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Differential down-regulation of zeaxanthin epoxidation in two rice (Oryza sativa L.) cultivars with different chilling sensitivities

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Abstract

When the leaf segments of rice (Oryza sativa L.) plants were subjected to chilling in the moderate light, zeaxanthin (Zx) formation was faster in a chilling-tolerant Dongjin-byeo (DJ) than in a chilling-sensitive IR841. Although the rate of Zx formation was accelerated by the treatment of 5 mM salicylaldoxime, an inhibitor of Zx epoxidase (ZE), there was almost no changes in DJ. A similar result was observed when leaf segments were treated with 50 mM sodium fluoride, a potent inhibitor of chloroplast phosphatase. The slow Zx epoxidation in IR841 during light-chilling was confirmed in leaf segments treated with 10 mM dithiothreitol, an inhibitor of violaxanthin de-epoxidase (VDE). However, the differences between the two cultivars were not observed at 25oC. These results suggest that compared with IR841 the higher rate of Zx formation in DJ is not due to the higher VDE activity in DJ but is due to more rapid down-regulation of ZE in DJ, possibly by its phosphorylation. Compared with DJ, IR841 accumulated more superoxide with PSI inactivation during light-chilling, which eliminates the possibility of increased ZE down-regulation in DJ leaves by photo-oxidation. In vitro study with alkaline phosphatase supports the idea of down-regulation of ZE by phosphorylation under light-chilling condition. We propose that this reversible down-regulation of Zx epoxidation possibly by the phosphorylation of ZE is an important regulation mechanism of violaxanthin cycle that confers chilling tolerance of a rice cultivar under chilling stress in the light with moderate intensities.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Integrated Biological Science, Department of Molecular Biology, Pusan National University, Busan, 46241, Korea

    Ho-Seung Kim, Minh Hien Hoang, Young Ah Jeon, Guangxi Wu & Choon-Hwan Lee

  2. Algal Biotechnology Department, Institute of Biotechnology, Vietnamese Academy of Science and Technology, Hanoi, Vietnam

    Minh Hien Hoang

  3. National Agrobiodiversity Center, National Academy of Agricultural Science, Wanju-gun, Jeollabuk-do, 54875, Korea

    Young Ah Jeon

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  1. Ho-Seung Kim
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  2. Minh Hien Hoang
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  4. Guangxi Wu
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  5. Choon-Hwan Lee
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Correspondence to Choon-Hwan Lee.

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Kim, HS., Hoang, M.H., Jeon, Y.A. et al. Differential down-regulation of zeaxanthin epoxidation in two rice (Oryza sativa L.) cultivars with different chilling sensitivities. J. Plant Biol. 60, 413–422 (2017). https://doi.org/10.1007/s12374-016-0483-8

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