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5′ untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation

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Abstract

The σS subunit of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genes in Gram-negative bacteria. The Pseudomonas putida rpoS gene is transcribed as a monocistronic rpoS mRNA with a 368 nucleotide-long 5′ untranslated region (5′ UTR). In this study, we investigate the posttranscriptional control of RpoS synthesis using rpoS-lacZ transcriptional and translational fusions consisting of the native promoter and deletions of 5′ UTR or insertion into UTR. The differing activity of constructed translational fusions strongly indicated that the 5′ UTR is involved in the translational regulation of RpoS expression in the stationary phase. The results obtained herein demonstrated that the structure of UTR performs an important function in the translational regulation of the rpoS gene.

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References

  • Bertani, I., M. Sevo, M. Kojic, and V. Venturi. 2003. Role of GacA, LasI, RhlI, Ppk, PsrA, Vfr and ClpXP in the regulation of the stationary-phase sigma factor rpoS/RpoS in Pseudomonas. Arch. Microbiol. 180, 264–271.

    Article  PubMed  CAS  Google Scholar 

  • Birnboim, H.C. 1983. A rapid alkaline extraction method for the isolation of plasmid DNA. Methods Enzymol. 100, 243–255.

    Article  PubMed  CAS  Google Scholar 

  • De Lorenzo, V., M. Herrero, U. Jakubzik, and K.N. Timmis. 1990. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative eubacteria. J. Bacteriol. 172, 6568–6572.

    PubMed  Google Scholar 

  • Figurski, D.H. and D.R. Helinski. 1979. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76, 1648–1652.

    Article  PubMed  CAS  Google Scholar 

  • Hengge-Aronis, R. 2002. Signal transduction and regulatory mechanisms involved in control of the sigma(S) (RpoS) subunit of RNA polymerase. Microbiol. Mol. Biol. Rev. 66, 373–395.

    Article  PubMed  CAS  Google Scholar 

  • Jishage, M., A. Iwata, S. Ueda, and A. Ishihama. 1996. Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli: intracellular levels of four species of sigma subunit under various growth conditions. J. Bacteriol. 178, 5447–5451.

    PubMed  CAS  Google Scholar 

  • Kojic, M., C. Aguilar, and V. Venturi. 2002. TetR family member psrA directly binds the Pseudomonas rpoS and psrA promoters. J. Bacteriol. 184, 2324–2330.

    Article  PubMed  CAS  Google Scholar 

  • Kojic, M., B. Jovcic, A. Vindigni, F. Odreman, and V. Venturi. 2005. Novel target genes of PsrA transcriptional regulator of Pseudomonas aeruginosa. FEMS Microbiol. Lett. 246, 175–181.

    Article  PubMed  CAS  Google Scholar 

  • Mathews, D.H., J. Sabina, M. Zuker, and D.H. Turner. 1999. Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure. J. Mol. Biol. 288, 911–940.

    Article  PubMed  CAS  Google Scholar 

  • Miller, J.H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, NY, USA.

    Google Scholar 

  • Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, NY, USA.

    Google Scholar 

  • Sevo, M., E. Buratti, and V. Venturi. 2004. Ribosomal protein S1 specifically binds to the 5′ untranslated region of the Pseudomonas aeruginosa stationary-phase sigma factor rpoS mRNA in the logarithmic phase of growth. J. Bacteriol. 186, 4903–4909.

    Article  PubMed  CAS  Google Scholar 

  • Spaink, H.P., R.J.H. Okker, C.A. Wijffelmann, E. Pees, and B.J.J Lugtemberg. 1987. Promoter in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI. Plant Mol. Biol. 9, 27–39.

    Article  CAS  Google Scholar 

  • Venturi, V. 2003. Control of rpoS transcription in Escherichia coli and Pseudomonas: why so different? Mol. Microbiol. 49, 1–9.

    Article  PubMed  CAS  Google Scholar 

  • Zuker, M. 2003. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res. 31, 3406–3415.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444/a, PO Box 23, 11010, Belgrade, Serbia

    Branko Jovcic, Ljubisa Topisirovic & Milan Kojic

  2. International Centre for Genetic Engineering and Biotechnology, Area Science Park, 34012, Trieste, Italy

    Iris Bertani & Vittorio Venturi

Authors
  1. Branko Jovcic
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  2. Iris Bertani
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  3. Vittorio Venturi
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  4. Ljubisa Topisirovic
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  5. Milan Kojic
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Correspondence to Milan Kojic.

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Jovcic, B., Bertani, I., Venturi, V. et al. 5′ untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation. J Microbiol. 46, 56–61 (2008). https://doi.org/10.1007/s12275-007-0127-2

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  • DOI: https://doi.org/10.1007/s12275-007-0127-2

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