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In vitro cytotoxicity effects of polycyclic aromatic hydrocarbons (PAHs) associated with PM10 during the Middle Eastern Dust (MED) storms in Ahvaz

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Abstract

This study aimed to investigate the cytotoxic effects of PAHs extracts by PM10 from Ahvaz (Iran) during normal and dusty days from December 2012 to June 2015, using the A549 cell line (adenocarcinoma human alveolar basal epithelial cells) and lactase dehydrogenase (LDH) ELISA test. Also, the source of PM10 will be evaluated using the HYSPLIT Air Q model application. PAHs concentrations associated with PM10 were evaluated by chromatography-mass spectrometry (GC–MS). Based on the obtained results, PM10 on both dusty and normal days caused cytotoxicity in the control group cells. According to t-test findings, the value of cytotoxicity in normal days was different from dust event days. Similarly, the tests of the normal days illustrated more extreme-level cytotoxicity than dust storm days at the treated dosage. Higher cytotoxicity was observed by increasing the incubation time of cells, which was supported by the ANOVA test. There is a significant relationship between different incubation times based on the ANOVA. Due to their ring structure, the PAHs compounds can exacerbate the toxicity in the samples at longer times.

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Acknowledgements

The authors would like to appreciate Ahvaz Jundishapur University of Medical Sciences (ETRC- 9330), Neyshabur University of Medical Sciences, for financing roles, respectively.

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Authors and Affiliations

  1. Department of Environmental Health Engineering, Neyshabur University of Medical Sciences, Neyshabur, Iran

    Abolfazl Naimabadi & Ali Akbar Mohammadi

  2. Social Determinants of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

    Mohammad Shirmardi

  3. Environmental Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

    Mohammad Shirmardi

  4. Department of Environmental Health Engineering, School of Public Health, Babol University of Medical Sciences, Babol, Iran

    Mohammad Shirmardi

  5. Environmental Technologies Research Center (ETRC), Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Gholamreza Goudarzi

  6. Department of Environmental Health Engineering, Health Faculty, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Gholamreza Goudarzi

  7. Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Ata Ghadiri

  8. Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Ata Ghadiri

  9. Department of Environmental Health Engineering, School of Public Health, Tehran University of MedicalSciences, Tehran, Iran

    Vahide Oskoei

  10. Environmental and Food Hygiene Laboratory (LIAA) of Department of Medical, Surgical Sciences and Advanced Technologies “G.F. Ingrassia”, University of Catania, Catania, Italy

    Gea Oliveri Conti & Margherita Ferrante

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  1. Abolfazl Naimabadi
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  2. Mohammad Shirmardi
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  3. Gholamreza Goudarzi
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  4. Ata Ghadiri
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  5. Vahide Oskoei
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  6. Ali Akbar Mohammadi
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  7. Gea Oliveri Conti
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  8. Margherita Ferrante
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Correspondence to Ali Akbar Mohammadi.

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Naimabadi, A., Shirmardi, M., Goudarzi, G. et al. In vitro cytotoxicity effects of polycyclic aromatic hydrocarbons (PAHs) associated with PM10 during the Middle Eastern Dust (MED) storms in Ahvaz. Arab J Geosci 15, 531 (2022). https://doi.org/10.1007/s12517-022-09834-5

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  • DOI: https://doi.org/10.1007/s12517-022-09834-5

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