Skip to main content
SpringerLink
Log in

The Community of Arbuscular Mycorrhizal Fungi in Sugarcane Rhizosphere Soils in Guangxi, China

  • Research Article
  • Published:
Sugar Tech Aims and scope Submit manuscript

Abstract

In order to analyze the community of the arbuscular mycorrhizal fungi (AMF) in the rhizosphere soil and roots of sugarcane in Guangxi, China, this study analyzed the 18S rDNA gene sequences, soil physicochemical properties, root mycorrhizal colonization, and correlation of soil factors in three types of soil, lateritic red soil, latosol soil, and red soil. The results showed that a total of 24 species were identified, belonging to 12 genera and 7 families, among which the family Glomeraceae contained the most species with 5 genera and 10 species, followed by Diversisporaceae (1 genus and 2 species), Acaulosporaceae (1 genus and 1 species), Gigasporaceae (2 genera and 2 species), Claroideoglomeraceae (1 genus and 5 species), Archaeosporaceae (1 genus and 2 species), and Paraglomaceae (1 genus and 2 species). The dominant family and genus in the three types of soil were Glomeraceae and Rhizophagus, respectively. The species richness and Shannon diversity index of AMF were the highest in red soil, while the lowest in latosol soil. However, eleven species of AMF were identified in the roots of sugarcane from the three soil types. At the taxonomic level, the genus Glomus and Acaulospora were found in sugarcane roots from the three types of soil, while the genera Claroideoglomus, Acaulospora, and Paraglomus were only found in the roots from lateritic red soil. The genus Diversispora was found only in the roots from lateritic red soil and latosol soil. The genus Glomus is dominant in all the root samples. The physicochemical properties of the three soil types were not significantly correlated with the species richness of AMF. The root colonization rate of AMF was positively correlated with soil pH, while negatively correlated with the organic matter, total nitrogen, and available phosphorus of soils except for the organic matter content of lateritic red soil. These findings suggest that sugarcane roots have a relatively diverse AMF community under natural conditions, which may play an important ecological role in the growth of sugarcane.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25 (17): 3389–3402.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ambrosano, E.J., R. Azcón, H. Cantarella, G.M.B. Ambrosano, E.A. Schammass, T. Muraoka, P.C.O. Trivelin, F. Rossi, N. Guirado, M.R.G. Ungaro, and J.R.S. Teramoto. 2010. Crop rotation biomass and arbuscular mycorrhizal fungi effects on sugarcane. Scientia Agricola 67: 692–701.

    Article  Google Scholar 

  • Barker, S.J., and D. Tagu. 2000. The roles of auxins and cytokinins in mycorrhizal symbioses. J Plant Growth Regulation 19: 144–154.

    Article  CAS  Google Scholar 

  • Biermann, B., and R.G. Linderman. 1981. Quantifying vercular-arbuscular mycorrhizas: A proposed method towards standardization. New Phytologists 87: 63–67.

    Article  Google Scholar 

  • Bigott, A.F., J.W. Hoy, and L.M. Fultz. 2019. Soil properties, microbial communities, and sugarcane yield in paired fifields with short- or long-term sugarcane cultivation histories. Applied Soil Ecology 142: 166–176.

    Article  Google Scholar 

  • Cahyani, V.R., D. Rastikawati, N. Yuniardi, J. Syamsiyah, and Suntoro. 2017. Mycorrhizal diversity in therhizosphere of sugarcane and grass on different soil types. Journal Physics Conference Series 909 (1): 012091.

    Article  Google Scholar 

  • Chen, T.S., S. Li, J.L. Zhang, X.J. Qin, H.J. Liu, Y.M. Tan, Z.N. Yuan, and Y.R. Li. 2011. Research on the formation of arbuscular mycorrhizae in sugarcane. Southwest China Journal of Agriculture Sciences 24 (5): 1757–1760.

    Google Scholar 

  • Chen, T.S., J.L. Zhang, S. Li, X.J. Qin, Q. Wang, C.Q. Wang, S.J. Wu, Y.M. Tan, R.Z. Yang, and Y.R. Li. 2012. Diversity of arbuscular mycorrhizal fungi in sugarcane fields of Southeastern Guangxi. Journal of Southern Agriculture 43 (1): 57–61.

    Google Scholar 

  • Chen, T.S., S. Li, W.Z. Wang, Y. Xing, J.L. Zhang, Y.Y. Huang, W.X. Lu, and Y.M. Tan. 2013. Influence of inoculation of arbuscular mycorrhizal fungi on sugarcane grown in field. Sugarcane and Canesugar 3: 6–10.

    Google Scholar 

  • Chen, T.S., J.L. Zhang, and Y.Y. Long. 2015. Morphological spectrum of AM fungi in rhizosphere of sugarcane in Guangxi. Nanning: Guangxi Science and Technology Press.

    Google Scholar 

  • Cobb, A.B., G.T. Wilson, C.L. Goad, S.R. Bean, R.C. Kaufman, T.J. Herald, and J.D. Wilson. 2016. The role of arbuscular mycorrhizal fungi in grain production and nutrition of sorghum genotypes: Enhancing sustainability through plant-microbial partnership. Agricult, Ecosyst Environ 233: 432–440.

    Article  Google Scholar 

  • Feng, H.Y., G. Feng, J.G. Wang, and X.L. Li. 2003. Regulation of P status in host plant on alkaline phosphatase (ALP) activity in intraradical hyphae and development of extraradical hyphae of AM Fungi. Mycosystema 22 (4): 589–598.

    CAS  Google Scholar 

  • Finlay, R.D. 2008. Ecological aspects of mycorrhizal symbiosis: With special emphasis on the functional diversity of interactions involving the extraradical mycelium. Journal of Experimental Botany 59: 1115–1126.

    Article  CAS  PubMed  Google Scholar 

  • Gai, J.P., G. Feng, and X.L. Li. 2004. Diversity of arbuscular mycorrhizal fungi in field soils from North China. Biodiversity Science 12 (4): 435–440.

    Article  Google Scholar 

  • Gao, A.X., and X.L. He. 2007. Ecological study on AM fungi around roots of medicinal plants in the middle area of Hebei Province. Agriculture Research in the Arid Areas 25 (3): 196–202.

    Google Scholar 

  • He, X.L., C.M. Bai, and L.L. Zhao. 2008. Spatial distribution of arbuscular mycorrhizal fungi in Astragalus adsurgens root-zone soil in Mu Us sand land. Chinese Journal of Applied Ecology 19 (12): 2711–2715.

    CAS  PubMed  Google Scholar 

  • Javot, H., N. Pumplin, and M.J. Harrison. 2007. Phosphate in the arbuscular mycorrhizal symbiosis: Transport properties and regulatory roles. Plant, Cell and Environment 30: 310–322.

    Article  CAS  PubMed  Google Scholar 

  • Jeffries, P., S. Gianinazzi, S. Perotto, and K. Turnau. 2003. The contribution of arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility. Biology and Fertility of Soils 37: 1–16.

    Article  Google Scholar 

  • Karpouzas, D.G., E. Papadopoulou, I. Ipsilantis, I. Friedel, I. Petric, N. Udikovic-Kolic, S. Djuric, E. Kandeler, U. Menkisssoglu-Sprioudi, and F. Martin-Laurent. 2014. Effects of nicosulfuron on the abundance and diversity of arbuscular mycorrhizal fungi used as indicators of pesticide soil microbial toxicity. Ecological Indicators 39: 44–53.

    Article  CAS  Google Scholar 

  • Lee, J., S. Lee, and J.P.W. Young. 2008. Improved PCR primers for the detection and identification of arbuscular mycorrhizal fungi. FEMS Microbiology Ecology 65 (2): 339–349.

    Article  CAS  PubMed  Google Scholar 

  • Li, Q.W., Z.Y. Chen, and H. Liang. 2000. Modern sugarcane improvement technology. Guangzhou, China: South China University of Technology Press.

    Google Scholar 

  • Liao, N., J.L. Zhang, D.P. Li, Q. Wang, Y.Y. Long, Y.M. Tan, S. Li, Z.J. Lu, and T.S. Chen. 2016. Method to stain endophytic fungi in sugarcane roots. Southwest China J Agricult Sci 29 (4): 814–819.

    Google Scholar 

  • Lovelock, C.E., K. Andersen, and J.B. Morton. 2003. Arbuscular mycorrhizal communities in tropical forests are affected by host tree species and environment. Oecologia 135 (2): 268–279.

    Article  ADS  PubMed  Google Scholar 

  • Marin, M., M. Ybarra, A. Fé, and L. García-Férriz. 2002. Effect of arbuscular mycorrhizal fungi and pesticides on Cynara cardunculus growth. Agric and Food Sci in Finland 11 (3): 245–251.

    Article  CAS  Google Scholar 

  • Michelle, S.S., and P.J. David. 2004. Phosphorus and intraspecific density alter plant responses to arbuscular mycorrhizas. Plant and Soil 264: 335–348.

    Article  Google Scholar 

  • Mickan, B.S., L.K. Abbott, K. Stefanova, and Z.M. Solaiman. 2016. Interactions between biochar and mycorrhizal fungi in a water-stressed agricultural soil. Mycorrhiza 26: 565–574.

    Article  CAS  PubMed  Google Scholar 

  • Nasim, G., A. Ali, A. Munawar, and R. Bajwa. 2008. Seasonal dynamics of AM fungi in sugarcane (Saccharum officinarum L. Cv. SPF-213) in relation to red rot (Colletotrichum falcatum) disease from Punjab Pakistan. Pakistan Journal of Botany 40: 2587–2600.

    Google Scholar 

  • Reis, V.M., M.A. de Paula, and J. Döbereiner. 1999. Occurrence of arbuscular mycorrhizae and bacterium Acetobacter diazotrophicus in sugarcane. Pesqui Agropecu Bras 34: 1933–1941.

    Article  Google Scholar 

  • Ren, Z., J.W. Wand, Y.J. Fend, Y. Ruan, and J.N. Lai. 2011. Species diversity of AMF community colonized in maize roots by clone library. Journal of Maize Science 19 (5): 19–24.

    Google Scholar 

  • Rillig, M.C., and D.L. Mummey. 2006. Mycorrhizas and soil structure. New Phytologists 171: 41–53.

    Article  CAS  Google Scholar 

  • Rokni, N., M.E. Goltapeh, and A. Alizadeh. 2010. Some new recorded species of arbuscular mycorrhizal fungi associated with sugarcane crop in Iran. Agriculture Technology 6: 67–78.

    Google Scholar 

  • Schloss, P.D., S.L. Westcott, T. Ryabin, J.R. Hall, M. Hartmann, E.B. Hollister, R.A. Lesniewski, B.B. Oakley, D.H. Parks, C.J. Robinson, J.W. Sahl, B. Stres, G.G. Thallinger, D.J.V. Horn, and C.F. Weberla. 2009. Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities. Applied and Environment Microbiology 75 (23): 7537–7541.

    Article  ADS  CAS  Google Scholar 

  • Schloter, M., P. Nannipieri, S.J. Sørensen, and J.D. van Elsas. 2018. Microbial indicators for soil quality. Biol and Fertility of Soils 54: 1–10.

    Article  CAS  Google Scholar 

  • Schwarzott, D., and A. Schüβler. 2001. A simple and reliable method for SSU rRNA gene DNA extraction, amplification, and cloning from single AM fungal spores. Mycorrhizal 10 (4): 203–207.

    Article  CAS  Google Scholar 

  • Simon, L., M. Lalonde, and T.D. Bruns. 1992. Specific amplification of 18S fungal ribosomal gene from vesicular-arbuscular endomycorrhizal fungi colonizing roots. Applied and Environment Microbiology 58 (1): 291–295.

    Article  ADS  CAS  Google Scholar 

  • Sivakumar, N. 2013. Effect of edaphic factors and seasonal variation on spore density and root colonization of arbuscular mycorrhizal fungi in sugarcane fields. Annales De Microbiologie 63: 151–160.

    Article  Google Scholar 

  • Tahiri-Alaoui, A., G. Lingua, A. Avrova, S. Sampò, A. Fusconi, J. Antoniw, and G. Berta. 2002. A cullin gene is induced in tomato root s forming arbuscular mycorrhizae. Canadian Journal of Botany 80 (6): 607–616.

    Article  CAS  Google Scholar 

  • Tan, H.W. 2009. Fertilization management on sugarcane. Beijing, China: China Agriculture Press.

    Google Scholar 

  • Tawaraya, K., M. Saito, M. Morioka, and T. Wagatsuma. 1994. Effect of phosphate application to arbuscular mycorrhizal onion on the development and succinate-dehydrogenase activity of internal hyphae. Soil Science and Plant Nutrition 40 (4): 667–673.

    Article  CAS  Google Scholar 

  • Tripathi, P., A. Chandra, and J. Prakash. 2022. Changes in physio-biochemical attributes and dry matter accumulation vis a vis analysis of genes during drought and stress recovery at tillering stage of sugarcane. Acta Physiologiae Plantarum 44: 3.

    Article  CAS  Google Scholar 

  • Vandenkoornhuyse, P., R. Husband, T.J. Daniell, I.J. Watson, J.M. Duck, A.H. Fitter, and J.P.W. Young. 2002. Arbuscular mycorrhizal community composition associated with two plant species in a grassland ecosystem. Molecular Ecology 11 (8): 1555–1564.

    Article  CAS  PubMed  Google Scholar 

  • Wan, M.T., J.E. Rahe, and R.G. Watts. 1998. A new technique for determining the sublethal toxicity of pesticides to the vesicular-arbuscular mycorrhizal fungus Glomus intraradices. Environmental Toxicology and Chemistry 17 (7): 1421–1428.

    CAS  Google Scholar 

  • Wang, Y.Z., M.Q. Zhang, Y.Q. Ke, T.G. Pan, and S.K. Zheng. 1995a. Effects of the mycorrhiza preinoculation on the root development of sugarcane. J Fujian Agric and Forestry University  24 (3): 318–322.

    Google Scholar 

  • Wang, Y.Z., M.Q. Zhang, Y.Q. Ke, T.G. Pan, Z.P. Huang, and W. Liu. 1995b. Effects of the mycorrhiza preinoculation on the active oxygen metabolism of sugarcane leaves under water stress conditions. Chinese Journal of Economic-Agriculture 3 (2): 10–15.

    CAS  Google Scholar 

  • Wang, M.Y., S.T. Liu, and R.J. Liu. 2006. P-tolerance of arbuscular mycorrhizal fungi in soil under long-term fertilization. Acta Pedologia Sinica 43 (6): 1056–1059.

    Google Scholar 

  • Wang, S., M. Tang, Z.C. Niu, and H.Q. Zhang. 2008. Relationship between AM fungi resources of rare medicinal plants and soil factors in Lishan Mountain. Acta Botanica Boreali-Occidentalia Sinica 28 (2): 355–361.

    Google Scholar 

  • Wang, Q., Q. Wang, X.J. Wang, L. Zhang, and L. Jin. 2015. Research progress on ecological function of arbuscular mycorrhizal network. Chinese Journal of Applied Ecology 26 (7): 2192–2202.

    CAS  PubMed  Google Scholar 

  • Wilson, G.W.T., C.W. Rice, M.C. Rillig, A. Springer, and D.C. Hartnett. 2009. Soil aggregation and carbon sequestration are tightly correlated with the abundance of arbuscular mycorrhizal fungi: results from long-term field experiments. Ecology Lett 12: 452–461.

    Article  Google Scholar 

  • Xiang, D., T.L. Xu, H. Li, and B.D. Chen. 2017. Ecological distribution of arbuscular mycorrhizal fungi and the influencing factors. Acta Ecologica Sinica 37 (11): 3597–3606.

    Google Scholar 

  • Zhang, Z.Y., H.W. Tan, and Q.X. Zhou. 1998. Study on soil potassium status and balanced fertilization in Guangxi. Beijing, China: China Agriculture Press.

    Google Scholar 

  • Zhao, X., B.W. Wang, and X.F. Yan. 2006. Effect of arbuscular mycorrhiza on camptothecin content in Camptotheca acuminata seedlings. Acta Ecologica Sinica 26 (4): 1057–1062.

    CAS  Google Scholar 

  • Zhu, Y., Y.X. Liu, X.F. Gong, Y.L. Chen, A.T. Ren, R.J. Liu, L. Jin, and Y.C. Xiong. 2016. Symbiosis between three arbuscular mycorrhizal fungi and three host plants. Microbiology China 43 (3): 527–533.

    Google Scholar 

Download references

Funding

Guangxi Natural Science Fund key projects (2019GXNSFDA245013), Guangxi Academy of Agricultural Sciences project (2021YT097; 2023YM96, GNK2021009), Project of Guangxi Sugarcane Innovation team of National Modern Agriculture Industry Technology System (gjnytxgxcxtd-2021–03-01), and Guangxi Key R&D Program project (GK-AA22117009).

Author information

Author notes

  1. Jin-Lian Zhang, Juan Song and Yi-Hao Kang have contributed equally to this work and share first authorship.

Authors and Affiliations

  1. Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, Guangxi, China

    Jin-Lian Zhang, Juan Song, Yi-Hao Kang, Yun-Ying Wen, Ying-Jie Nong, Qian Wang, Xiao-Juan Qin & Ting-Su Chen

  2. Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning, 530007, Guangxi, China

    Yang-Rui Li

Authors
  1. Jin-Lian Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Juan Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yi-Hao Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yun-Ying Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ying-Jie Nong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qian Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xiao-Juan Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ting-Su Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yang-Rui Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xiao-Juan Qin, Ting-Su Chen or Yang-Rui Li.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, JL., Song, J., Kang, YH. et al. The Community of Arbuscular Mycorrhizal Fungi in Sugarcane Rhizosphere Soils in Guangxi, China. Sugar Tech 26, 131–142 (2024). https://doi.org/10.1007/s12355-023-01309-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12355-023-01309-5

Keywords

Search

Navigation