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Lack of genetic diversity of a critically endangered important medicinal plant Chlorophytum borivilianum in Central India revealed by AFLP markers

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Abstract

Chlorophytum borivilianum is a traditional medicinal plant distributed throughout the tropics and subtropics. In the present investigation, AFLP analysis was used to assess the genetic similarity among 34 accessions. Nine primer sets of AFLP amplified 612 fragments, of which 246 fragments were found to be polymorphic. The average number of polymorphic bands per AFLP primer pair was 27.33. The amplified fragments ranged from 50 base pairs to 600 base pairs. Significant correlation was observed between total number of amplified fragments and polymorphic bands (p > 0.05) per primers. Cluster analysis based on AFLP data revealed limited genetic variation within the thirty four accessions collected from various parts of Central Indian forests.

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Abbreviations

SSR:

Simple sequence repeat

AFLP:

Amplified fragment length polymorphism

RAPD:

Random amplified polymorphic DNA

ISSR:

Inter simple sequence repeats

RFLP:

Restriction fragment length polymorphism

PIC:

Polymorphic information content

DNA:

Deoxyribonucleic acid

PCR:

Polymerase chain reaction

UPGMA:

Unweighted pair group method with the arithmetic averaging algorithm

PCA:

Principal component analysis

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Acknowledgements

We are thankful for grants from Madhya Pradesh Minor Forest Produce Cooperative Ltd., Bhopal, MP, India and Department of Plant Physiology, Jawaharlal Nehru Agricultural University, Jabalpur, India for providing accessions of C. borivilianum.

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Authors and Affiliations

  1. Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur, 482004, India

    Niraj Tripathi, Priya Nair & Sharad Tiwari

  2. Vivekananda Parvartiya Krishi Anusandhan Sansthan, Indian Council of Agricultural Research, Almora, 263601, India

    Navinder Saini

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  1. Niraj Tripathi
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  2. Navinder Saini
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  3. Priya Nair
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  4. Sharad Tiwari
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Correspondence to Sharad Tiwari.

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Tripathi, N., Saini, N., Nair, P. et al. Lack of genetic diversity of a critically endangered important medicinal plant Chlorophytum borivilianum in Central India revealed by AFLP markers. Physiol Mol Biol Plants 18, 161–167 (2012). https://doi.org/10.1007/s12298-012-0108-8

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