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Synthesis of Gold Nanoparticles Coated with Aspartic Acid and Their Conjugation with FVIII Protein and FVIII Antibody

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Abstract

Carboxylate-modified gold nanoparticles (GNPs) were synthesized in a simple one-step process based on the reduction of tetrachloroauric acid by aspartic acid in water. GNPs were identified by UV–Vis spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy. Conjugation of protein molecules with functionalized nanoparticles was performed through electrostatic interaction. The GNP–protein conjugates were characterized by gel electrophoresis. The interaction between functionalized GNPs and protein molecules lead to conformational transition of protein structure after conjugation of protein with GNPs. This process was investigated by fluorescence spectroscopy and circular dichroism spectroscopy.

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Authors and Affiliations

  1. Pilot Biotechnology Department, Pasteur Institute of Iran, No. 358, 12 Farvardin Street, Jomhoori Avenue, 13169-43551, Tehran, Iran

    Maryam Farahnak Zarabi, Ali Farhangi, Samaneh Khademi Mazdeh, Davod Zare, Mohammad Reza Mehrabi & Azim Akbarzadeh

  2. Zanjan University, Zanjan, Iran

    Maryam Farahnak Zarabi & Zahra Ansarian

Authors
  1. Maryam Farahnak Zarabi
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  2. Ali Farhangi
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  3. Samaneh Khademi Mazdeh
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  4. Zahra Ansarian
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  5. Davod Zare
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  6. Mohammad Reza Mehrabi
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  7. Azim Akbarzadeh
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Correspondence to Azim Akbarzadeh.

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Zarabi, M.F., Farhangi, A., Mazdeh, S.K. et al. Synthesis of Gold Nanoparticles Coated with Aspartic Acid and Their Conjugation with FVIII Protein and FVIII Antibody. Ind J Clin Biochem 29, 154–160 (2014). https://doi.org/10.1007/s12291-013-0323-2

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  • DOI: https://doi.org/10.1007/s12291-013-0323-2

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