Abstract
Recently, additive manufacturing is one of the most focused research topics due to its explosive development, especially in manufacturing engineering and medical science. In order to build 3D complex scaffolds with multi-biomaterials for clinical application, a new 3D multi-nozzle system with dual-mode drives, i.e. ejection and extrusion was developed. In this paper, much effort was made to gain fine control of droplet and excellent coordination during fabrication. Specifically, the parameters that influence the size and stability of droplet most was intensively studied. Considering that the biomaterials used in the future may have much difference in properties, the combination of parameters was investigated to facilitate the settings for certain-sized droplets, which are potentially eligible for bio-printing. The dispensing nozzles can work well both in independent and convergent mode, which can be freely switched. Outstanding to the most currently used 3D bio-printing techniques, this system can fabricate scaffolds with multimaterials of both low viscosity (by pneumatic dispensing) and high viscosity (through motor extrusion). It is highly expected that this system can satisfy clinical application in the near future.
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Abbreviations
- θ :
-
contact angle
- D a :
-
the actual diameter of droplet
- D c :
-
cross-sectional diameter
- F c :
-
conversion factor of Da and Dc
- R c :
-
cross-sectional radius (= Dc/2)
- R o :
-
observed radius
- V d :
-
the actual volume of droplet
References
Ringeisen, B. R., Spargo, B. J., and Wu, P. K., “Cell and Organ Printing,” Springer Science & Business Media, 2010.
Yoo, D.-J., “Recent Trends and Challenges in Computer-Aided Design of Additive Manufacturing-Based Biomimetic Scaffolds and Bioartificial Organs,” Int. J. Precis. Eng. Manuf., Vol. 15, No. 10, pp. 2205–2217, 2014.
Gibson, I., Rosen, D. W., and Stucker, B., “Additive Manufacturing Technologies,” Springer, 2010.
Wong, K. V. and Hernandez, A., “A Review of Additive Manufacturing,” ISRN Mechanical Engineering, Vol. 2012, Article ID: 208760, 2012.
Guo, N. and Leu, M. C., “Additive Manufacturing: Technology,” Applications and Research Needs, Frontiers of Mechanical Engineering, Vol. 8, No. 3, pp. 215–243, 2013.
Bose, S., Vahabzadeh, S., and Bandyopadhyay, A., “Bone Tissue Engineering Using 3D Printing,” Materials Today, Vol. 16, No. 12, pp. 496–504, 2013.
Chern, M.-J., Yang, L.-Y., Shen, Y.-K., and Hung, J.-H., “3D Scaffold with PCL Combined Biomedical Ceramic Materials for Bone Tissue Regeneration,” Int. J. Precis. Eng. Manuf., Vol. 14, No. 12, pp. 2201–2207, 2013.
Pati, F., Song, T.-H., Rijal, G., Jang, J., Kim, S. W., and Cho, D.-W., “Ornamenting 3D Printed Scaffolds with Cell-Laid Extracellular Matrix for Bone Tissue Regeneration,” Biomaterials, Vol. 37, pp. 230–241, 2015.
Suárez-González, D., Lee, J. S., Diggs, A., Lu, Y., Nemke, B., et al., “Controlled Multiple Growth Factor Delivery from Bone Tissue Engineering Scaffolds via Designed Affinity,” Tissue Engineering Part A, Vol. 20, Nos. 15-16, pp. 2077–2087, 2013.
Klein, T. J., Rizzi, S. C., Reichert, J. C., Georgi, N., Malda, J., et al., “Strategies for Zonal Cartilage Repair Using Hydrogels,” Macromolecular Bioscience, Vol. 9, No. 11, pp. 1049–1058, 2009.
Chang, L., Thian, E., Sun, J., Fuh, J., Hong, G., et al., “Fabrication of Functionally Graded Hydroxyapatite/Titanium Oxide Coating via Drop-on-Demand Technique,” Nano LIFE, Vol. 2, No. 1, Paper No. 1250009, 2012.
Lee, J.-S., Hong, J. M., Jung, J. W., Shim, J.-H., Oh, J.-H., and Cho, D.-W., “3D Printing of Composite Tissue with Complex Shape Applied to Ear Regeneration,” Biofabrication, Vol. 6, No. 2, Paper No. 024103, 2014.
Kalita, S. J., Bose, S., Hosick, H. L., and Bandyopadhyay, A., “Development of Controlled Porosity Polymer-Ceramic Composite Scaffolds via Fused Deposition Modeling,” Materials Science and Engineering: C, Vol. 23, No. 5, pp. 611–620, 2003.
Williams, J. M., Adewunmi, A., Schek, R. M., Flanagan, C. L., Krebsbach, P. H., et al., “Bone Tissue Engineering Using Polycaprolactone Scaffolds Fabricated via Selective Laser Sintering,” Biomaterials, Vol. 26, No. 23, pp. 4817–4827, 2005.
Singh, M., Haverinen, H. M., Dhagat, P., and Jabbour, G. E., “Inkjet Printing-Process and Its Applications,” Advanced Materials, Vol. 22, No. 6, pp. 673–685, 2010.
Schiele, N. R., Corr, D. T., Huang, Y., Raof, N. A., Xie, Y., and Chrisey, D. B., “Laser-Based Direct-Write Techniques for Cell Printing,” Biofabrication, Vol. 2, No. 3, Paper No. 032001, 2010.
Huang, W., Zhang, X., Wu, Q., and Wu, B., “Fabrication of Ha/ß-TCP Scaffolds Based on Micro-Syringe Extrusion System,” Rapid Prototyping Journal, Vol. 19, No. 5, pp. 319–326, 2013.
Ren, X., Zhang, Q., Liu, K., Li, H.-l., and Zhou, J. G., “Modeling of Pneumatic Valve Dispenser for Printing Viscous Biomaterials in Additive Manufacturing,” Rapid Prototyping Journal, Vol. 20, No. 6, pp. 434–443, 2014.
Blandino, A., Macias, M., and Cantero, D., “Formation of Calcium Alginate Gel Capsules: Influence of Sodium Alginate and CaCl2 Concentration on Gelation Kinetics,” Journal of Bioscience and Bioengineering, Vol. 88, No. 6, pp. 686–689, 1999.
Zactiti, E. M. and Kieckbusch, T. G., “Release of Potassium Sorbate from Active Films of Sodium Alginate Crosslinked with Calcium Chloride,” Packaging Technology and Science, Vol. 22, No. 6, pp. 349–358, 2009.
Zhou, K., Zhang, X., Chen, Z., Shi, L., and Li, W., “Preparation and Characterization of Hydroxyapatite-Sodium Alginate Scaffolds by Extrusion Freeforming,” Ceramics International, Vol. 41, No. 10, pp. 14029–14034, 2015.
Klüsener, O., “The Injection Process in Compressorless Diesel Engines,” VDI Z, Vol. 77, No. 7, 1933.
Ohnesorge, W. V., “Formation of Drops by Nozzles and the Breakup of Liquid Jets,” Journal of Applied Mathematics and Mechanics, Vol. 16, No. 4, pp. 355–358, 1936.
Lefebvre, A., “Atomization and Sprays,” CRC Press, 1988.
Yeong, W.-Y., Chua, C.-K., Leong, K.-F., and Chandrasekaran, M., “Rapid Prototyping in Tissue Engineering: Challenges and Potential,” Trends in Biotechnology, Vol. 22, No. 12, pp. 643–652, 2004.
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Chen, Z., Zhang, X., Chen, P. et al. 3D multi-nozzle system with dual drives highly potential for 3D complex scaffolds with multi-biomaterials. Int. J. Precis. Eng. Manuf. 18, 755–761 (2017). https://doi.org/10.1007/s12541-017-0090-8
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DOI: https://doi.org/10.1007/s12541-017-0090-8