Skip to main content
SpringerLink
Log in

Halomonas jeotgali sp. nov., a new moderate halophilic bacterium isolated from a traditional fermented seafood

  • Note
  • Published:
The Journal of Microbiology Aims and scope Submit manuscript

Abstract

A moderate halophilic, Gram-negative, non-motile, rod-shape, and aerobe designated as strain HwaT was isolated from traditional fermented Korean seafood, which presented as a single cell or paired cells. Optimal growth occurred at 25°C in 10% (w/v) salts at pH 7.0–8.0; however, growth occurred in a temperature range of 10–32°C, a salts concentration of 5–25% (w/v) and pH 5.0–10.0. Tests for oxidase and catalase were positive. The cells produced poly-β-hydroxybutyric acid, but not exopolysaccharide. Based on the 16S rRNA gene sequence, not only was there low similarity between strain HwaT and all other species (94.1% similarity with H. subglaciescola DSM 4683T, 94.0% similarity with H. sulfidaeris Esulfide1T, 93.6% similarity with H. cerina SP4T and 93.0% similarity with H. halodurans DSM 5160T), but the phylogenetic analysis revealed that the isolate may be classified as a novel species belonging to the genus Halomonas in the class Gammaproteobacteria. The predominant fatty acids of strain HwaT were C18:1 ω7c, C16:0, C12:0 3-OH and C16:1 ω7c/C15:0 iso 2-OH. The DNA G+C content was calculated as 61.7 mol%. Based on phenotypic, genotypic, and phylogenetic characteristics, it is proposed that the strain designated as HwaT be assigned to the genus Halomonas as Halomonas jeotgali sp. nov. (=KCTC 22487T =JCM 15645T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anan’ina, L.N., E.G. Plotnikova, E. Gavrish, V.A. Demakov, and L.I. Evtushenko. 2007. Salinicola socius gen. nov., sp. nov., a moderately halophilic bacterium from a naphthalene-utilizing microbial association. Microbiolgy (English translation of Mikrobiologiia) 76, 369–376.

    Google Scholar 

  • Arahal, D.R., A.M. Castillo, W. Ludwig, K.H. Schleifer, and A. Ventosa. 2002. Proposal of Cobetia marina gen. nov., comb. nov., within the family Halomonadaceae, to include the species Halomonas marina. Syst. Appl. Microbiol. 25, 207–211.

    Article  PubMed  Google Scholar 

  • Arahal, D.R. and A. Ventosa. 2006. The family Halomonadaceae. Prokaryotes 6, 811–835.

    Article  Google Scholar 

  • Arahal, D.R., R.H. Vreeland, C.D. Litchfield, M.R. Mormile, B.J. Tindall, A. Oren, V. Bejar, E. Quesada, and A. Ventosa. 2007. Recommended minimal standards for describing new taxa of the family Halomonadaceae. Int. J. Syst. Evol. Microbiol. 57, 2436–2446.

    Article  CAS  PubMed  Google Scholar 

  • Aslam, Z., C.S. Lee, K.H. Kim, W.T. Im, L.N. Ten, and S.T. Lee. 2007. Methylobacterium jeotgali sp. nov., a non-pigmented, facultatively methylotrophic bacterium isolated from jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 57, 566–571.

    Article  CAS  PubMed  Google Scholar 

  • Aslam, Z., J.H. Lim, W.T. Im, M. Yasir, Y.R. Chung, and S.T. Lee. 2007. Salinicoccus jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 57, 633–638.

    Article  CAS  PubMed  Google Scholar 

  • Baker, G.C., J.J. Smith, and D.A. Cowan. 2003. Review and reanalysis of domain-specific 16S primers. J. Microbiol. Methods 55, 541–555.

    Article  CAS  PubMed  Google Scholar 

  • Ben Ali Gam, Z., S. Abdelkafi, L. Casalot, J.L. Tholozan, R. Oueslati, and M. Labat. 2007. Modicisalibacter tunisiensis gen. nov., sp. nov., an aerobic, moderately halophilic bacterium isolated from an oilfield-water injection sample, and emended description of the family Halomonadaceae Franzmann et al. 1989 emend Dobson and Franzmann 1996 emend. Ntougias et al. 2007. Int. J. Syst. Evol. Microbiol. 57, 2307–2313.

    Article  CAS  PubMed  Google Scholar 

  • Callies, E. and W. Mannheim. 1978. Classification of the Flavobacterium-Cytophaga complex on the basis of respiratory quinones and fumarate respiration. Int. J. Syst. Evol. Microbiol. 28, 14–19.

    CAS  Google Scholar 

  • Chun, J., J.H. Lee, Y. Jung, M. Kim, S. Kim, B.K. Kim, and Y.W. Lim. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57, 2259–2261.

    Article  CAS  PubMed  Google Scholar 

  • Dobson, S.J. and P.D. Franzmann. 1996. Unification of the genera Deleya (Baumann et al., 1983), Halomonas (Vreeland et al., 1980), and Halovibrio (Fendrich, 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons, 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae. Int. J. Syst. Bacteriol. 46, 550–558.

    Article  CAS  Google Scholar 

  • Franzmann, P.D., H.R. Burton, and T.A. Mcmeekin. 1987. Halomonas subglaciescola, a new species of halotolerant bacteria isolated from antarctica. Int. J. Syst. Evol. Microbiol. 37, 27–34.

    Google Scholar 

  • Franzmann, P.D. and B.J. Tindall. 1990. A chemotaxonomic study of members of the family Halomonadaceae. Syst. Appl. Microbiol. 13, 142–147.

    CAS  Google Scholar 

  • Gonzalez-Domenech, C.M., F. Martinez-Checa, E. Quesada, and V. Bejar. 2008. Halomonas cerina sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium. Int. J. Syst. Evol. Microbiol. 58, 803–809.

    Article  CAS  PubMed  Google Scholar 

  • Gonzalez, J.M. and C. Saiz-Jimenez. 2002. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ. Microbiol. 4, 770–773.

    Article  CAS  PubMed  Google Scholar 

  • Gram, H. 1884. Über die isolierte Färbung der Schizomyceten in Schnitt-und Trockenpräparaten. Fortschritte der Medizin 2, 185–189

    Google Scholar 

  • Hebert, A.M. and R.H. Vreeland. 1987. Phenotypic comparison of halotolerant bacteria: Halomonas halodurans sp. nov., nom. rev., comb. nov. Int. J. Syst. Evol. Microbiol. 37, 347–350.

    Google Scholar 

  • Holding, A.J. and J.G. Collee. 1971. Routine biochemical tests. Methods Microbiol. 6, 1–32.

    Article  Google Scholar 

  • Kaye, J.Z., M.C. Marquez, A. Ventosa, and J.A. Baross. 2004. Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: halophilic bacteria isolated from deep-sea hydrothermal-vent environments. Int. J. Syst. Evol. Microbiol. 54, 499–511.

    Article  CAS  PubMed  Google Scholar 

  • Kluge, A.G. and F.S. Farris. 1969. Quantitative phyletics and the evolution of anurans. Syst. Zool. 18, 1–32.

    Article  Google Scholar 

  • Koser, S.A. 1923. Utilization of the salts of organic acids by the colon aerogenes group. J. Bacteriol. 8, 493–520.

    CAS  PubMed  Google Scholar 

  • Mata, J.A., J. Martinez-Canovas, E. Quesada, and V. Bejar. 2002. A detailed phenotypic characterisation of the type strains of Halomonas species. Syst. Appl. Microbiol. 25, 360–375.

    Article  CAS  PubMed  Google Scholar 

  • MIDI. 1999. Sherlock Microbial Identification System Operating Manual, version 3.0. MIDI Inc., Newark, DE, USA.

    Google Scholar 

  • Ntougias, S., G.I. Zervakis, and C. Fasseas. 2007. Halotalea alkalilenta gen. nov., sp. nov., a novel osmotolerant and alkalitolerant bacterium from alkaline olive mill wastes, and emended description of the family Halomonadaceae Franzmann et al. 1989, emend. Dobson and Franzmann 1996. Int. J. Syst. Evol. Microbiol. 57, 1975–1983.

    Article  CAS  PubMed  Google Scholar 

  • Okamoto, T., H. Taguchi, K. Nakamura, H. Ikenaga, H. Kuraishi, and K. Yamasato. 1993. Zymobacter palmae gen. nov., sp. nov., a new ethanol-fermenting peritrichous bacterium isolated from palm sap. Arch. Microbiol. 160, 333–337.

    Article  CAS  PubMed  Google Scholar 

  • Roh, S.W., Y.D. Nam, H.W. Chang, Y. Sung, K.H. Kim, H.J. Lee, H.M. Oh, and J.W. Bae. 2007. Natronococcus jeotgali sp. nov., a halophilic archaeon isolated from shrimp jeotgal, a traditional fermented seafood from Korea. Int. J. Syst. Evol. Microbiol. 57, 2129–2131.

    Article  CAS  PubMed  Google Scholar 

  • Roh, S.W., Y.D. Nam, H.W. Chang, Y. Sung, K.H. Kim, H.M. Oh, and J.W. Bae. 2007. Halalkalicoccus jeotgali sp. nov., a halophilic archaeon from shrimp jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 57, 2296–2298.

    Article  CAS  PubMed  Google Scholar 

  • Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.

    CAS  PubMed  Google Scholar 

  • Sanchez-Porro, C., R.R. de la Haba, N. Soto-Ramirez, M.C. Marquez, R. Montalvo-Rodriguez, and A. Ventosa. 2009. Description of Kushneria aurantia gen. nov., sp. nov., a novel member of the family Halomonadaceae, and a proposal for reclassification of Halomonas marisflavi as Kushneria marisflavi comb. nov., of Halomonas indalinina as Kushneria indalinina comb. nov. and of Halomonas avicenniae as Kushneria avicenniae comb. nov. Int. J. Syst. Evol. Microbiol. 59, 397–405.

    Article  CAS  PubMed  Google Scholar 

  • Sasser, M. 1990. Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl. 20, 16.

    Google Scholar 

  • Smibert, R.M. and N.R. Krieg. 1994. Phenotypic characterization. Methods for General and Molecular Bacteriology, pp. 607–654. In N.R. Krieg, P. Gerhardt, R.G.E. Murray, and W.A. Wood (eds.). American Society for Microbiology, USA.

    Google Scholar 

  • Suh, H.K. and S.S. Yoon. 1987. A study on the regional characteristics of Korean chotkal. Kor. J. Diet Culture 2, 45–54.

    Google Scholar 

  • Tamura, K., J. Dudley, M. Nei, and S. Kumar. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596–1599.

    Article  CAS  PubMed  Google Scholar 

  • Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin, and D.G. Higgins. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876–4882.

    Article  CAS  PubMed  Google Scholar 

  • Ventosa, A., J.J. Nieto, and A. Oren. 1998. Biology of moderately halophilic aerobic bacteria. Microbiol. Mol. Biol. Rev. 62, 504–544.

    CAS  PubMed  Google Scholar 

  • Ventosa, A., E. Quesada, and F. Rodriguez-Valera. 1982. Numerical taxonomy of moderately halophilic Gram-negative rods. J. Gen. Microbiol. 128, 1959–1968.

    Google Scholar 

  • Xu, X.W., Y.H. Wu, Z. Zhou, C.S. Wang, Y.G. Zhou, H.B. Zhang, Y. Wang, and M. Wu. 2007. Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int. J. Syst. Evol. Microbiol. 57, 1619–1624.

    Article  PubMed  Google Scholar 

  • Yoon, J.H., S.H. Choi, K.C. Lee, Y.H. Kho, K.H. Kang, and Y.H. Park. 2001. Halomonas marisflavae sp. nov., a halophilic bacterium isolated from the Yellow Sea in Korea. Int. J. Syst. Evol. Microbiol. 51, 1171–1177.

    CAS  PubMed  Google Scholar 

  • Yoon, J.H., K.C. Lee, Y.H. Kho, K.H. Kang, C.J. Kim, and Y.H. Park. 2002. Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 52, 123–130.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, 130-701, Republic of Korea

    Min-Soo Kim, Seong Woon Roh & Jin-Woo Bae

Authors
  1. Min-Soo Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seong Woon Roh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jin-Woo Bae
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jin-Woo Bae.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, MS., Roh, S.W. & Bae, JW. Halomonas jeotgali sp. nov., a new moderate halophilic bacterium isolated from a traditional fermented seafood. J Microbiol. 48, 404–410 (2010). https://doi.org/10.1007/s12275-010-0032-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12275-010-0032-y

Keywords

Search

Navigation