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A Simple Precipitation Approach for Isolation and Enrichment of Sugarcane Streak Mosaic Virus

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Abstract

Sugarcane streak mosaic virus (SCSMV) is a more prevalent sugarcane infecting virus reducing the total sugar production of the world which necessitates rapid screening and detailed studies on SCSMV pathogenicity. A simple reliable, user friendly approach for purification and enrichment of SCSMV from infected sugarcane leaf tissue was developed as alternative to ultracentrifugation approach. The quality and fold enrichment of viral particle was confirmed by RT-PCR and sequence analysis of SCSMV coat protein region. This approach offers 20.03-fold increase in sensitivity for their detection and high viral: host genome ratio of 102 and 103 in infected leaf tissue and purified viral fraction, respectively.

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Acknowledgments

Authors are thankful to Dr. R. Vasanthakumar, Chairman, Shri. K. Murigaiah, Advisor and Dr. P. Lakshmanaperumalsamy, Registrar, Karpagam Univerisity, Coimbatore, Tamil Nadu, India for their encouragement and support during the course of study.

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Authors and Affiliations

  1. Plant Functional Genomics Unit, Department of Biotechnology, Karpagam University, Eachanari Post, Coimbatore, 641021, Tamil Nadu, India

    Viswanathan Chandran & Prabu Gajjeraman

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  1. Viswanathan Chandran
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  2. Prabu Gajjeraman
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Correspondence to Prabu Gajjeraman.

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Chandran, V., Gajjeraman, P. A Simple Precipitation Approach for Isolation and Enrichment of Sugarcane Streak Mosaic Virus . Sugar Tech 15, 417–419 (2013). https://doi.org/10.1007/s12355-013-0225-x

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  • DOI: https://doi.org/10.1007/s12355-013-0225-x

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