Skip to main content
SpringerLink
Log in

Antarcticimonas flava gen. nov., sp. nov., isolated from Antarctic coastal seawater

  • Published:
The Journal of Microbiology Aims and scope Submit manuscript

Abstract

A marine bacterium, designated IMCC3175T, was isolated from a seawater sample collected off the Antarctic coast. The strain was Gram-negative, obligately aerobic, carotenoid pigment-containing, and rod-shaped bacterium that divided by binary fission. As determined by 16S rRNA gene sequence comparisons, the most closely related genera were Formosa (92.9∼93.3%), Bizionia (91.6∼93.2%), Gaetbulibacter (91.5∼92.8%), Sediminibacter (92.7%), Yeosuana (92.6%), Subsaximicrobium (92.1∼92.2%), and Gillisia (89.5∼92.2%). Phylogenese analysis based on 16S rRNA gene sequences showed that the strain formed a monophyletic clade together with the genera Sediminibacter and Subsaximicrobium but represented an independent phyletic line in this clade of the family Flavobacteriaceae. The DNA G+C content of the strain was 37.3 mol%. The major respiratory quinone was MK-6 and the predominant cellular fatty acids were C16:1 ω7c and/or iso-C15:0 2-OH (12.8%), anteiso-C15:0 (9.4%), and iso-C16:1 (9.4%). Low 16S rRNA gene sequence similarity, formation of a distinct phylogenetic branch, and several phenotypic characteristics, including a narrow range of temperature and salinity for growth, differentiated strain IMCC3175T from other related genera in the family Flavobacteriaceae. Therefore the name Antarcticimonas flava gen. nov., sp. nov. is proposed, with strain IMCC3175T (=KCCM 42713T =NBRC 103398T) as the type strain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bernardet, J.F., Y. Nakagawa, and B. Holmes. 2002. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int. J. Syst. Evol. Microbiol. 52, 1049–1070.

    Article  CAS  PubMed  Google Scholar 

  • Bowman, J.P. 2000. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int. J. Syst. Evol. Microbiol. 50, 1861–1868.

    CAS  PubMed  Google Scholar 

  • Bowman, J.P., S.A. McCammon, J.L. Brown, P.D. Nichols, and T.A. McMeekin. 1997a. Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from antarctic lacustrine and sea ice habitats. Int. J. Syst. Bacteriol. 47, 670–677.

    CAS  PubMed  Google Scholar 

  • Bowman, J.P., S.A. McCammon, M.V. Brown, D.S. Nichols, and T.A. McMeekin. 1997b. Diversity and association of psychrophilic bacteria in Antarctic sea ice. Appl. Environ. Microbiol. 63, 3068–3078.

    CAS  PubMed  Google Scholar 

  • Bowman, J.P., S.A. McCammon, T. Lewis, J.H. Skerratt, J.L. Brown, D.S. Nichols, and T.A. McMeekin. 1998. Psychroflexus torquis gen. nov., sp. nov., a psychrophilic species from Antarctic sea ice, and reclassification of Flavobacterium gondwanense (Dobson et al. 1993) as Psychroflexus gondwanense gen. nov., comb. nov. Microbiology 144, 1601–1609.

    Article  CAS  PubMed  Google Scholar 

  • Bowman, J.P. and D.S. Nichols. 2002. Aequorivita gen. nov., a member of the family Flavobacteriaceae isolated from terrestrial and marine Antarctic habitats. Int. J. Syst. Evol. Microbiol. 52, 1533–1541.

    Article  CAS  PubMed  Google Scholar 

  • Bowman, J.P. and D.S. Nichols. 2005. Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia. Int. J. Syst. Evol. Microbiol. 55, 1471–1486.

    Article  CAS  PubMed  Google Scholar 

  • Cho, J.C. and S.J. Giovannoni. 2004. Cultivation and growth characteristics of a diverse group of oligotrophic marine Gammaproteobacteria. Appl. Environ. Microbiol. 70, 432–440.

    Article  CAS  PubMed  Google Scholar 

  • Chun, J., J.H. Lee, Y. Jung, M. Kim, S. Kim, B.K. Kim, and Y.W. Lim. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57, 2259–2261.

    Article  CAS  PubMed  Google Scholar 

  • Eilers, H., J. Pernthaler, F.O. Glöckner, and R. Amann. 2000. Culturability and in situ abundance of pelagic bacteria from the North Sea. Appl. Environ. Microbiol. 66, 3044–3051.

    Article  CAS  PubMed  Google Scholar 

  • Einen, J. and L. Øvreås. 2006. Flaviramulus basaltis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seafloor basalt. Int. J. Syst. Evol. Microbiol. 56, 2455–2461.

    Article  CAS  PubMed  Google Scholar 

  • Felsenstein, J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. J. Mol. Evol. 17, 368–376.

    Article  CAS  PubMed  Google Scholar 

  • Fitch, W.M. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Zool. 20, 406–416.

    Article  Google Scholar 

  • Garrity, G.M. and J.G. Holt. 2001. The road map to the manual, p. 119–166. In D.R. Boone, R.W. Castenholz, and G.M. Garrity (eds.), Bergey’s Manual of Systematic Bacteriology, 2nd ed. Springer, New York, N.Y., USA.

    Google Scholar 

  • Glockner, F.O., B.M. Fuchs, and R. Amann. 1999. Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl. Environ. Microbiol. 65, 3721–3726.

    CAS  PubMed  Google Scholar 

  • Gosink, J.J., C.R. Woese, and J.T. Staley. 1998. Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga-Flavobacterium-Bacteroides group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov. Int. J. Syst. Bacteriol. 48, 223–235.

    Article  PubMed  Google Scholar 

  • Ivanova, E.P., Y.V. Alexeeva, S. Flavier, J.P. Wright, N.V. Zhukova, N.M. Gorshkova, V.V. Mikhailov, D.V. Nicolau, and R. Christen. 2004. Formosa algae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae. Int. J. Syst. Evol. Microbiol. 54, 705–711.

    Article  CAS  PubMed  Google Scholar 

  • Jorgensen, J.H., J.D. Turnidge, and J.A. Washington. 1999. Antibacterial susceptibility tests: dilution and disk diffusion methods, p. 1526–1543. In P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover, and R.H. Yolken (eds.), Manual of Clinical Microbiology, 7th ed. American Society for Microbiology, Washington, D.C., USA.

    Google Scholar 

  • Jukes, T.H. and C.R. Cantor. 1969. Evolution of protein molecules, p. 21–32. In H.N. Munro (ed.), Mammalian Protein Metabolism. Academic Press, New York, N.Y., USA.

    Google Scholar 

  • Jung, S.Y., S.J. Kang, M.H. Lee, S.Y. Lee, T.K. Oh, and J.H. Yoon. 2005. Gaetbulibacter saemankumensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat sediment in Korea. Int. J. Syst. Evol. Microbiol. 55, 1845–1849.

    Article  CAS  PubMed  Google Scholar 

  • Khan, S.T., Y. Nakagawa, and S. Harayama. 2007. Sediminibacter furfurosus gen. nov., sp. nov. and Gilvibacter sediminis gen. nov., sp. nov., novel members of the family Flavobacteriaceae. Int. J. Syst. Evol. Microbiol. 57, 265–269.

    Article  PubMed  Google Scholar 

  • Komagata, K. and K. Suzuki. 1987. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol. 19, 161–203.

    Article  CAS  Google Scholar 

  • Kwon, K.K., H.S. Lee, H.B. Jung, J.H. Kang, and S.J. Kim. 2006a. Yeosuana aromativorans gen. nov., sp. nov., a mesophilic marine bacterium belonging to the family Flavobacteriaceae, isolated from estuarine sediment of the South Sea, Korea. Int. J. Syst. Evol. Microbiol. 56, 727–732.

    Article  CAS  PubMed  Google Scholar 

  • Kwon, K.K., S.J. Lee, J.H. Park, T.Y. Ahn, and H.K. Lee. 2006b. Psychroserpens mesophilus sp. nov., a mesophilic marine bacterium belonging to the family Flavobacteriaceae isolated from a young biofilm. Int. J. Syst. Evol. Microbiol. 56, 1055–1058.

    Article  CAS  PubMed  Google Scholar 

  • Lau, S.C., M.M. Tsoi, X. Li, I. Plakhotnikova, S. Dobretsov, K.W. Lau, M. Wu, P.K. Wong, J.R. Pawlik, and P.Y. Qian. 2005. Winogradskyella poriferorum sp. nov., a novel member of the family Flavobacteriaceae isolated from a sponge in the Bahamas. Int. J. Syst. Evol. Microbiol. 55, 1589–1592.

    Article  CAS  PubMed  Google Scholar 

  • Lee, S.D. 2007. Tamlana crocina gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae, isolated from beach sediment in Korea. Int. J. Syst. Evol. Microbiol. 57, 764–769.

    Article  CAS  PubMed  Google Scholar 

  • Ludwig, W., O. Strunk, R. Westram, L. Richter, H. Meier, Yadhukumar, A. Buchner, T. Lai, S. Steppi, G. Jobb, W. Förster, I. Brettske, S. Gerber, A.W. Ginhart, O. Gross, S. Grumann, S. Hermann, R. Jost, A. König, T. Liss, R. Lüssmann, M. May, B. Nonhoff, B. Reichel, R. Strehlow, A. Stamatakis, N. Stuckmann, A. Vilbig, M. Lenke, T. Ludwig, A. Bode, and K.H. Schleifer. 2004. ARB: a software environment for sequence data. Nucleic Acids Res. 32, 1363–1371.

    Article  CAS  PubMed  Google Scholar 

  • McCammon, S.A. and J.P. Bowman. 2000. Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 50, 1055–1063.

    CAS  PubMed  Google Scholar 

  • Mesbah, M., U. Premachandran, and W.B. Whitman. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39, 159–167.

    CAS  Google Scholar 

  • Nedashkovskaya, O.I., S.B. Kim, S.K. Han, C. Snauwaert, M. Vancanneyt, J. Swings, K.O. Kim, A.M. Lysenko, M. Rohde, G.M. Frolova, V.V. Mikhailov, and K.S. Bae. 2005a. Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae. Int. J. Syst. Evol. Microbiol. 55, 49–55.

    Article  CAS  PubMed  Google Scholar 

  • Nedashkovskaya, O.I., S.B. Kim, A.M. Lysenko, G.M. Frolova, V.V. Mikhailov, and K.S. Bae. 2005b. Bizionia paragorgiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the soft coral Paragorgia arborea. Int. J. Syst. Evol. Microbiol. 55, 375–378.

    Article  CAS  PubMed  Google Scholar 

  • Nedashkovskaya, O.I., S.B. Kim, M. Vancanneyt, C. Snauwaert, A.M. Lysenko, M. Rohde, G.M. Frolova, N.V. Zhukova, V.V. Mikhailov, K.S. Bae, H.W. Oh, and J. Swings. 2006. Formosa agariphila sp. nov., a budding bacterium of the family Flavobacteriaceae isolated from marine environments, and emended description of the genus Formosa. Int. J. Syst. Evol. Microbiol. 56, 161–167.

    Article  CAS  PubMed  Google Scholar 

  • Nedashkovskaya, O.I., K.K. Kwon, S.H. Yang, H.S. Lee, K.H. Chung, and S.J. Kim. 2008. Lacinutrix algicola sp. nov. and Lacinutrix mariniflava sp. nov., two novel marine alga-associated bacteria and emended description of the genus Lacinutrix. Int. J. Syst. Evol. Microbiol. 58, 2694–2698.

    Article  CAS  PubMed  Google Scholar 

  • Nedashkovskaya, O.I., M. Vancanneyt, S.B. Kim, and N.V. Zhukova. 2009. Winogradskyella echinorum sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius. Int. J. Syst. Evol. Microbiol. 59, 1465–1468.

    Article  CAS  PubMed  Google Scholar 

  • Reasoner, D.J. and E.E. Geldreich. 1985. A new medium for the enumeration and subculture of bacteria from potable water. Appl. Environ. Microbiol. 49, 1–7.

    CAS  PubMed  Google Scholar 

  • Reichenbach, H. 1989. Order I. Cytophagales Leadbetter 1974, p. 2011–2013. In J.T. Staley, M.P. Bryant, N. Pfennig, and J.G. Holt (eds.), Bergey’s Manual of Systematic Bacteriology, 8th ed. Williams & Wilkins, Baltimore, USA.

    Google Scholar 

  • Romanenko, L.A., N. Tanaka, G.M. Frolova, and V.V. Mikhailov. 2009. Winogradskyella arenosi sp. nov., a member of the family Flavobacteriaceae isolated from marine sediments from the Sea of Japan. Int. J. Syst. Evol. Microbiol. 59, 1443–1446.

    Article  CAS  PubMed  Google Scholar 

  • Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.

    CAS  PubMed  Google Scholar 

  • Shindo, K., K. Kikuta, A. Suzuki, A. Katsuta, H. Kasai, M. Yasumoto-Hirose, Y. Matsuo, N. Misawa, and S. Takaichi. 2007. Rare carotenoids, (3R)-saproxanthin and (3R,2′S)-myxol, isolated from novel marine bacteria (Flavobacteriaceae) and their antioxidative activities. Appl. Microbiol. Biotechnol. 74, 1350–1357.

    Article  CAS  PubMed  Google Scholar 

  • Swofford, D. 2002. PAUP*. Phylogenetic analysis using parsimony (and other methods). Sinauer Associates, Sunderland, Massachusetts, USA.

    Google Scholar 

  • Teather, R.M. and P.J. Wood. 1982. Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol. 43, 777–780.

    CAS  PubMed  Google Scholar 

  • Van Trappen, S., I. Vandecandelaere, J. Mergaert, and J. Swings. 2004. Gillisia limnaea gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from a microbial mat in Lake Fryxell, Antarctica. Int. J. Syst. Evol. Microbiol. 54, 445–448.

    Article  PubMed  CAS  Google Scholar 

  • Yang, S.J., Y.J. Choo, and J.C. Cho. 2007. Lutimonas vermicola gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from the marine polychaete Periserrula leucophryna. Int. J. Syst. Evol. Microbiol. 57, 1679–1684.

    Article  CAS  PubMed  Google Scholar 

  • Yi, H., H.I. Yoon, and J. Chun. 2005. Sejongia antarctica gen. nov., sp. nov. and Sejongia jeonii sp. nov., isolated from the Antarctic. Int. J. Syst. Evol. Microbiol. 55, 409–416.

    Article  CAS  PubMed  Google Scholar 

  • Yoon, J.H., S.J. Kang, M.H. Lee, and T.K. Oh. 2008. Tamlana agarivorans sp. nov., isolated from seawater off Jeju Island in Korea. Int. J. Syst. Evol. Microbiol. 58, 1892–1895.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Biology and Ocean Sciences, Inha University, Incheon, 402-751, Republic of Korea

    Seung-Jo Yang, Hyun-Myung Oh & Jang-Cheon Cho

  2. Korean Minjok Leadership Academy, Gangwondo, 225-823, Republic of Korea

    Sangyun Chung

Authors
  1. Seung-Jo Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hyun-Myung Oh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sangyun Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jang-Cheon Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jang-Cheon Cho.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yang, SJ., Oh, HM., Chung, S. et al. Antarcticimonas flava gen. nov., sp. nov., isolated from Antarctic coastal seawater. J Microbiol. 47, 517–523 (2009). https://doi.org/10.1007/s12275-009-0225-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12275-009-0225-4

Keywords

Search

Navigation