The Transcription Factor, α1ACT, Acts Through a MicroRNA Network to Regulate Neurogenesis and Cell Death During Neonatal Cerebellar Development

MicroRNAs, a class of small RNA regulators, function throughout neurodevelopment, from neural stem cell neurogenesis to neuronal maturation, synaptic formation, and plasticity. α1ACT, a transcription factor (TF), plays a critical role in neonatal cerebellar development by regulating an ensemble of genes. Of these, ChIP-seq analysis matched near 50% genes directly regulated by α1ACT. Yet, more than half the regulated transcripts lacked direct interaction with α1ACT. To investigate whether α1ACT acts through a microRNA network, we studied α1ACT-associated simultaneous miRNA:mRNA transcriptome profiles, using miRNA-seq paired with RNA-seq. Thirty-one differentially expressed miRNAs (DEMs) associated with α1ACT-regulated differentially expressed genes (DEGs) were profiled in α1ACT-overexpressing PC12 cells and were further validated in neonatal transgenic mouse cerebellum overexpressing α1ACT in a context-dependent manner. Here, we also demonstrated that α1ACT facilitates neurogenesis and development of dendritic synapses and is partially a result of the downregulation of the miR-99 cluster, miR-143, miR-23, miR-146, miR-363, and miR-484. On the other hand, the miR-181, miR-125, and miR-708 clusters were upregulated by α1ACT, which inhibit MAPK signaling and cell death pathways by targeting Ask1, Odc1, Atf4, and Nuf2 for decreased expression. MiR-181a-5p was verified as the most abundant DEM in neonatal cerebellum, which was further induced by α1ACT. Overall, under α1ACT modulation, up-/downregulated miRNA clusters with their paired target genes may form a regulatory network controlling the balance between the neuronal proliferation, differentiation, and cell death in the cerebellum to promote neonatal development. Our findings concerning the α1ACT-related miRNA/mRNA expression profiles in neonatal cerebellum may inform future investigations for cerebellar development. Supplementary Information The online version contains supplementary material available at 10.1007/s12311-022-01431-2.


Introduction
The proliferation of neuronal precursor cells and the division of immature neurocytes are key to cerebellar morphogenesis during embryonic and fetal development [1,2]. The neonatal cerebellum still retains considerable proliferative capacity [3][4][5] and is sustained by the activation of various transcriptional regulators [5][6][7] and endogenous microRNA(miRNA) mechanisms [8][9][10][11]. The maturation of the Purkinje cell (PC) dendritic tree and soma is also extended to early postnatal life, when cerebellar neurocytes still retain the ability to differentiate and proliferate [12][13][14]. However, the molecular mechanisms that induce differentiation and maturation of neonatal neurocytes toward cerebellar cells remain obscure.
MiRNAs are a class of short noncoding RNAs controlling the expression levels of their target genes at a posttranscriptional stage. At the molecular level, miRNAs exert their function by binding, with imperfect base pairing, to target sites on their specific mRNAs. This miRNA/mRNA binding either causes the inhibition of translational initiation or leads to mRNA degradation [15,16]. Thus, to gain insight into the biological role of a single miRNA, it is essential to 1 3 identify the full repertoire of its mRNA targets. The brain, which has a wide variety of neuronal and non-neuronal cell types, expresses larger number and a more distinct set of miRNAs than any other tissues [17]. The diversity and generegulatory capacity of miRNAs are particularly valuable in the neonatal cerebellum, where persistent functional specialization of neurons requires constant neuronal adaptation to environmental cues [18]. MicroRNAs that fine-tune protein synthesis have emerged as key factors in the regulation of brain and cerebellar development [19,20].
We have shown that in cerebellar PCs, the expression of α1ACT, normally co-expressed with the α1A voltage-gated channel protein by the CACNA1A gene, is required for normal motor and cerebellar development [12,21]. α1ACT, as a transcription factor, regulates a large ensemble of developmentally controlled genes, thereby orchestrating a network of pathways driving neurogenesis, synapse formation, and cell adhesion to mediate morphological and functional maturation of PCs and their connections. In previous studies, we demonstrated the mRNA expression profiles under the regulation of α1ACT, and showed that some of the mRNA expressions were under the regulation of α1ACT due to its genomic DNA binding patterns. In this study, we additionally correlated the mRNA expression profiling of α1ACT with miRNA as putative post-transcriptional regulators.
It has been suggested that the simultaneous expression profiling of miRNAs and mRNAs could be an effective strategy for miRNA target identification [22]. We performed a co-sequencing of miRNA and mRNA transcriptomes in pc12 cells with α1ACT overexpression and confirmed our target genes in cerebellar tissues. We demonstrated that miRNA and mRNA profiling is feasible to reveal regulatory mechanisms both upstream and downstream of intercellular miRNA heterogeneity and miRNA/mRNA interaction and identified several miRNAs as potential regulators.

Ethics Statement
All animal experiments were approved and carried out in accordance with the regulations and guidelines for the care and use of experimental animals at the Institutional Animal Care and Use Committee at the University of Chicago.

α1ACT Overexpression Mouse Model
All mice were housed in groups of up to five littermates in special pathogen-free facility at the University of Chicago with a 12-h light/dark cycle. The BAC-α1ACT-3xFlag mice (C57BL/6j background) that carry a truncated human CAC-NA1A gene with 4 CAG repeats was generated by M Koob and K Kenzow at the University of Minnesota. A Purkinje cell-specific Tet-transactivator (tTA) driver construct was generated by fully replacing the coding region of the mouse Purkinje cell protein 2 (Pcp2, isoform 6) gene with the "tTA-Advanced" open reading frame (TaKaRa) in the mouse genomic BAC clone RP24-186D18 (BacPacResources). The α1ACT-3xFlag fragment driven by a Tet-responsive element (TRE) 3G promoter (TaKaRa) was inserted into the SP6 end of the BAC vector sequence in the same construct. This BAC construct was then integrated in single copy into the mouse genome in a nondisrupting site 3' of the Col1A1 gene, as described [23]. C57BL6j mice were purchased from Jackson Laboratory.
Cell Lines Rat pheochromocytoma cell line pc12 was established from ATCC and cultured in F-12 K medium (ATCC, Manassas) supplemented with 10% horse serum and 2.5% fetal bovine serum (FBS) in a 37 °C, 5% CO2 incubator on a plate coated with 0.01% poly-L-lysine. For stable cell line generation, when pc12 cells reached 60% confluence, they were transfected with pcDNA3 vectors expressing C-terminal 3xFlag tag α1ACT with 11 polyglutamine repeats and empty pcDNA3 vector using TransIT 1(Mirus, Pittsburgh, PA, USA) by following the manufacturer's instruction. Transfected cells were selected with 150 mg/ml G418(GIBCO), and then stable clones were obtained.
pc12 cells were derived from a male rat pheochromocytoma which was first cultured by Greene and Tischler in 1976 [24]. pc12 cells can be sub-cultured indefinitely and develop into neuronal phenotype in response to nerve growth factor. pc12 cells also can synthesize, store, and release neurotransmitters such as DA or noradrenaline [25] [26]. The pc12 cell line has been a classic model to study the physiology and pathology of the nervous system [27]. Studies have been shown pc12 cells are similar to the primary cultured neurons and have the features of mature dopaminergic neurons [28,29] expressing ion channels and neurotransmitter receptor [30].

RNA Isolation and Quantitative Real-Time PCR
Total cellular RNA and total mouse brain and cerebellum RNA was isolated by RNeasy mini Kit and RNeasy lipid mini Kit (QIAGEN, Valencia, CA, USA), respectively, according to the manufacturer's instructions. The flowthrough obtained from the total RNA extraction was used for purifying the miRNA-enriched fraction by using the RNeasy MinElute Cleanup Kit (QIAGEN, Valencia, CA, USA), following the manufacturer's instructions. miRNA adapter ligation, reverse-transcription, and amplification were proceeded using Taqman Advanced miRNA cDNA Synthesis Kit (Applied Biosystems, Waltham, MA) by following the manufacturer's instructions. To conduct miRNA quantitative real-time PCR, each 10 μL reaction contained 0.5 μL Taqman advanced gene expression Assay (Thermal Fisher, Waltham, MA), 0.5 μL cDNA templates, 5 μl Taqman advanced gene expression Master Mix (Applied Biosystems, Waltham, MA), and 4 μL water and was ran under the following conditions: 95 °C 20 s; 95 °C 1 s, and 60 °C 30 s for 40 cycles. As a relative quantification, fold changes were measured using the ΔΔCt method, in which miR-186 and miR-192 were used as internal controls. To obtained mathematical significance, samples were triplicated, and each experiment was repeated at least three times.
mRNA cDNA was generated using SuperScript VILO cDNA Synthesis Kit (Thermal Fisher, Waltham, MA) following the manufacture's instructions. To conduct mRNA quantitative real-time PCR, each 10 μL reaction contained 0.5 μL Taqman gene expression Assay (Thermal Fisher, Waltham, MA), 0.5 μL cDNA templates, 5 μl Taqman gene expression Master Mix (Applied Biosystems, Waltham, MA), and 4 μL water and was run under the following conditions: 50 °C 2 min; 95 °C 10 min, 95 °C 15 s, and 60 °C 60 s for 40 cycles. As a relative quantification, fold changes were measured using the ΔΔCt method, where GAPDH was used as an internal control. To obtain mathematical significance, samples were triplicated, and each experiment was repeated at least three times.

RNA and Small RNA NGS Sequencing
RNA-seq was described previously [12]. Small RNA-seq was performed using Illumina TrueSeq Small RNA protocol at the University of Chicago Genomics Facility.

Western Blot
Nuclear and cytoplasmic protein from cell and mouse cerebellum were extracted using NE-PER Nuclear and Cytoplasmic Extraction Reagents following the manufacturer's protocol (Thermo Scientific, Waltham, MA). Protein concentration was tested using Bio-Rad protein assay (Bio-Rad, Hercules, CA). Twenty μg of total protein were subjected to SDS-PAGE (8%, 10% Tris-Glycine gel, Invitrogen, Grand Island, NY, USA) and transferred to a PVDF membrane (Millipore, Billerica, MA, USA). The transblotted membrane was blocked with TBS containing 5% non-fat milk and 0.05% Tween-20 for 60 min and then incubated with the primary antibody at 4 °C overnight. The membrane was then probed with HRP-conjugated secondary antibody for 1 h at room temperature and washed with TBS with 0.05% Tween20 three times. Finally, the immunoblots were detected using chemiluminescent substrate (Thermo Scientific Pierce Protein Biology Products, Rockford, IL, USA) and visualized by autoradiography.

Immunofluorescence
Immunohistochemistry was performed as previously reported [12,21]. Briefly, paraffin-embedded sections of perfused brains were de-waxed and rehydrated and then steamed for 20 min in antigen retrieval solution (Reveal; Biocare Medical, Walnut Creek, CA, USA). Sections were blocked and exposed to primary antibody for 12 h at 4 °C. After washing, fluorescent secondary antibody in PBS-T (phosphate buffered saline and 0.05% Tween-20) was added for 1 h at room temperature. Confocal fluorescence microscopy was carried out under a Leica SP8 lighting confocal microscope (Leica, Buffalo Grove, IL, USA) at the University of Chicago Integrated Light Microscopy Core.

miRNA-seq and Differential miRNA Expression Analysis
miRNA-seq reading data was proceeded by quality control using FastQC and adapter (TGG AAT TCT CGG GTG CCA AGG) trimming using Cutadapter. The miRNA raw readings were then mapped to miRBase version 22 using two webbased tools Chimira and sRNAbench [31][32][33]. Both Chimira and sRNAbench read counts were normalized and analyzed using Bioconductor R package EdgeR with a deciding criterion |fold change|> 1.5 and FDR < 0.1 to identify differentially expressed miRNAs [34]. The DE miRNAs obtained from two mapping tools were then merged for a more inclusive outcome.

miRNA Target Mining
The predicted miRNA targets were obtained from miR-Walk 2.0 by searching the miRNA binding sites in 5′-UTR, 3′-UTR, and CDS [35]. The predicted miRNA targets were then selected using the DE mRNAs as previously described [12]. Only the mRNA reversely expressed to the miRNA was selected as a target of interest.

Gene Ontology (GO) Enrichment Analysis
All the GO term enrichment analyses of the miRNA targets were conducted using an online tool named ShinyGO v0.66 [36].

miRNA GO-Term and mRNA Networking Visualization
The network visualization was done by using Cytoscape V3.8.2 [37].

Statistical Analysis
Statistical analyses were conducted using GraphPad Prism 9 software (GraphPad Software Inc., La Jolla, CA, USA). For comparisons, unpaired t tests were used to establish statistical significance. Data were expressed as the mean ± SD and written with the identification of n under appropriate figure legends, with *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

α1ACT Induces miRNA Expression Profiles
To understand the role of miRNAs in α1ACT-regulated gene expression and cell differentiation, we used small RNA-seq to examine the simultaneous expression profiles of miR-NAs in cells overexpressing α1ACT. miRNA reads were made from the same samples used for mRNA extraction in our previous at four different time points 6 h, 24 h, 3 days, and 10 days, with growth stages ranging from starting log phase to differentiating and senescent cell, post-plating of neural-crest-derived pheochromocytoma (pc12) cell lines, stably expressing pcDNA3 vector (pc12 pcDNA3 ), and α1ACT (pc12 α1ACT ) as described previously [12,21,25,26]. Using two mapping tools, Chimira and sRNAbench, the small RNA-seq obtained an average of 3 million mapped reads and 2.7 million miRNA reads per sample, with an average miRNA mapping rate of 88.9%. Out of a total of 764 miR-NAs present in rat miRBase version 22, 596 miRNAs were detected in at least in one of 12 sequenced groups.
The perfect binding between the seed region (5′ 2-8 nucleotides 3′) of mature miRNA and 3′-UTR of their mRNA target by Watson-Crick base pairing is considered to be the major determinant in blocking the target mRNA either by translational repression or mRNA degradation [40]. Although miRNA binding sites are most common in 3′-UTRs of mRNAs, there are some reports of miRNA interaction within the 5′-UTR, mRNA coding region, and intron-exon junctions [41,42]. In our study, we found that the distribution of DEM binding events on 3′-UTR, CDS, and 5′-UTR targeting previously reported DEGs were 27.8%, 64%, and 8.2%, respectively (Fig. 1d).

Transcription Factor α1ACT Indirectly Regulates mRNA Expression Profiles
As we reported, α1ACT is a transcription factor that orchestrates a network of pathways to mediate morphological and functional maturation of PCs and their connections. The integrated RNA-seq/ChIP-seq analysis revealed that α1ACT regulates a large ensemble of developmentally controlled genes, near 50% of which are directly involved in the development of different cerebellar compartments through direct binding [12]. The integrated ChIP-Seq and RNA-Seq data revealed that 52-67% of RNA-Seq DEGs were identified in the ChIP-Seq as bona fide gene targets of α1ACT which indicates that the rest of RNA-Seq DEGs, 424 out of 1272, are indirectly regulated by α1ACT. Two thirds of these are upregulated, and one third of DEGs are downregulated. We hypothesized that a portion of the DEGs lacking α1ACT interaction by ChIP-seq might be regulated at the post-transcriptional level through miRNAs.

α1ACT Modulates the Paired Expression Profiles of miRNA and mRNA Target Relationship
The identification of putative mRNA targets may help reveal the biological role of the differentially regulated miRNAs. For systematic miRNA target prediction, we analyzed the same set of RNA-seq and small RNA-seq data to monitor the expression of both miRNAs and candidate targets [43], using two different approaches for target identification. In the first approach, we employed a comprehensive miRNA target mining database miRWalk for target prediction, which ranks predicted miRNA target genes based on their anti-correlated expression behavior relative to their respective miRNA host genes. In the second approach, we input mRNA expression profiles from our previous study based on ChIP-seq-unrelated DEGs. Putative targets of DEMs are shown in Fig. 1e and File S1-4. Literature has suggested that a single miRNA can target several mRNAs together, and a single mRNA can be targeted by different miRNAs in a concerted manner [44]. Interestingly, 16 of 18 ChIP-sequnrelated DEGs were targeted by DEMs. The relationship between one DEG targeted by two or more the DEMs was shown in Fig. 2a. L1cam, ELmod1, and tenm2 were targeted by 9, 7, and 6 downregulated DEMs, respectively (Fig. 2a). Co-targeting of individual mRNAs by different miRNAs could potentially achieve stronger and more complex patterns of repression. The relationship that one DEM targets two or more DEGs was shown in Fig. 2b. Clustering of all co-targeting pairs revealed a group of nine predominantly brain-enriched miRNAs that share many targets (Fig. 2b). Genes that are targets of multiple miRNAs are likely to be tightly regulated and may show graded response on the basis of expression of different miRNAs [45]. A large number of miRNAs and the capacity of one miRNA regulating several transcripts suggest a complex regulatory network to fine tune the gene expression and a mechanism by which they are thought to regulate various processes [44].

α1ACT-Associated DEMs Are Enriched in the Cerebellum
Seventy percent of all known miRNAs are expressed in the brain, owing in part to the diverse types of neurons and non-neuronal cells [50]. Surprisingly, only a handful of microRNAs are expressed in a brain-specific or brainenriched manner [51].

Functional Annotation Analysis of the miRNA/mRNA Network
To systemically explore the biological function associated with the DEMs/DEGs pairs modulated by α1ACT, we applied functional enrichment analysis on the miRNA target DEGs. We used ShinyGo V0.66 [36] to conduct the Gene Ontology enrichment (GO term) analysis using Enrichment FDR < 0.1 as criteria. The downregulated DEMs, miR-23b-5p, miR-99b-5p, miR143-3p, miR146b-3p, miR-362-5p, and miR-484, target to the verified DEGs, L1cam, ELmod1, Olfm2, Npy4r, Ptger3, and Cdh2 that were increased after α1ACT overexpression. The top 5 GO terms of these DEGs were related to nervous system development and neuron morphogenesis. In this case, under α1ACT overexpression, the downregulation of these DEMs eliminates the repression for the neurogenesis-related pathways to promote neuronal development. The DEGs targeted by 3 verified upregulated DEMs, miR-181a-5p, and the miR-708-3p/5p were downregulated by α1ACT overexpression. The top 5 GO terms of these DEGs are involved in cell death, cell cycle, and DNA repair. With α1ACT overexpression, DEMs were upregulated, which leads to suppression of the respective DEGs which might further inhibit the cell death and MAP pathway. The expression profiles regulated indirectly by α1ACT appeared to act through miRNAs to both enhance neurogenesis and oppose cell death (Fig. 4).

Discussion
Here, we used an integrated analysis of mRNA and miRNA expression profiles to study the effect of α1ACT on the miRNA expression profile to infer a role for miRNA networks in the indirect control of gene expression by α1ACT during cerebellar development. We sequenced mRNAs and small RNAs and verified their expression from the identical extraction of α1ACT-overexpressing mouse cerebellum. This approach provides a more accurate The immunofluorescence staining of BAC-α1ACT mouse cerebellum. α1ACT expression in cerebellar Purkinje cells. Calbindin is in green and flag is in red. Scale bar represents 20 µm. c Fifteen of 33 DEMs were verified in α1ACT overexpression mouse cerebellum. Among them, 10 DEMs were downregulated (left), and 5 DEMs are upregulated (right). Comparison of miRNA expression level between BAC-α1ACT and C57/bl6j mice cerebellum at P22 (mouse n = 7, n = 8, respectively; experiment n = 3, *p < 0.05) using miR-186 as endogenous control. d Seventeen DEGs were verified in α1ACT overexpression mouse cerebellum. Among them, 10 DEGs are upregulated (left), and 7 DEGs were downregulated (right). Comparison of mRNA expression level between BAC-α1ACT and C57/bl6j mice cerebellum at P22 (mouse n = 7, n = 8, respectively; experiment n = 3, *p < 0.05) using beta-actin as endogenous control. e miR181 enriched in neonatal C57/bl6j mouse cerebellum using miR-186 as endogenous control (right, mouse n = 8; experiment n = 3). miRNA-181 is 16 times more enriched in the neonatal cerebellum than the brain (left, mouse n = 3; experiment n = 3) assessment of transcriptome changes on the same scale. miRNA and mRNA co-sequencing at α1ACT overexpression transcriptome opens opportunities to investigate how changes in RNA expression modulated by α1ACT might contribute to neurogenesis and synaptic formation and how this coordinated activity might play a prominent role in controlling neonatal cerebellar development. The results of this study provide further insight that α1ACT, through indirect regulation, controls the fate of cerebellar neuronal homeostasis during neonatal development by balancing the processes of neurogenesis and cell death.
Over the first 9 months of life, the weight of cerebellum increases from 5.7 to 10% of the total brain weight. Cerebellar neuronal cells possess the ability to proliferate in a short time window during fetal and early postnatal life. Numerous studies demonstrate that miRNAs function at all stages of neuronal development, ranging from the initial specification of neuronal cell types to the formation, morphological dynamic modifications, and plasticity of synaptic connections between individual neurons [20,[51][52][53][54]. There are 16 downregulated DEMs identified regulated by α1ACT in our study. miR-23b-5p, miR-99b-5p, miR-143-3p, miR-146-3p, miR-362-5p, and miR-484 were verified as increased in the neonatal cerebellum of α1ACT-overexpressing mouse. All of these miRNAs paired with the previously verified upregulated DEGs involved in neurogenesis, neuronal morphological, and synaptic plasticity [12]. This provides evidence that miR-23b-5p, miR-99b-5p, miR-143-3p, miR-146-3p, miR-362-5p, and miR-484 play essential roles in controlling cerebellar neurogenesis and migration through α1ACT activation.
Two parallel, intrinsically linked processes occur during development of the nervous system: the generation of new neurons and glia and the death of cells that are no longer required and/or are produced in excess [55][56][57]. In the neonatal cerebellum, neuronal differentiation and proliferation are active as the rate of cell death decreases [58]. One way in which this connection may be achieved is through the coupling of the neurogenetic process and cell cycle, perhaps by controlling a shared set of factors [59]. In our study,15 DEMs appear to be upregulated by α1ACT: miR-181a-5p, miR-708-5p/-3p, miR-146-3p, miR-362-5p, and miR-484 were verified in the cerebellum of the α1ACToverexpressing mouse. The DEGs paired with these DEMs are involved in cell death and cell cycle arrest including genes Ask1, Odc1, Atf4, and Nuf2 that were repressed in the cerebellum during the neonatal period. In this manner, α1ACT may represent one of the shared factors, facilitating an increase in cell proliferation with the downregulated miRNAs and an upregulation of neurogenesis genes as a neuroprotective strategy to regulate cell death in cerebellum. α1ACT plays a vital role in the homeostatically balanced process of cell loss and cell gain to generate and maintain the complex architecture of cerebellum and to allow adaptation to changing circumstances after birth.
Taking into account the fact that each miRNA can regulate, on average, the expression of 100-200 target genes [60,61] and transcriptome-wide correlation of the miRNA with mRNA expression profiles with α1ACT overexpression revealed a set of target genes showing an inverse relationship with miRNA levels. In our study, 31 DEMs and 2,365 DEMs/DEGs pairs were identified modulated by α1ACT. Most of them are multi-pairing. Those DEMs and DEGs involved in pairs contribute to neurogenesis, morphologic and synaptic formation, and cell death controlling neonatal cerebellar development. α1ACT seems to utilize the miRNA/mRNA network to regulate a group of genes in neuronal genetic process and multiple cross-talk pathways in cell death and cell cycle, which may have a significant impact on the cerebellar developmental regulatory network and ultimately the physiological processes of cerebellum.
Interestingly, two of three upregulated DEMs, miR-181a-5p and miR-708-5p both targeted to Ask1 gene. Phosphorylated Ask1, as a key member of the MAPK signaling pathway, induces activation of JNK and p38 MAPK. In turn, activated JNK and p38 MAPK regulate autophagy, thereby modulating apoptosis, proliferation, and maintaining cellular integrity [46,62]. Ask1 participates in diverse biological pathological processes, such as cell death, survival, and differentiation. It has been suggested as a therapeutic target in various diseases [63]. Accumulating evidence indicates that Ask1 plays a key role in the pathogenesis of neurodegenerative diseases (NDDs), such as Alzheimer's disease, Parkinson's disease, and Huntington's disease [64]. Meanwhile, depletion of Ask1 blunts autophagy and stabilizes the survival neuron protein [48], suggesting it may be a new point of therapeutic intervention to prevent or treat NDDs [65]. miRNAs potentially are organized into related clusters. These clusters target multiple mRNA transcripts within common cellular pathways including proliferation, differentiation, and apoptosis. A total of five clusters, miR99/100/125, miR200b/429, miR143/145, miR181a/181b, and miR362/501 clusters, were identified in our study. The miR143/145, miR200b/429, and miR362/501 clusters were downregulated, stimulating neurogenesis, migration, and synaptic formation. The miR181a/181b cluster was upregulated, leading to suppression of the neuronal cell death pathway. Further, network analysis reveals that these miRNA clusters have a capacity to coordinately regulate multiple steps within a pathway, providing a dynamic and complex control of entire pathways. We also identified several miR-NAs were highly expressed in neonatal cerebellum including miR181a/181b cluster. An increasing variety of miRNAs being identified in the cerebellum suggests a complex connection between dynamics of action and regulatory potential of miRNAs in the cerebellum.
In summary, this study discovered that miRNAs mediated by α1ACT facilitate the inverse relationship between neurogenic processes and cell death during neonatal cerebellar development. Combined with our previous findings, these studies merge the whole gene expression profiles regulated both directly and indirectly by α1ACT and reveal a unique role for α1ACT in maintaining neuronal homeostasis. In broader terms, it has recently been shown that cell turnover, including neurons, does occur in the mature central nervous system. The persistence of precursor cells that possess the functional characteristics of bona fide neural stem cells within restricted brain areas [66] provides the potential neurogenetic opportunity to treat the neurodegenerative disease in adults. Since α1ACT is able to orchestrate the ensemble of genes through the direct and indirect regulation and balance of the neurogenetic and neurodegenerative process in neonates, it is feasible that α1ACT will be a unique candidate in therapeutic strategies for NDDs.