, M.S., Barros, G.G., Chulze, S.N. et al. Natural occurrence of alternariol and alternariol monomethyl ether in soya beans. Mycotoxin Res 28, 169–174 (2012). https://doi.org/10.1007/s12550-012-0132-0

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  • DOI: https://doi.org/10.1007/s12550-012-0132-0

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