Abstract
Cutaneous T-cell lymphomas (CTCL) are a clinically and molecularly heterogeneous class of lymphomas of the skin-homing T cell, and their genetic profiles are not fully characterized. Previously, rearrangements of the Lysine Methyltransferase 2A (KMT2A) gene have been identified as driver mutations only in acute leukemias. KMT2A plays a role in epigenetic regulation, and cancers with such rearrangements are responsive to epigenetic therapy including hypomethylating agents. Here, we report two cases of CTCL with novel genetic profiles. KMT2A rearrangements were identified in two aggressive cases of mycosis fungoides with large cell transformation. A KMT2A::DSCAML1 gene rearrangement was seen in Case 1, while a KMT2A::MAPRE1 fusion was identified in Case 2. These cases demonstrate that KMT2A rearrangements can be found in primary CTCLs rather than solely acute leukemias, illustrating the importance of correlating molecular findings with clinical and histologic features in diagnosis. Additionally, this finding suggests that the subset of CTCLs driven by aberrancy of the KMT2A pathway may be responsive to therapy with hypomethylating agents or menin inhibitors, as seen in acute leukemias.
Similar content being viewed by others
References
Fei F, Natkunam Y, Zehnder JL et al (2022) Diagnostic impact of next-generation sequencing panels for lymphoproliferative neoplasms on small-volume biopsies. Am J Clin Pathol 158:345–361. https://doi.org/10.1093/ajcp/aqac045
Ma X, Liu Y, Liu Y et al (2018) Pan-cancer genome and transcriptome analyses of 1,699 paediatric leukaemias and solid tumours. Nature 555:371–376. https://doi.org/10.1038/nature25795
Fu JF, Hsu HC, Shih LY (2005) MLL is fused to EBI (MAPREI), which encodes a microtubule-associated protein, in a patient with acute lymphoblastic leukemia. Genes Chromosom Cancer 43:206–210. https://doi.org/10.1002/gcc.20174
Choi J, Goh G, Walradt T et al (2015) Genomic landscape of cutaneous T cell lymphoma. Nat Genet 47:1011–1019. https://doi.org/10.1038/ng.3356
Kießling MK, Oberholzer PA, Mondal C et al (2011) High-throughput mutation profiling of CTCL samples reveals KRAS and NRAS mutations sensitizing tumors toward inhibition of the RAS/RAF/MEK signaling cascade. Blood 117:2433–2440. https://doi.org/10.1182/blood-2010-09-305128
Yanagi T, Nishihara H, Fujii K et al (2017) Comprehensive cancer-related gene analysis reveals that active KRAS mutation is a prognostic mutation in mycosis fungoides. J Dermatol Sci 88:367–370. https://doi.org/10.1016/j.jdermsci.2017.07.013
Park J, Yang J, Wenzel AT et al (2017) Genomic analysis of 220 CTCLs identifies a novel recurrent gain-of-function alteration in RLTPR (p.Q575E). Blood 130:1430–1440. https://doi.org/10.1182/blood-2017-02-768234
Vaqué JP, Gómez-López G, Monsálvez V et al (2014) PLCG1 mutations in cutaneous T-cell lymphomas. Blood 123:2034–2043. https://doi.org/10.1182/blood-2013-05-504308
Argyropoulos KV, Pulitzer M, Maura F et al (2020) Targeted genomic analysis of cutaneous T cell lymphomas identifies a subset with aggressive clinicopathological features. Blood Cancer J 10:116. https://doi.org/10.1038/s41408-020-00380-5
Meyer C, Burmeister T, Gröger D et al (2018) The MLL recombinome of acute leukemias in 2017. Leukemia 32:273–284. https://doi.org/10.1038/leu.2017.213
Rao RC, Dou Y (2015) Hijacked in cancer: the KMT2 (MLL) family of methyltransferases. Nat Rev Cancer 15:334–346. https://doi.org/10.1038/nrc3929
Yu BD, Hess JL, Horning SE et al (1995) Altered Hox expression and segmental identity in Mll-mutant mice. Nature 378:505–508. https://doi.org/10.1038/378505a0
Meyer C, Burmeister T, Gröger D et al (2020) Mutational landscape and clinical outcome of patients with de novo acute myeloid leukemia and rearrangements involving 11q23/KMT2A. Proc Natl Acad Sci USA 117:26340–26346. https://doi.org/10.1073/pnas.2014732117
Issa GC, Aldoss I, DiPersio J et al (2023) The menin inhibitor revumenib in KMT2A-rearranged or NPM1-mutant leukaemia. Nature. https://doi.org/10.1038/s41586-023-05812-3
Gui Y, Guo G, Huang Y et al (2011) Frequent mutations of chromatin remodeling genes in transitional cell carcinoma of the bladder. Nat Genet 43:875–878. https://doi.org/10.1038/ng.907
Ji M-M, Huang Y-H, Huang J-Y et al (2018) Histone modifier gene mutations in peripheral T-cell lymphoma not otherwise specified. Haematologica 103:679–687. https://doi.org/10.3324/haematol.2017.182444
Stomper J, Rotondo JC, Greve G, Lübbert M (2021) Hypomethylating agents (HMA) for the treatment of acute myeloid leukemia and myelodysplastic syndromes: mechanisms of resistance and novel HMA-based therapies. Leukemia 35:1873–1889
Ball BJ, Arslan S, Koller P et al (2022) Clinical experience with venetoclax and hypomethylating agents (HMA) in patients with newly diagnosed and relapsed or refractory KMT2A-Rearranged acute myeloid leukemia (AML). Leuk. Lymphoma 63:3232–3236
Krivtsov AV, Armstrong SA (2007) MLL translocations, histone modifications and leukaemia stem-cell development. Nat Rev Cancer 7:823–833
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Ethical approval
Evaluation and reporting of these cases were performed under research protocols approved by the institutional review board of Stanford University.
Informed consent
The need for informed consent is waived for this type of study under the IRB-approved protocol.
Consent for publication
For this type of study and under the IRB-approved protocol, consent for publication is not required.
Conflict of interest
The authors declare no competing interests.
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Wadsworth, P.A., Lawrence, L., Suarez, C.J. et al. Two cases of mycosis fungoides with large cell transformation with KMT2A rearrangements. J Hematopathol 16, 227–234 (2023). https://doi.org/10.1007/s12308-023-00567-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12308-023-00567-2