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Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy

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Abstract

Thylakoid (Tk) membranes are of unique superiority in photodynamic therapy (PDT) because they not only carry abundant chlorophylls containing photosensitizer porphyrin but also can produce O2. However, the current therapeutic performance of Tk is dramatically limited because of their poor tumor targeting and inefficient O2 production. Here, we report an immunomodulatory bio-nanovesicle of Tk membranes fused with M1 macrophage-derived extracellular vesicles (M1 EV) for efficient PDT of tumors. The hybrid nanovesicle Tk@M1 was prepared by squeezing the Tk membranes of spinach with M1 EV. The systemic study confirmed that Tk@M1 can not only actively accumulate in tumors but also effectively regulate the inactive immune microenvironment of tumors. Such activated “hot” tumors significantly enhance the PDT efficacy of Tk@M1 attributed to the increased O2 from catalase catalyzed decomposition of augmented H2O2, providing a novel idea about constructing natural systems for effective tumor treatment.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (Nos. 21874011, 91859123, and 32101140), the National Science Fund for Distinguished Young Scholars (No. 22025401), the China Postdoctoral Science Foundation (No. 2020M680396). China Postdoctoral Science Foundation (Nos. 2021TQ0037 and 2021M690405). The National Natural Science Foundation of China (No. 21904012). The authors thank Biological & Medical Engineering Core Facilities (Beijing Institute of Technology) for providing advanced equipment.

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Authors and Affiliations

  1. School of Life Science, Beijing Institute of Technology, Beijing, 100081, China

    Houli Liu, Yao Lei, Helin Zhao, Yuzhu Wu, Liping Zuo & Hai-Yan Xie

  2. School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, 100081, China

    Weidong Nie & Guanghao Wu

  3. School of Stomatology, Perking University, Beijing, 100081, China

    Ruili Yang

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  1. Houli Liu
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  2. Yao Lei
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  3. Weidong Nie
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  4. Helin Zhao
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  7. Guanghao Wu
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  8. Ruili Yang
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  9. Hai-Yan Xie
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Correspondence to Ruili Yang or Hai-Yan Xie.

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Liu, H., Lei, Y., Nie, W. et al. Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy. Nano Res. 15, 4233–4242 (2022). https://doi.org/10.1007/s12274-021-4050-3

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