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Heat and chilling induced disruption of redox homeostasis and its regulation by hydrogen peroxide in germinating rice seeds (Oryza sativa L., Cultivar Ratna)

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Abstract

Extremes of temperature (both heat and chilling) during early inbibitional phase of germination caused disruption of redox-homeostasis by increasing accumulation of reactive oxygen species (superoxide and hydrogen peroxide) and significant reduction of antioxidative defense (assessed in terms of total thiol content and activities of superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) in germinating tissues of rice (Oryza sativa L., cultivar Ratna). Imbibitional heat and chilling stress also induced oxidative damage to newly assembled membrane system by aggravating membrane lipid peroxidation and protein oxidation [measured in terms of thiobarbituric acid reactive substances (TBARS), free carbonyl content (C = O groups) and membrane protein thiol level (MPTL)]. Treatment with standardized low titer hydrogen peroxide during early imbibitional phase of germination caused significant reversal in oxidative damages to the newly assembled membrane system imposed by heat and chilling stress [evident from the data of TBARS, C = O, MPTL, ROS accumulation, membrane permeability status, membrane injury index and oxidative stress index] in seedlings of experimental rice cultivar. Imbibitional H2O2 pretreatment also caused up-regulation of antioxidative defense (activities of superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase and total thiol content) in the heat and chilling stress-raised rice seedlings. When the parameters of early growth performances were assessed (in terms of relative growth index, biomass accumulation, relative germination performance, mean daily germination, T50 value), it clearly exhibited significant improvement of early growth performances of the experimental rice cultivar. The result proposes that an ‘inductive pulse’ of H2O2 is required to switch on some stress acclimatory metabolism through which plant restores redox homeostasis and prevents or repairs oxidative damages to newly assembled membrane system caused by unfavorable environmental cues during early germination to the rice cultivar Ratna. The importance of mitigating oxidative damages to membrane lipid and protein necessary for post-germinative growth under extremes of temperature is also suggested.

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Acknowledgement

The author gratefully acknowledges University Grants Commission, New Delhi, India, for research grant (F.PSU.012/08-09). The author also expresses his gratitude to Dr. Arindam Gupta, Department of Statistics, University of Burdwan, West Bengal, India, for helping in statistical analysis of data and Dr. Samir Kumar Mukherjee, Head of the Dept. of English, Hooghly Mohsin College, West Bengal, India for his valuable suggestions in improving the English of the text. Constant encouragement of my teacher Dr. M.L. Ghosh is gratefully acknowledged.

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  1. Soumen Bhattacharjee

    Present address: Department of Botany, Krishnanagar Government College, (West Bengal Education Service), Krishnanagar, District- Nadia, West Bengal, PIN-741101, India

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  1. Post Graduate Department of Botany, Hooghly Mohsin College (West Bengal Education Service), Chinsurah, 712101, West Bengal, India

    Soumen Bhattacharjee

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Correspondence to Soumen Bhattacharjee.

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Bhattacharjee, S. Heat and chilling induced disruption of redox homeostasis and its regulation by hydrogen peroxide in germinating rice seeds (Oryza sativa L., Cultivar Ratna). Physiol Mol Biol Plants 19, 199–207 (2013). https://doi.org/10.1007/s12298-012-0159-x

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