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Chlorophyll fluorometry in evaluating photosynthetic performance: key limitations, possibilities, perspectives and alternatives

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Abstract

Non-destructive methods for the assessment of photosynthetic parameters of plants are widely applied to evaluate rapidly the photosynthetic performance, plant health, and shifts in plant productivity induced by environmental and cultivation conditions. Most of these methods are based on measurements of chlorophyll fluorescence kinetics, particularly on pulse modulation (PAM) fluorometry. In this paper, fluorescence methods are critically discussed in regard to some their possibilities and limitations inherent to vascular plants and microalgae. Attention is paid to the potential errors related to the underestimation of thylakoidal cyclic electron transport and anoxygenic photosynthesis. PAM-methods are also observed considering the color-addressed measurements. Photoacoustic methods are discussed as an alternative and supplement to fluorometry. Novel Fourier modifications of PAM-fluorometry and photoacoustics are noted as tools allowing simultaneous application of a dual or multi frequency measuring light for one sample.

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Acknowledgements

The project was supported by the Russian Science Foundation under grant No. 22-14-00338, https://rscf.ru/project/22-14-00338/, and performed in Southern Federal University (Rostov-on-Don, Russian Federation).

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Authors and Affiliations

  1. Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, Russia

    Vladimir Lysenko, Vishnu D. Rajput, Tatyana Varduny, Elizaveta Chalenko, Olga Yadronova, Pavel Dmitriev & Tatyana Zaruba

  2. Centre of Molecular and Environmental Biology, Department of Biology, Campus of Gualtar, University of Minho, Braga, Portugal

    Rupesh Kumar Singh

  3. School of IoT Engineering, Jiangnan University, Wuxi, China

    Ya Guo

  4. Russian Research Institute for the Integrated Use and Protection of Water Resources, Rostov-on-Don, Russia

    Alexey Kosolapov & Elena Usova

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  1. Vladimir Lysenko
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Lysenko, V., D. Rajput, V., Kumar Singh, R. et al. Chlorophyll fluorometry in evaluating photosynthetic performance: key limitations, possibilities, perspectives and alternatives. Physiol Mol Biol Plants 28, 2041–2056 (2022). https://doi.org/10.1007/s12298-022-01263-8

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